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Effect of insulin on the utilization of propionate in gluconeogenesis in sheep

Published online by Cambridge University Press:  09 March 2007

Ronald P. Brockman
Ronald P. Brockman
Affiliation:
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0WO, Canada
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Abstract

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The effects of insulin on the utilization of propionate in glucose synthesis were studied in fed and fasted sheep. Insulin was infused at 0.04 μ/h into the mesenteric vein. Glucose was infused to prevent hypoglycaemia. The rate of incorporation of [2-14C]propionate into glucose was determined before and during insulin infusion. After 150 min of insulin infusion endogenous glucose synthesis was about 70% of control values, whereas the incorporation of [14C]propionate into plasma glucose was 94% of control values. In contrast, the incorporation of other glucose precursors into glucose was decreased 30–50% by insulin. Therefore, insulin does not appear to decrease the utilization of propionate in gluconeogenesis. These results are consistent with the proposition that insulin differentially affects the rate of incorporation of glucose precursors into glucose in ruminant animals.

Keywords

InsulinPropionate metabolismGluconeogenesisSheep
Type
Hormones and Metabolism
Information
British Journal of Nutrition , Volume 64 , Issue 1 , July 1990 , pp. 95 - 101
Copyright
Copyright © The Nutrition Society 1990

References

Baird, G. D., Lomax, M. A., Symonds, H. W. & Shaw, S. R. (1980). Net hepatic and splanchnic metabolism of lactate, pyruvate and propionate in dairy cows in vitro in relation to lactation and nutrient supply. Biochemical Journal 186, 47–58.CrossRefGoogle Scholar
Bergman, E. N. (1973). Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Cornell Veterinarian 63, 341–382.Google Scholar
Bergman, E. N., Katz, M. L. & Kaufman, C. F. (1970). Quantitative aspects of hepatic and portal glucose metabolism and turnover in sheep. American Journal of Physiology 219, 785–793.CrossRefGoogle ScholarPubMed
Bergman, E. N., Roe, W. E. & Kon, K. (1966). Quantitative aspects of propionate metabolism and gluconeogenesis in sheep. American Journal of Physiology 211, 793–799.CrossRefGoogle ScholarPubMed
Bergman, E. N. & Wolff, J. E. (1971). Metabolism of volatile fatty acids by liver and portal-drained viscera in sheep. American Journal of Physiology 221, 586–592.CrossRefGoogle ScholarPubMed
Bessman, S. P., Mohan, C. & Zaidise, I. (1986). Intracellular site of insulin action: mitochondrial Krebs cycle. Proceedings of the National Academy of Sciences, USA 83, 5067–5070.CrossRefGoogle ScholarPubMed
Bishop, J. S., Steele, R., Altszuler, N., Dunn, A., Bjerknes, C. & DeBodo, R. C. (1965). Effects of insulin on liver glycogen synthesis and break-down in the dog. American Journal of Physiology 208, 307–316.CrossRefGoogle Scholar
Blair, A. & Segal, S. (1960). The isolation of blood glucose as potassium gluconate. Journal of Laboratory and Clinical Medicine 55, 959–964.Google ScholarPubMed
Brockman, R. P. (1979). Glucagon responses to exercise in sheep. Australian Journal of Biological Sciences 32, 215–220.CrossRefGoogle ScholarPubMed
Brockman, R. P. (1982). Insulin and glucagon responses in plasma to intraportal infusions of propionate and butyrate in sheep (Ovis aries). Comparative Biochemistry and Physiology 73A, 237–238.CrossRefGoogle Scholar
