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Effect of Vitamin B12 deficiency on phosphatidylethanolamine methylation in rat liver

Published online by Cambridge University Press:  09 March 2007

B. Åkesson ,
C. Fehling  and
Margaretha JÄGerstad
B. Åkesson
Affiliation:
Department of Physiological Chemistry, Department of Neurology, Dalby Community Care Research Centre, University Hospital, Lund, Sweden
C. Fehling
Affiliation:
Department of Physiological Chemistry, Department of Neurology, Dalby Community Care Research Centre, University Hospital, Lund, Sweden
Margaretha JÄGerstad
Affiliation:
Department of Physiological Chemistry, Department of Neurology, Dalby Community Care Research Centre, University Hospital, Lund, Sweden
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Abstract

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1. In vitamin B12 deficiency the activity of tetrahydropteroylglutamate methyltransferase (EC 2.1.1.13) is depressed and the synthesis of methionine is reduced. Because the methyl group of methionine is largely utilized for the methylation of phosphatidylethanolamine, we investigated the effects of vitamin B12 deficiency on phosphatidylcholine synthesis.

2. The incorporation of injected [14Clformaldehyde into liver phosphatidylcholine was reduced by approximately 50% in vitamin B12-deficient rats. Also the corresponding incorporation of 5-[14C]methyl-tetrahydrofolic acid tended to decrease. The findings are consistent with a lower conversion of these precursors to methionine.

3. The effect of the deficient methyl-group supply on phosphatidylcholine synthesis was also investigated by the injection of [14C]ethanolamine. The amount (%) of lipid-14C recovered in phosphatidylcholine was significantly reduced in vitamin B12 deficiency.

4. Chemical analysis of liver phospholipids showed that the vitamin B12-deficient rats had a higher proportion of phosphatidylethanolamine and a lower proportion of phosphatidylcholine, indicating that the impaired synthesis of phosphatidylcholine by methylation leads to changes in membrane phospholipid composition.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 40 , Issue 3 , November 1978 , pp. 521 - 527
Copyright
Copyright © The Nutrition Society 1978

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