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Effect of polyethylene glycol on in vitro degradability ofnitrogen and microbial protein synthesis fromtannin-rich browse and herbaceous legumes

Published online by Cambridge University Press:  09 March 2007

G. Getachew ,
H. P. S. Makkar  and
K. Becker
G. Getachew
Affiliation:
Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim (480), D-70593 Stuttgart, Germany
H. P. S. Makkar
Affiliation:
Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim (480), D-70593 Stuttgart, Germany
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Abstract

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Determination of microbial degradability of N is important in formulating a sound supplementation strategy for efficient utilisation of basal as well as supplementary diet components. In vitro degradability of N (IVDN) from tannin-containing browses (Acacia cyanophylla, Acacia albida, Acioa barteri and Quercus ilex) and two herbaceous legumes (Desmodium intortum andDesmodium uncinatum) was determined using the in vitro gas-production method coupled with NH3-N measurement in the presence and absence of a tannin-binding agent (polyethylene glycol (PEG), molecular mass 6000). Addition of PEG to tannin-containing feeds significantly (P < 0·05) increased in vitro gas and short-chain fatty acid (SCFA) production, and IVDN. The use of PEG as a tannin-binding agent increased IVDN from 28 to 59, 32 to 72, 19 to 40, 32 to 73, 40 to 80, and 26 to 77 % in A. cyanophylla, A. albida, A. barteri, D. intortum,D. uncinatum and Q. ilexrespectively. There was significant correlation between total phenolic compounds (total phenol, TP; total tannin, TT) in leguminous forages and percentage increase in IVDN on addition of PEG (P < 0·05; R2 0·70 and 0·82 for TP and TT respectively). The difference in IVDN observed in the absence and presence of PEG indicates the amount of protein protected from degradation in the rumen by tannins. When measured after 24 h incubation, tannin-containing feeds incubated in absence of PEG resulted in higher microbial protein synthesis than in the presence of PEG. Addition of PEG significantly (P < 0·05) reduced the efficiency of microbial protein synthesis expressed as μmol purine/mmol SCFA.

Keywords

Polyethylene glycolProtein degradabilityTanninsMicrobial protein synthesis
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 1 , July 2000 , pp. 73 - 83
Copyright
Copyright © The Nutrition Society 2000

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