Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-25T02:32:07.705Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of methods of sterilization on the nutritive value of protein in a commercial rat diet

Published online by Cambridge University Press:  25 March 2008

D. J. Ford
D. J. Ford
Affiliation:
Medical Research Council Laboratory Animals Centre, Woodmansterne Road, Carshalton, Surrey SM5 4EF
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The effect on protein quality of treating a commercial rat diet by autoclaving at various temperatures for different periods of time, or by irradiation with 2.5 or 10 Mrd, was studied. True digestibility (TD) and biological value (BV) were measured and the available and total amino acids in the diets were estimated using microbiological and chemical methods..

2. Autoclaving at 121° for 60 min reduced BV, TD and net protein utilization (NPU) more than autoclaving at 134° for 3 min. Availability of amino acids was reduced by both treatments but to a greater extent by autoclaving at 121° for 60 min. Total amino acids were essentially unaffected. Irradiation had no effect on BV, TD, NPU or total amino acids, and the availability of amino acids was also unaffected, with the exception of lysine which was slightly reduced..

3. When the diet was autoclaved at 115 or 121° for 15, 30 or 60 min, or at 134° for 3 min the availability of the amino acids was reduced with increasing time and temperature of treatment. Treatment at 134° for 3 min had an effect on available amino acids similar to treatment at 121° for 15 or 30 min..

4. Ethylene oxide fumigation of the diet caused reduced availability of histidine, methionine and tryptophan but had negligible effect on arginine, leucine and lysine..

5. It is concluded that from a practical point of view irradiation causes least damage to proteins in rodent diets. If such diets are to be autoclaved they should be supplemented with complete protein to counteract amino acid destruction.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 35 , Issue 2 , March 1976 , pp. 267 - 276
Copyright
Copyright © The Nutrition Society 1976

References

Bender, A. E. (1972). PAG Bulletin 13, 1.Google Scholar
Booth, V. H. (1971). J. Sci. Fd Agric. 22, 658.CrossRefGoogle Scholar
Bradley, J. V. (1968). Distribution – Free Statistical Tests. New Jersey: Prentice-Hall Inc.Google Scholar
Carpenter, K. J. (1960). Biochem. J. 77, 604.CrossRefGoogle Scholar
Eggum, B. O. (1969). Z. Tierphysiol. Tierevnähr. Futtermittelk. 25, 204.CrossRefGoogle Scholar
Ford, J. E. (1960). Br. J. Nutr. 14, 485.CrossRefGoogle Scholar
Ford, J. E. (1962). Br. J. Nutr. 16, 409.CrossRefGoogle Scholar
Ford, J. E. & Salter, D. N. (1966). Br. J. Nutr. 20, 843.CrossRefGoogle Scholar
Fraenkel-Conrat, H. L. (1944). J. biol. Chem. 154, 227.CrossRefGoogle Scholar
Mitchell, H. H. (1924) J. biol. Chem. 58, 873.CrossRefGoogle Scholar
Porter, G. & Lane-Petter, W. (1965). Br. J. Nutr. 19, 295.CrossRefGoogle Scholar
Schoen, A. & Hiller, H. H. (1971). Z. Tierphysiol. Tierernähr. Futtermittelk. 27, 338.CrossRefGoogle Scholar
Snedecor, G. W. (1957). Statistical Methods. Ames, Iowa: Iowa State College Press.Google Scholar
Udes, H., Hiller, H. H. & Juhr, N. C. (1971). Z. Versuchstierk. 13, 160.Google Scholar
US Pharmacopeia, XIV (1950). p. 789.Google Scholar
Windrnueller, H. G., Ackerrnan, C. J. & Engel, R. W. (1956). J. Nutr. 60, 527.CrossRefGoogle Scholar
Windmueller, H. G., Ackerman, C. J. & Engel, R. W. (1959). J. biol. Chem. 234, 895.CrossRefGoogle Scholar