Published online by Cambridge University Press: 24 July 2007
1. Groups of twelve female rats were fed from 4 weeks of age on four different experimental dietary regimens, and on a stock diet containing 18% protein given ad lib. as a control (group 1). The rats in two of the experimental groups were malnourished either by being given a low-protein (9%) diet ad lib. (group 2), or the stock diet containing 18% protein in limited amounts to maintain weight as in group 2. These animals were designated calorie-deficient (group 3). Two other groups of twelve rats were more severely malnourished from 4 to 13 weeks of age by being given a low-protein (6%) diet ad lib. (group 4) or the stock diet in limited amounts to maintain weight as in group 4 (group 5). From 13 weeks all the rats in groups 4 and 5 were rehabilitated on stock diet ad lib.
2. Well-nourished males were introduced into the cages 1 week after the vaginas opened, and again immediately after the females had suckled successive litters. This was repeated until the females were 60 weeks old and had reared up to six litters.
3. Chronic deprivation of protein or of calories delayed puberty, reduced fertility and gave rise to smaller numbers in the litters. It also interfered with lactation.
4. More severe malnutrition for 9 weeks further delayed puberty, but when these animals were rehabilitated they produced as many young as those that had been well-nourished throughout. They lactated well and there was no carry-over of the effects of the early deficiencies.