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Effect of long-term high-fat feeding on energy balance and liver oxidative activity in rats

Published online by Cambridge University Press:  09 March 2007

Susanna Iossa ,
Lillà Lionetti ,
Maria P. Mollica ,
Raffaella Crescenzo ,
Antonio Barletta  and
Giovanna Liverini
Susanna Iossa
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Lillà Lionetti
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Maria P. Mollica
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Raffaella Crescenzo
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Antonio Barletta
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
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Abstract

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The purpose of this work was to study the effect of early long-term high-fat feeding on energy balance and liver oxidative activity. To this end, rats aged about 30 d were fed a high-fat or a low-fat diet for 15, 30 or 60 d. Full energy balance and energy partitioning measurements were carried out. In addition, we measured hepatic mitochondrial and peroxisomal oxidative capacities. Serum levels of free triiodothyronine (T3) and leptin were also determined. Rats fed a high-fat diet showed an increase in metabolizable energy intake as well as in energy expenditure, while lipid gain over the whole period was lower than that expected due to a decrease in metabolic efficiency. An increase in serum free T3 levels was also found in rats fed a high-fat diet after 15 and 30 d. Statistically significant correlations between serum leptin levels and body fat mass were found after 15, 30 and 60 d of high-fat feeding. Finally, no variation in hepatic mitochondrial and peroxisomal fatty acid oxidation capacity was found in rats fed a high-fat diet for 15, 30 or 60 d. In conclusion, the results of the present study show that young Wistar rats fed a high-fat diet for up to 60 d are able to counteract, at least in part, obesity development.

Keywords

High-fat dietEnergy balanceHepatic respiration
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 3 , September 2000 , pp. 377 - 385
Copyright
Copyright © The Nutrition Society 2000

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