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Effect of the leafy vegetable Solanum nigrum on the activities of some liver drug-metabolizing enzymes after aflatoxin B1 treatment in female rats

Published online by Cambridge University Press:  09 March 2007

Paul F. Moundipa  and
Felicite Mbiapo Domngang
Paul F. Moundipa
Affiliation:
Laboratoire de Biochimie, Faculté des Sciences, B.P. 812 Yaoundé, Cameroun
Felicite Mbiapo Domngang
Affiliation:
Laboratoire de Biochimie, Faculté des Sciences, B.P. 812 Yaoundé, Cameroun
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Abstract

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Wistar albino female rats were maintained for 10 d on diets containing various levels of the vegetable Solanum nigrum. Simultaneously, they received daily intraperitoneal injections of aflatoxin B1 (AFB1) (either 0.2 or 0.4 mg/kg body-weight) diluted in propylene glycol. At the end of the experiment, all animals were killed and their serum and hepatic microsomes were prepared for assay of enzymes. Results showed that aminopyrine N-demethylase activity increased 2.5-fold with 200 (S200) and 600 (S600) g S. nigrum/kg diets. Activity of uridine diphosphate glucuronyltransferase (UDPGT) (EC 2.4.17) also increased twofold. Similar results were obtained with glutathione S-transferase (EC 2.5.1.18) activity which increased by 60% with diet S600. After AFB1 treatment, a general increase in the activities of the above enzymes was found, except for UDPGT in the group fed on diet S600. When rats were fed on the diet without S. nigrum, AFB1 induced an increase in alkaline phosphatase (ALP) (EC 3. 1. 3. 1), aspartate aminotransferase (AST) (EC 2. 6. 1. 1) and γ-glutamyltransferase (γ-GT) (EC 2. 3. 2. 2) levels in the serum. AFB1 also induced increases in serum ALP and γ-GT levels when rats were fed on diet S600

