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Effect of polyunsaturated fatty acids on the expression of transcription factor adipocyte determination and differentiation-dependent factor 1 and of lipogenic and fatty acid oxidation enzymes in porcine differentiating adipocytes

Published online by Cambridge University Press:  09 March 2007

J. M. Hsu  and
S. T. Ding
J. M. Hsu
Affiliation:
Department of Animal Science, National Taiwan University, Taipei 106, Taiwan
S. T. Ding*
Affiliation:
Department of Animal Science, National Taiwan University, Taipei 106, Taiwan
*
*Corresponding author: Dr S. T. Ding, present address, 50, Lane 155, Kee-Long Rd, Sec. 3, Taipei 106, Taiwan, fax +8862/2732 4070, email [email protected]
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Abstract

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Polyunsaturated fatty acids (FA) regulate genes involved in lipid metabolism. The effects of polyunsaturated FA on the transcription factor adipocyte determination and differentiation-dependent factor (ADD) 1 and fatty acid synthase (FAS) mRNA in differentiating porcine adipocytes were measured using a stromal vascular cell culture system. Porcine stromal vascular cells were isolated from subcutaneous adi-pose tissues and plated in Dulbecco's modified Eagle's medium (DMEM)–nutrient mixture F-12 Ham (F-12) plus fetal bovine serum (100 ml/l) for 24 h. Then cells were differentiated in DMEM–F12 plus insulin, hydrocortisone and transferrin without or with polyunsaturated FA at 6·25, 25·00 or 100·00 μm. The ADD1 mRNA was decreased by 100·00 μm-arachidonic acid, 6·25 to 100·00 μm-docosahexaenoic acid or cis-9,trans-11-conjugated linoleic acid. The polyunsaturated FA reduced the transcription rate of FAS, but not of ADD1. All three polyunsaturated FA accelerated degradation of ADD1 and FAS mRNA to reduce the abundance of ADD1 and FAS mRNA. Results also showed that polyunsaturated FA inhibit the ADD1 expression, not only of mRNA concentration, but also of mature ADD1 protein concentration, suggesting an overall reduction of ADD1 function by polyunsaturated FA. Our present experiments demonstrate that polyunsaturated FA regulate the gene expression of ADD1 and enzymes involved in lipid metabolism in porcine adipocytes.

Keywords

Acyl-CoA oxidaseAdipocyte differentiationAdipocyte determination and differentiation-dependent factor 1Arachidonic acidConjugated linoleic acidDocosahexaenoic acidFatty acid synthasePolyunsaturated fatty acidsPorcine
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 3 , September 2003 , pp. 507 - 513
Copyright
Copyright © The Nutrition Society 2003

