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Effect of vitamin A deficiency on the immune response to epizootic diarrhoea of infant mice (EDIM) rotavirus infection in mice

Published online by Cambridge University Press:  09 March 2007

Faruk Ahmed ,
David B. Jones  and
Alan A. Jackson
Faruk Ahmed
Affiliation:
Department of Human NutritionUniversity of Southampton, Bassett Crescent East, Southampton S09 3TU
David B. Jones
Affiliation:
Department of Pathology, University of Southampton, Bassett Crescent East, Southampton S09 3TU
Alan A. Jackson
Affiliation:
Department of Human NutritionUniversity of Southampton, Bassett Crescent East, Southampton S09 3TU
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Abstract

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The effect of vitamin A deficiency on the immune response to epizootic diarrhoea of infant mice (EDIM) rotavirus was studied in mice. The virus was given by oral dosing or by intraperitoneal injection. For oral challenge, weanling mice were fed on either a control or vitamin A-deficient diet ad lib. or pair-fed the control diet to the intake of the vitamin A-deficient group. A fourth group was fed on the vitamin Adeficient diet ad lib. for 10 weeks and then refed the control diet for 2 weeks. On day 77, mice were each given 30 μl EDIM rotavirus orally and the animals were killed and examined 1 week later. The delayedtype hypersensitivity (DTH) response to picryl chloride was measured as an index of cell-mediated immunity. For intraperitoneal challenge, weanling mice were fed on either the control diet or the vitamin A-deficient diet ad lib. or pair-fed the control diet to the intake of the vitamin A-deficient group. On day 77, mice were each injected intraperitoneally with 30 μl EDIM rotavirus and 1 week later antibody production was measured. In both experiments the body-weight, liver and serum vitamin A levels of the vitamin A-deficient group were significantly lower than the control or pair-fed groups. Following oral dosing the serum antibody levels specific to rotavirus were statistically significantly lower in vitamin Adeficient animals than the control or pair-fed groups. Vitamin A-deficient mice also showed an impaired DTH response compared with the control and pair-fed animals. Animals refed vitamin A for a short period showed a partial restoration of the antibody response. Following intraperitoneal challenge no statistically significant changes were observed in the serum antibody levels between any of the dietary groups. It is concluded that vitamin A deficiency impaired antibody production when rotavirus was given orally. Vitamin A deficiency also impaired cell-mediated immunity.

Keywords

AntibodyImmune responseMouse
Type
Effects of Altered Vitamin Status
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 475 - 485
Copyright
Copyright © The Nutrition Society 1991

References

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