Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-06T11:43:47.579Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of Intralipid® infusion on the human leucocyte sodium-pump in vivo

Published online by Cambridge University Press:  09 March 2007

L. L. Ng  and
T. D. R. Hockaday
L. L. Ng
Affiliation:
Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The effect of unsaturated long-chain non-esterified fatty acids (NEFA) on the human leucocyte sodium-pump was studied in vivo.

2. Plasma NEFA level was raised acutely from 0·28 (sd 0·10) to 2·54 (sd 0·48) mmol/l by infusion of ‘Intralipid 20%’ (trademark) at 90 ml/h with heparin, and the human leucocyte 22Na efflux rate constants were studied in eight normal weight males.

3. After 3 h, there was a significant lowering of the total (from 3·97 (sd 0·92) to 3·10 (sd 0·71)/h; P < 0·01) and ouabain-sensitive 22Na efflux rate constants (from 2·89 (sd 0·55) to 2·37 (sd 0·62)/h; P < 0·02). Ouabain-insensitive efflux rate constants showed a tendency to fall (from 1·08 (sd 0·51) to 0·73 (sd 0·23)/h). Leucocyte potassium content remained unchanged, but sodium content rose from 31 (sd 12) to 38 (sd 18) mmol/kg dry weight (P < 0·05). Total, ouabain-insensitive and ouabain-sensitive efflux rates did not change significantly during the Intralipid-heparin infusion.

4. Plasma insulin levels rose gradually throughout the 3 h infusion period.

5. In conclusion, NEFA, when raised to pathological levels, can inhibit the leucocyte Na-pump in vivo even in the presence of physiological levels of serum albumin, and may increase insulin secretion.

Type
Clinical and Human Nutrition Papers: Studies in Man
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 49 - 55
Copyright
Copyright © The Nutrition Society 1988

References

Ahmed, K. & Thomas, B. S. (1971). Journal of Biological Chemistry 246, 103–109.CrossRefGoogle Scholar
Ashbrook, J. D., Spector, A. A., Santos, E. C. & Fletcher, J. E. (1975). Journal of Biological Chemistry 250, 2333–2338.CrossRefGoogle Scholar
Balasse, E. O., Couturier, E. & Franckson, J. (1967). Diabetologia 3, 488–493.CrossRefGoogle Scholar
Balasse, E. O. & Ooms, H. A. (1968). Diabetologia 4, 133–135.CrossRefGoogle Scholar
Balasse, E. O. & Ooms, H. A. (1973). Diabetologia 9, 145–151.CrossRefGoogle Scholar
Bergmeyer, H. U. (1974). In Methods in Enzymatic Analysis, pp. 1446–1455, 1464–1472, 1825–1835, 1836–1839, 1840–1843 [Bergmeyer, H. U., editor]. New York and London: Academic Press.Google Scholar
Bidard, J., Rossi, B., Renaud, J. & Lazdunski, M. (1984). Biochmica et Biophysica Acta 769, 245–252.CrossRefGoogle Scholar
Blaustein, M. P. (1977). American Journal of Physiology 232, C165–C173.CrossRefGoogle Scholar
Edmondson, R. P. S., Thomas, R. D., Hilton, P. J., Patrick, J. & Jones, N. F. (1975). Lancet i, 1003–1005.CrossRefGoogle Scholar
Heagerty, A. M., Ollerenshaw, J. D., Robertson, D. I., Bing, R. F. & Swales, J. D. (1986). British Medical Journal 293, 295–297.CrossRefGoogle Scholar
Hicks, B. H., Taylor, C. I., Vij, S. K., Pek, S., Knopf, R. F., Floyd, J. C. Jr & Fajans, S. S. (1977). Metabolism 26, 1011–1023.CrossRefGoogle Scholar
Kelly, R. A., O'Hara, D. S., Mitch, W. E. & Smith, T. W. (1986). Journal of Biological Chemistry 261, 11704–11711.CrossRefGoogle Scholar
Lamers, J. M. J., Stinis, H. T., Montfoort, A. D. & Hulsmann, W. C. (1984). Biochimica et Biophysica Acta 774, 127–137.CrossRefGoogle Scholar
Lowe, D. A., Richardson, B. P., Taylor, P. & Donatsch, P. (1976). Nature 260, 337–338.CrossRefGoogle Scholar
Milner, R. D. G. & Hales, C. N. (1967) Biochimica et Biophysica Acta 135, 375–377.CrossRefGoogle Scholar
Morgan, C. R. & Lazarow, A. (1962). Proceedings of the Society of Experimental Biology and Medicine 110, 29–32.CrossRefGoogle Scholar
Murray, G. E., Nair, R. & Patrick, J. (1986). British Journal of Nutrition 56, 587–593.CrossRefGoogle Scholar
Ng, L. L. & Hockaday, T. D. R. (1986 a). Clinical Science 71, 737–742.CrossRefGoogle Scholar
Ng, L. L. & Hockaday, T. D. R. (1986 b). Clinical Endocrinology 25, 383–392.CrossRefGoogle Scholar
Puska, P., Nissinen, A., Vartianen, E., Dougherty, R., Mutanen, M., Iacono, J. M., Korhonen, H. J., Pietinen, P., Leino, U. & Moisio, S. (1983). Lancet i, 1–5.CrossRefGoogle Scholar
Schalch, D. & Kipnis, D. (1965). Journal of Clinical Investigation 44, 2010–2020.CrossRefGoogle Scholar
Shimizu, S., Inoue, K., Tani, Y. & Yamada, H. (1979). Analytical Biochemistry 98, 341–345.CrossRefGoogle Scholar
Stromblad, G. & Bjorntorp, P. (1986). Metabolism 35 323–327.CrossRefGoogle Scholar
Tamura, M., Kuwano, H., Kinoshita, T. & Inagami, T. (1985). Journal of Biological Chemistry 260, 9672–9677.CrossRefGoogle Scholar