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The effect of oestrogen and dietary phyto-oestrogens on transepithelial calcium transport in human intestinal-like Caco-2 cells

Published online by Cambridge University Press:  09 March 2007

Alice A. Cotter ,
Christopher Jewell  and
Kevin D. Cashman
Alice A. Cotter
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Ireland
Christopher Jewell
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Ireland
Kevin D. Cashman*
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Ireland Department of Medicine, University College, Cork, Ireland
*
*Corresponding author: Professor Kevin D. Cashman, fax +353 21 4270244, email [email protected]
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Abstract

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Recently, dietary phyto-oestrogens (PO) have been suggested as possible alternatives to oestrogen therapy (hormone replacement therapy) as a means of preventing bone loss associated with ovarian hormone deficiency. While PO, which exhibit oestrogen-like activity, act directly on bone cells, their protective effect on bone may be partly due to their ability to enhance Ca absorption. Therefore, the aim of the present study was to investigate the effect of 17β-oestradiol and two commonly consumed soyabean PO (genistein and daidzein) on Ca absorption in the human Caco-2 intestinal-like cell model. Caco-2 cells were seeded onto permeable filter supports and allowed to differentiate into monolayers. On day 21, the Caco-2 monolayers (n 8–18 per treatment), grown in oestrogen-replete or -deplete media, were then exposed to 10 nM-17β-oestradiol, 1 nM-1,25-dihydroxycholecalciferol, or 50 μM-genistein or -daidzein for 24 h. After exposure, transepithelial and transcellular transport of 45Ca and fluorescein transport (a marker of paracellular diffusion) were measured. As expected, 1,25-dihydroxycholecalciferol stimulated Ca absorption in Caco-2 cells, by up-regulating transcellular transport, whereas 17β-oestradiol had no effect on Ca absorption. Unexpectedly, both PO decreased Ca absorption (by about 17–19 % compared with control, P<0·05), by reducing transcellular Ca transport in Caco-2 cells grown in oestrogen-replete media. This inhibitory effect disappeared when monolayers were grown in oestrogen-deplete media. In conclusion, PO at high luminal concentrations either had no effect or reduced Ca absorption in Caco-2 cells, dependent on oestrogen status. The effect of lower concentrations of these compounds needs to be investigated.

Keywords

OestrogenPhyto-oestrogensCalcium absorptionCaco-2 cells
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 6 , June 2003 , pp. 755 - 765
Copyright
Copyright © The Nutrition Society 2003

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