References

Baillie, RA, Takada, R, Nakamura, M & Clarke, SD (1999) Coordinate induction of peroxisomal acyl-CoA oxidase and UCP-3 by dietary fish oil: a mechanism for decreased body fat deposition. Prostaglandins Leukot Essent Fatty Acids 60, 351–356.CrossRefGoogle ScholarPubMed
Bell, AR, Savory, R, Horley, NJ, et al. (1998) Molecular basis of non-responsiveness to peroxisome proliferators: the guinea-pig PPARalpha is functional and mediates peroxisome proliferator induced hypolipidaemia. Biochem J 332, 689–693.CrossRefGoogle ScholarPubMed
Brandt, JM, Djouadi, F & Kelly, DP (1998) Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor α. J Biol Chem 273, 23786–23792.CrossRefGoogle ScholarPubMed
Brown, MS & Goldstein, JL (1997) The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89, 331–340.CrossRefGoogle ScholarPubMed
Chen, CT, Guo, IC & Chung, BC (1995) Regulation of cholesterol side-chain cleavage cytochrome P450 in mouse testis Ledig cell line I-10. DNA Cell Biol 14, 803–810.CrossRefGoogle Scholar
Chomczynski, P & Sacchi, N (1987) Single-step method of mRNA isolation by acid guanidinum thiocyanate–phenol–chloroform extraction. Ann Biochem 162, 156–159.CrossRefGoogle Scholar
Clarke, SD (2001) Polyunsaturated fatty acid regulation of gene transcription: a molecular mechanism to improve the metabolic syndrome. J Nutr 131, 1129–1132.CrossRefGoogle ScholarPubMed
Clarke, SD & Jump, DB (1994) Dietary polyunsaturated fatty acid regulation of gene transcription. Ann Rev Nutr 14, 83–98.CrossRefGoogle ScholarPubMed
Couet, C, Delarue, J, Fitz, P, Antonine, JM & Lamisse, F (1997) Effect of dietary fish oil on body fat mass and basal fat oxidation in healthy adults. Int J Obes 21, 637–643.CrossRefGoogle ScholarPubMed
Day, DA & Tuite, MF (1998) Post-transcriptional gene regulatory mechanisms in eukaryotes: an overview. J Endocrinol 157, 361–371.CrossRefGoogle ScholarPubMed
Ding, ST, Bacon, WL & Lilburn, MS (2002 a) The development of an immunoblotting assay for the quantification of liver fatty acid-binding protein during embryonic and early post-hatch development of turkeys (Meleagridis gallopavo). Poult Sci 81, 1057–1064.CrossRefGoogle Scholar
Ding, ST, McNeel, RL & Mersmann, HJ (1999) Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo. Comp Biochem Physiol 123B, 307–318.CrossRefGoogle Scholar
Ding, ST, McNeel, RL & Mersmann, HJ (2002 b) Modulation of adipocyte determination and differentiation-dependent factor 1 by selected polyunsaturated fatty acids. In Vitro Cell Dev Biol Anim 38, 352–357.2.0.CO;2>CrossRefGoogle ScholarPubMed
Ding, ST & Mersmann, HJ (2001) Fatty acids modulate porcine adipocyte differentiation and transcripts for transcription factors and adipocyte-characteristic proteins. J Nutr Biochem 12, 101–108.Google ScholarPubMed
Ding, ST, Schinckel, AP, Weber, TE & Mersmann, HJ (2000) Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations. J Anim Sci 78, 2127–2134.CrossRefGoogle ScholarPubMed
Gondret, F, Ferre, P & Dugail, I (2001) ADD-1/SREBP-1 is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. J Lipid Res 42, 106–133.CrossRefGoogle Scholar
Hill, JO, Peters, JC, Lin, D, Yakubu, F, Greene, H & Swift, L (1993) Lipid accumulation and body fat distribution is influenced by type of dietary fat fed to rats. Int J Obes Relat Metab Disord 17, 223–236.Google ScholarPubMed
Horton, JD, Shimano, H, Hamilton, RL, Brown, MS & Goldstein, JL (1999) Disruption of LDL receptor gene in transgenic SREBP-la mice unmasks hyperlipidemia resulting from production of lipid-rich VLDL. J Clin Invest 103, 1067–1076.CrossRefGoogle Scholar
Jump, DB (2002) The biochemistry of n−3 polyunsaturated fatty acids. J Biol Chem 277, 8755–8758.CrossRefGoogle Scholar
Jump, DB & Clarke, SD (1999) Regulation of gene expression by dietary fat. Ann Rev Nutr 19, 63–90.CrossRefGoogle ScholarPubMed
Osumi, T, Ishii, N, Miyazawa, S & Hashimoto, T (1987) Isolation and structural characterization of the rat acyl-CoA oxidase gene. J Biol Chem 262, 8138–8143.CrossRefGoogle ScholarPubMed
Otten, W, Wirth, C, Iaizzo, PA & Eichinger, HM (1993) A high omega 3 fatty acid diet alters fatty acid composition of heart, liver, kidney, adipose tissue and skeleton muscle in swine. Ann Nutr Metab 37, 134–141.CrossRefGoogle Scholar
Pai, JT, Guryev, O, Brown, MS & Goldstein, JL (1998) Differential stimulation of cholesterol and unsaturated fatty acid biosyn-thesis in cells expressing individual nuclear sterol regulatory element-binding proteins. J Biol Chem 273, 26138–26148.CrossRefGoogle Scholar
Pineda, Torra I, Gervois, P & Staels, B (1999) Peroxisome proliferator-activated receptor alpha in metabolic disease, inflammation, atherosclerosis and aging. Curr Opin Lipidol 10, 151–159.Google Scholar
Price, PT, Nelson, CM & Clarke, SD (2000) Omega-3 polyunsaturated fatty acid regulation of gene expression. Curr Opin Lipidol 11, 3–7.CrossRefGoogle ScholarPubMed
Reddy, JK & Hashimoto, T (2001) Peroxisomal beta-oxidation and peroxisome proliferator-activated receptor alpha: an adaptive metabolic system. Ann Rev Nutr 21, 193–230.CrossRefGoogle ScholarPubMed
Shimomura, I, Bashmakov, Y & Horton, JD (1999) Increased levels of nuclear SREBP-1c associated with fatty livers in two mouse models of diabetes mellitus. J Biol Chem 274, 30028–30032.CrossRefGoogle ScholarPubMed
Tontonoz, P, Kim, JB, Graves, RA & Spiegelman, BM (1993) ADD1: a novel helix–loop–helix transcription factor associated with adipocyte determination and differentiation. Mol Cell Biol 13, 4753–4759.Google ScholarPubMed
Wang, X, Sato, R, Brown, MS, Hua, X & Goldstein, JL (1994) SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell 77, 53–62.CrossRefGoogle ScholarPubMed
Xu, J, Nakamura, MT, Cho, HP & Clarke, SD (1999) Sterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids. J Biol Chem 274, 23577–23583.CrossRefGoogle ScholarPubMed
Xu, J, Teran-Garcia, M, Park, JHY, Nakamura, MT & Clarke, SD (2001) Polyunsaturated fatty acids suppress hepatic sterol regulatory element binding protein-1 expression by acceleration transcript decay. J Biol Chem 276, 9800–9807.CrossRefGoogle ScholarPubMed
Yahagi, N, Shimaon, H, Hasty, AJ, et al. (1999) A crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids. J Biol Chem 274, 35840–35844.CrossRefGoogle ScholarPubMed