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Effect of magnesium deficiency on triacylglycerol-rich lipoprotein and tissue susceptibility to peroxidation in relation to vitamin E content

Published online by Cambridge University Press:  09 March 2007

Elyett Gueux ,
Veronique Azais-Braesco ,
Laurence Bussiére ,
Pascal Grolier ,
Andrezej Mazur  and
Yves Rayssiguier
Elyett Gueux
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
Veronique Azais-Braesco
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
Laurence Bussiére
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
Pascal Grolier
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
Andrezej Mazur
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
Yves Rayssiguier
Affiliation:
Centre de Recherche en Nutrition Humaine, Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St-Genés-Champanelle, France
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Abstract

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Given the current interest in the cardiovascular complications of Mg deficiency, the aim of the present experiment was to investigate the effect of Mg deficiency on the time-course of lipoprotein oxidation and to assess whether short-term Mg deficiency results in vitamin E depletion that predisposes lipoproteins and tissues to subsequent oxidation. Weanling rats were pair-fed for 8 d with control and Mg-deficient diets respectively. Plasma triacylglycerol and α-toeopherol levels were significantly greater in Mg-deficient rats compared with control animals. The increase in plasma apolipoprotein B concentration indicated that a corresponding increase in plasma triacylglycerol-rich lipoproteins (TGRLP) occurred in Mg-deficient animals. Hyperlipaemia was associated with modifications in the composition of TGRLP. The proportion of triacylglycerols was elevated whereas that of cholesterol and protein was reduced, and Mg deficiency resulted in a slight significant reduction in α-tocopherol content. When the TGRLP fractions were subjected to in vitro Cu-induced oxidation the lipoprotein fractions from Mg-deficient rats were more susceptible to oxidative damage than lipoprotein fractions from control rats. Mg deficiency did not modify the α-tocopherol content of liver, heart and skeletal muscle. However, after exposure of tissue homogenates to Fe-induced lipid peroxidation, thiobarbituric acid-reactive substances were significantly higher in tissues from Mg-deficient rats compared with those from control rats. These results complement previous findings, showing that Mg deficiency increases the susceptibility of TGRLP and tissues to peroxidation and suggest that oxidative damage is not the result of a decrease in vitamin E antioxidant status.

Keywords

MagnesiumVitamin EPeroxidation
Type
Magnesium, vitamin E and peroxidation
Information
British Journal of Nutrition , Volume 74 , Issue 6 , December 1995 , pp. 849 - 856
Copyright
Copyright © The Nutrition Society 1995

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