Keywords

Aflatoxin B1Liver drug-metabolizing enzymesDetoxificationRat
Type
Diet and Liver Metabolism
Information
British Journal of Nutrition , Volume 65 , Issue 1 , January 1991 , pp. 81 - 91
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Alpert, M. E., Hutt, M. S. R., Wogan, G. N. & Davison, C. S. (1971). Association between aflatoxin content of food and hepatoma frequency in Uganda. Cancer 28, 253–260.3.0.CO;2-G>CrossRefGoogle ScholarPubMed
Appleton, D. S. & Campbell, T. C. (1983). Effect of high and low protein on the dosing and post-dosing periods of aflatoxin B1-induced preneoplastic lesion development in rat. Cancer Research 43, 2150–2154.Google Scholar
Aströn, M. A., Meijer, J. & Depierre, J. W. (1983). Characterisation of microsomal cytochrome P-450 species in rat by 2-acetylaminofluorene. Cancer Research 43, 342–348.Google Scholar
Baars, A. J., Jansen, M. & Breimer, O. D. (1978). The influence of phenobarbital, 3-methylcholanthrene and 2, 3, 7, 8-tetra-chlorodibenzo-p-dioxin on glutathione S-transferase activity of rat liver cytosol. Biochemical Pharmacology 27, 2487–2494.CrossRefGoogle Scholar
Bock, K. W., Burcell, B., Dutton, G. J., Hanninen, O., Mulder, G. J., Owens, I. S., Siest, G. & Tephly, T. R. (1983). UDP-glucuronosyltransferase activities. Guidelines for consistent interim terminology and assay conditions. Biochemical Pharmacology 32, 953–955.CrossRefGoogle ScholarPubMed
Campbell, T. C. & Hayes, J. R. (1974). The role of nutrition in the drug-metabolizing enzyme system. Pharmacological Reviews 26, 171–197.Google ScholarPubMed
Campbell, T. C. & Hayes, J. R. (1976). The role of aflatoxin metabolism in its toxic lesion. Toxicology and Applied Pharmacology 35, 199–222.CrossRefGoogle ScholarPubMed
Degen, G. H. & Neumann, H. G. (1978). The major metabolite of aflatoxin B1 in the rat is a glutathione conjugate. Chemical Biological Interactions 22, 239–255.Google Scholar
Dickerson, J. W. T., Basu, T. K. & Parke, D. V. (1976). Effect of protein-energy nutrition on the activity of hepatic microsomal drug-metabolizing enzymes in growing rats. Journal of Nutrition 106, 258–264.CrossRefGoogle ScholarPubMed
Domngang, F. (1985). Détoxification des aflatoxines par o-déméthylase chez les rats consommant les légume-feuilles, S nigrum et Amaranthus hybridus (Aflatoxin detoxification by the o-demethylase in rats fed leafy vegetables, Solanum nigrum and Amaranthus hybridus). Annales Faculté des Sciences: Biologie Biochimie 3, 81–89.Google Scholar
Domngang, F. & Bassir, O. (1981). Studies on the in vitro metabolism of aflatoxin B1 and G1 in the liver of rats fed at various levels of vitamin C. Biochemical Pharmacology 30, 3317–3322.Google Scholar
Domngang, F., Kamdem, L. & Moundipa, P. (1984). Présence des aflatoxines dans certains aliments consommés au Cameroun. (Aflatoxins in some commonly consumed foods in Cameroon). Annales Faculté des Sciences: Biologie Biochimie 2, 93–101.Google Scholar
Domngang, F., Tite, M. & Tchounwou, B. (1988). Activities of some metabolic enzymes in rats fed local vegetable (Amaranthus hybridus) and aflatoxin B1 (AFB1) diet. Nigerian Journal of Nutritional Sciences 9, 37–45.Google Scholar
Editorial (1969). Alarm about aflatoxin. Nature 212, 1512.Google Scholar
Hashmi, R. S., Siddiqui, A. M., Kachole, M. S. & Pawar, S. S. (1986). Alterations in hepatic microsomal mixed-function oxidase system during different levels of food restriction in adult male and female rats. Journal of Nutrition 116, 682–688.CrossRefGoogle ScholarPubMed
Kamdem, L. (1982). Association aflatoxin B1-oestroprogestatif. Effet sur la gamma-glutamyltransferase et sur les enzymes du métabolisme des xénobiotiques. (Interaction between aflatoxin B1 and a contraceptive agent. Effects on gamma-glutamyltransferase and some drug-metabolizing enzymes). Thèse de doctorat, Université de Nancy I.Google Scholar
Kamdem, L., Magdalou, J. & Siest, G. (1981 a). Effects of aflatoxin B1 on the activity of drug-metabolizing enzymes in rat liver. Toxicology and Applied Pharamacology 60, 570–578.CrossRefGoogle ScholarPubMed
Kamdem, L., Magdalou, J. & Siest, G. (1981 b). Effects of aflatoxin B1 on hepatic drug-metabolizing enzymes in female rats. Interaction with a contraceptive agent. Xenobiotica 11, 275–279.CrossRefGoogle ScholarPubMed
Kamdem, L., Magdalou, J. & Siest, G. (1982). Differential toxicity of aflatoxin B1 in male and female rats: relationship with hepatic drug-metabolizing enzymes. Biochemical Pharmacology 31, 3057–3062.CrossRefGoogle ScholarPubMed
Krishnamachari, K. A., Bhat, R. V., Nagaragan, V. & Tilak, T. B. (1975). Hepatitis due to aflatoxicosis. An out break in Western India. Lancet i, 1061–1062.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Madhavan, T. V. & Gopalan, C. (1965). Effects of dietary protein and aflatoxin liver injury in weanling rats. Archives of Pathology 80, 123–126.Google ScholarPubMed
Madhavan, T. V. & Gopalan, C. (1968). The effect of dietary protein on carcinogenesis of aflatoxin. Archives of Pathology 85, 133–137.Google ScholarPubMed
Nash, T. (1953). The colorimetric estimation of formaldehyde by means of the Hantzsh reaction. Biochemical Journal 55, 416–421.CrossRefGoogle Scholar
Neal, G. E., Judah, D. J., Stirpe, F. & Patterson, D. S. P. (1981). The formation of 2, 3-dihydroxy-2, 3-dihydro-aflatoxin B1 by liver microsomes isolated from certain avian and mammalian species and the possible role of this metabolite in the acute toxicity of aflatoxin B1. Toxicology and Applied Pharmacology 58, 431–437.Google Scholar
Newberne, P. M. & Butler, W. H. (1969). Acute and chronic effects of aflatoxin on the liver of domestic and laboratory animals: a review. Cancer Research 28, 236–250.Google Scholar
Newberne, P. M. & Rogers, A. E. (1973). Rat colon carcinomas associated with aflatoxin and marginal vitamin A. American Journal of the National Cancer Institute 50, 439–449.CrossRefGoogle ScholarPubMed
Nwokolo, C. & Okonkwo, P. (1978). Aflatoxin load of common food in savanna and forest regions of Nigeria. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 321–332.CrossRefGoogle ScholarPubMed
Parke, D. V. & Ioannides, C. (1981). The role of nutrition in toxicology. Annual Review of Nutrition 1, 207–234.Google Scholar
Peers, F. G., Gilman, G. A. & Linsell, C. A. (1976). Dietary aflatoxins and human liver cancer. A study in Swaziland. International Journal of Cancer 17, 167–176.Google Scholar
Sarasin, A. & Moule, Y. (1973). In vivo effect of aflatoxin B1 on protein synthesis in rat liver. FEBS Letters 29, 329–332.CrossRefGoogle ScholarPubMed
Shiau, S. Y. & Chang, G. W. (1986). Effects of certain dietary fibers on the apparent permeability of the rat intestine. Journal of Nutrition 116, 223–232.CrossRefGoogle ScholarPubMed
Sun, T. & Chu, Y. (1984). Carcinogenesis and prevention strategy of liver cancer in areas in prevalence. Journal of Cellular Physiology 3, Suppl., 39–44.Google Scholar
Watier, B. (1982). Un équilibre alimentaire en Afrique. Comment? Paris: F. Hoffman Laroche.Google Scholar
Wogan, G. N., Edwards, G. S. & Shank, R. C. (1967). Excretion and tissue distribution of radioactivity from aflatoxin B1-14C in rats. Cancer Research 27, 1729–1736.Google Scholar