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Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function

Published online by Cambridge University Press:  09 March 2007

Nicole Gottstein ,
Benjamin A. Ewins ,
Clair Eccleston ,
Gary P. Hubbard ,
Ian C. Kavanagh ,
Anne-Marie Minihane ,
Peter D. Weinberg  and
Gerald Rimbach
Nicole Gottstein
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Benjamin A. Ewins
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Clair Eccleston
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Gary P. Hubbard
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Ian C. Kavanagh
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Anne-Marie Minihane
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
Peter D. Weinberg
Affiliation:
School of Animal and Microbial Sciences, The University of Reading, Reading, UK
Gerald Rimbach*
Affiliation:
Hugh Sinclair Human Nutrition Unit, School of Food Biosciences, University of Reading, UK
*
*Corresponding Author: Dr Gerald Rimbach, fax +44 118 931 0080, email [email protected]
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Abstract

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There has been much recent interest in the cardiovascular benefits of dietary isoflavones. The aim of the present in vitro studies was to investigate potential anti-thrombogenic and anti-atherogenic effects of the isoflavones genistein and daidzein in platelets, macrophages and endothelial cells. Pre-treatment with either isoflavone inhibited collagen-induced platelet aggregation in a dose-dependent manner. In a macrophage cell line (RAW 264·7) activated with interferon γ plus lipopolysaccharide, both isoflavones were found to inhibit NO production and tumour necrosis factor α (TNF-α) secretion dose-dependently, but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo-oxygenase-2. Both isoflavones also dose-dependently decreased monocyte chemoattractant protein-1 secretion induced by TNF-α in human umbilical vein endothelial cells. Compared with daidzein, genistein exerted greater inhibitory effects for all parameters studied. The present data contributes to our knowledge on the molecular mechanisms by which isoflavones may protect against coronary artery disease. Further studies are required to determine whether the effects of isoflavones observed in the current in vitro studies are relevant to the aetiology of coronary artery disease in vivo.

Keywords

GenisteinDaidzeinPlatelet aggregationArteriosclerosis
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 5 , May 2003 , pp. 607 - 615
Copyright
Copyright © The Nutrition Society 2003

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