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Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats*

Published online by Cambridge University Press:  09 March 2007

Katharina E. Scholz-Ahrens ,
Yahya Açil  and
Jürgen Schrezenmeir
Katharina E. Scholz-Ahrens*
Affiliation:
Institute of Physiology and Biochemistry of Nutrition, Federal Dairy Research Centre, Hermann Weigmann Str. 1, D-24103 Kiel, Germany
Yahya Açil
Affiliation:
Department of Oral and Maxillofacial Surgery, Kiel University Hospital, Germany
Jürgen Schrezenmeir
Affiliation:
Institute of Physiology and Biochemistry of Nutrition, Federal Dairy Research Centre, Hermann Weigmann Str. 1, D-24103 Kiel, Germany
*
†Corresponding author: Dr K. E. Scholz-Ahrens, fax +49 431 609 2472, email [email protected]
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Abstract

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We investigated the effects of dietary oligofructose and Ca on bone structure in ovariectomized rats, using microradiography and histomorphometry. Ninety-six animals were allocated to seven experimental groups: G1, sham-operated; G2–G7, ovariectomized. Semi-purified diets containing 5 g Ca/kg (recommended content) without oligofructose (G1, G2) or with 25, 50 or 100 g oligofructose/kg (G3, G4, G5) or 10 g Ca/kg (high content) without oligofructose (G6) or with 50 g oligofructose/kg (G7) were fed for 16 weeks. At the recommended level of Ca, high oligofructose (G5) increased femur mineral levels in ovariectomized rats, while medium oligofructose did so at high Ca. Increasing Ca in the absence of oligofructose did not increase femur mineral content. Trabecular bone area (%) analysed in the tibia was 10·3 (SEM 1·2) (G1), 7·7 (SEM 0·6) (G2), 9·3 (SEM 0·7) (G3), 9·4 (SEM 1·0) (G4), 9·5 (SEM 0·7) (G5), 10·2 (SEM 0·8) (G6), and 12·6 (SEM 0·8) (G7). At the recommended level of Ca, 25 g oligofructose/kg prevented loss of trabecular area due to increased trabecular thickness, while 50 or 100 g oligofructose/kg increased trabecular perimeter. At high Ca, oligofructose prevented loss of bone area due to increased trabecular number but similar thickness (G7 v. G6). When Ca was raised in the presence of oligofructose (G7), trabecular area and cortical thickness were highest, while loss of trabecular connectivity was lowest of all groups. At the same time, lumbar vertebra Ca was higher; 44·0 (SEM 0·8) (G7) compared with 41·6 (SEM 0·8) (G2), 41·4 (SEM 0·7) (G4), and 40·5 (SEM 1·0) mg (G6). We conclude that ovariectomy-induced loss of bone structure in the tibia was prevented but with different trabecular architecture, depending on whether dietary Ca was increased, oligofructose was incorporated, or both. Oligofructose was most effective when dietary Ca was high.

Keywords

OligofructoseCalcium and phosphorus balanceOsteoporosisTrabecular structureBone quality
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 4 , October 2002 , pp. 365 - 377
Copyright
Copyright © The Nutrition Society 2002

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