Brockman, R. P. (1984). Validation of an equation for calculation of glucose appearance during nonsteady state in sheep. Canadian Journal of Physiology and Pharmacology 62, 341–344.CrossRefGoogle ScholarPubMed
Brockman, R. P. (1985). Role of insulin in regulating hepatic gluconeogenesis in sheep. Canadian Journal of Physiology and Pharmacology 63, 1460–1464.CrossRefGoogle ScholarPubMed
Brockman, R. P. & Greer, C. (1980). Effects of somatostatin and glucagon on the utilization of [2-14C]propionate in glucose production in sheep. Australian Journal of Biological Sciences 33, 457–464.CrossRefGoogle ScholarPubMed
Brockman, R. P. & Laarveld, B. (1986). Effect of insulin on gluconeogenesis and the metabolism of lactate in sheep. Canadian Journal of Physiology and Pharmacology 64, 1055–1059.CrossRefGoogle Scholar
Brockman, R. P. & Manns, J. G. (1974). Effects of glucagon on activities of hepatic enzymes in sheep. Cornell Veterinarian 64, 217–224.Google ScholarPubMed
Brockman, R. P. & Manns, J. G. (1985). Characterization of antiserum 26GP for radioimmunoassay of glucagon in sheep plasma. Canadian Journal of Animal Science 65, 765–768.CrossRefGoogle Scholar
Canadian Council on Animal Care (1984). Guide to the Care and Use of Experimental Animals, 2 vols. Ottawa, Ontario: CCAC.Google Scholar
DeBodo, R. C., Steele, R., Altszuler, N. A., Dunn, A. & Bishop, J. S. (1964). On hormonal regulation of carbohydrate metabolism: studies with 14C-glucose. Recent Progress in Hormone Research 19, 445–482.Google Scholar
Filsell, O. H., Jarrett, I. G., Taylor, P. H. & Keech, D. B. (1969). Effects of fasting, diabetes and glucocorticoids on gluconeogenic enzymes in the sheep. Biochimica Biophysica Acta 184, 54–63.CrossRefGoogle ScholarPubMed
Judson, J. G. & Leng, R. A. (1973). Studies on the control of gluconeogenesis in sheep: effect of propionate, casein and butyrate infusions. British Journal of Nutrition 29, 175–195.CrossRefGoogle ScholarPubMed
Katz, M. L. & Bergman, E. N. (1969 a). A method of simultaneous cannulation of the major splanchnic blood vessels of the sheep. American Journal of Veterinary Research 30, 655–661.Google ScholarPubMed
Katz, M. L. & Bergman, E. N. (1969 b). Hepatic and portal metabolism of glucose, free fatty acids and ketone bodies in the sheep. American Journal of Physiology 216, 953–960.CrossRefGoogle ScholarPubMed
Leng, R. A. (1970). Glucose synthesis in ruminants. Advances in Veterinary Science 14, 209–260.Google ScholarPubMed
Leng, R. A. & Annison, E. F. (1963). Metabolism of acetate, propionate and butyrate by sheep-liver slices. Biochemical Journal 86, 319–327.CrossRefGoogle ScholarPubMed
Li, J. C. R. (1964). Statistical Inference, vol. 1. Ann Arbor, MI: Edwards Brothers Inc.Google Scholar
Lomax, M. A. & Baird, G. D. (1983). Blood flow and nutrient exchange across the liver and gut of the dairy cow. British Journal of Nutrition 49, 481–496.CrossRefGoogle ScholarPubMed
Ravussin, E. & Bogardus, C. (1982). Thermogenic response to insulin and glucose in man: a model to evaluate the different components of the thermic effect of carbohydrate. Life Sciences 31, 2011–2018.CrossRefGoogle Scholar
Soling, H. D. & Seuffert, C. D. (1975). Direct in vitro effects of insulin on liver metabolism. In Handbook of Physiology of Insulin, part 2, pp. 413–433. [Hasselblatt, A. and Bruchhausen, F. ν, editors]. New York: Springer-Verlag.Google Scholar
White, A. L., Handler, P., Hill, R. L. & Lekman, I. R. (1978). Lipid Metabolism I. In Principles of Biochemistry, 6th ed, pp. 568–606. New York: McGraw-Hill.Google Scholar