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The effect of high intakes of casein and casein phosphopeptide on calcium absorption in the rat

Published online by Cambridge University Press:  09 March 2007

Teresa Bennett ,
Audrey Desmond ,
Maria Harrington ,
David McDonagh ,
Richard FitzGerald ,
Albert Flynn  and
Kevin D. Cashman
Teresa Bennett
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Audrey Desmond
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Maria Harrington
Affiliation:
Department of Nutrition, University College, Cork, Ireland
David McDonagh
Affiliation:
Dairy Quality Department, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
Richard FitzGerald
Affiliation:
Life Science Department, University of Limerick, Limerick, Ireland
Albert Flynn
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Kevin D. Cashman*
Affiliation:
Department of Nutrition, University College, Cork, Ireland
*
*Corresponding author: Dr Kevin Cashman, fax +353 21 270244, email [email protected]
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Abstract

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The effect of the level or source of dietary protein or protein-derived peptides on Ca absorption is not well understood. We determined, therefore, the influence of habitual dietary casein level, meal casein and meal casein phosphopeptide (CPP) on Ca absorption in the rat. True fractional Ca absorption was investigated in male 7-week-old rats, Wistar strain, in three separate studies using a faecal 47Sc : 47Ca ratio method. In studies A and C, rats (n 8 per group) were fed on a purified diet containing 200 g casein/kg for 2 weeks. Rats were then given a 47Ca-labelled meal (10 g) containing (per kg) either 0, 100, 200, or 300 g casein (study A) or 0, 100, 200, 350 or 500 g CPP (study C). In study B, rats (n 24 per group) were fed on a purified diet containing (per kg) either 200, 350 or 500 g casein for 2 weeks. Each group was then further randomized into three groups (n 8 per group) and given a 47Ca-labelled meal (10 g of the same diet) containing (per kg) either 200, 350 or 500 g casein. Ca absorption from a meal was unaffected by increasing meal casein concentration from 0 to 300 g/kg (study A), but was increased with a meal casein content of 500 g/kg (study B). Fractional Ca absorption decreased with increasing usual dietary casein intake in the range 200–500 g/kg (study B), suggesting intestinal adaptation. Ca absorption was unaffected by inclusion of 100 g CPP/kg in a single meal but was significantly (P < 0·001) reduced by 200, 350 and 500 g CPP/kg meal, with no evident dose-relationship. Thus, while Ca absorption was enhanced by high-casein meals, the mechanism remains unclear.

Keywords

Calcium absorptionCaseinCasein phosphopeptide
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 6 , June 2000 , pp. 673 - 680
Copyright
Copyright © The Nutrition Society 2000

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