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Effect of dietary quercetin on oxidative DNA damage in healthy human subjects

Published online by Cambridge University Press:  09 March 2007

Emily R. Beatty ,
James D. O'Reilly ,
Timothy G. England ,
Gareth T. McAnlis ,
Ian S. Young ,
Barry Halliwell ,
Catherine A. Geissler ,
Tom A. B. Sanders  and
Helen Wiseman
Emily R. Beatty
Affiliation:
Nutrition, Food & Health Research Centre, Department of Nutrition and Dietetics, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
James D. O'Reilly
Affiliation:
Nutrition, Food & Health Research Centre, Department of Nutrition and Dietetics, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
Timothy G. England
Affiliation:
Wolfson Centre for Age-Related Diseases, Guy's, King's and St Thomas' School of Medicine, Guy's Campus, London SE1 8RT, UK
Gareth T. McAnlis
Affiliation:
Department of Clinical Biochemistry, The Queen's University of Belfast, Institute of Clinical Science, Royal Victoria Hospital, Grosvenor Road, Belfast B12 6BJ, Northern Ireland, UK
Ian S. Young
Affiliation:
Department of Clinical Biochemistry, The Queen's University of Belfast, Institute of Clinical Science, Royal Victoria Hospital, Grosvenor Road, Belfast B12 6BJ, Northern Ireland, UK
Barry Halliwell
Affiliation:
Wolfson Centre for Age-Related Diseases, Guy's, King's and St Thomas' School of Medicine, Guy's Campus, London SE1 8RT, UK Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
Catherine A. Geissler
Affiliation:
Nutrition, Food & Health Research Centre, Department of Nutrition and Dietetics, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
Tom A. B. Sanders
Affiliation:
Nutrition, Food & Health Research Centre, Department of Nutrition and Dietetics, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
Helen Wiseman*
Affiliation:
Nutrition, Food & Health Research Centre, Department of Nutrition and Dietetics, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
*
*Corresponding author: Dr Helen Wiseman, fax +44 (0)20 7848 4185, email [email protected]
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Abstract

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The effect of dietary intake of flavonols (predominantly quercetin) on oxidative DNA damage was studied in thirty-six healthy human subjects (sixteen men, twenty women). The study was a randomised crossover study, comprising two 14 d treatments of either a low-flavonol (LF) or high-flavonol (HF) diet with a 14 d wash-out period between treatments. Subjects were asked to avoid foods containing flavonols, flavones and flavanols during the LF dietary treatment period and to consume one 150 g onion (Allium cepa) cake (containing 89·7 mg quercetin) and one 300 ml cup of black tea (containing 1·4 mg quercetin) daily during the HF dietary treatment. A 7 d food diary was kept during each dietary period and blood samples were taken after each dietary treatment. Products of oxidative damage to DNA bases were measured in DNA from leucocytes. The study had more than 95 % power to detect a change of 20 % in DNA damage products Plasma vitamin C and plasma quercetin concentrations were also measured. No significant differences in intake of macronutrients or assessed micronutrients, measured DNA base damage products, or plasma vitamin C were found between the HF and LF dietary treatments. The plasma quercetin concentration was significantly higher after the HF dietary treatment period (228·5 (SEM 34·7) nmol/l) than after the LF dietary treatment period (less than the limit of detection, i.e. <66·2 nmol/l). These findings do not support the hypothesis that dietary quercetin intake substantially affects oxidative DNA damage in leucocytes.

Keywords

FlavonolsQuercetinOnionsBlack teaOxidative DNA damage
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 6 , December 2000 , pp. 919 - 925
Copyright
Copyright © The Nutrition Society 2000

References

Aziz, AA, Edwards, CA, Lean, MEJ & Crozier, A (1998) Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions. Free Radical Research 29, 257–269.CrossRefGoogle ScholarPubMed
Beatty, ER, England, TG, Geissler, CA, Aruoma, OI & Halliwell, B (1999) Effects of antioxidant vitamin supplementation on markers of DNA damage and plasma antioxidants. Proceedings of the Nutrition Society 58, 44A.Google Scholar
Bingham, SA, Atkinson, C, Liggins, J, Bluck, L & Coward, A (1998) Plant oestrogens: where are we now. British Journal of Nutrition 79, 393–406.CrossRefGoogle ScholarPubMed
Bingham, SA, Cassidy, A, Cole, TJ, Welch, A, Runswick, SA, Black, AE, Thurnham, D, Bates, C, Khaw, KT, Key, TJA & Day, NE (1995) Validation of weighed records and other methods of dietary assessment using the 24 h urine nitrogen technique and other biological markers. British Journal of Nutrition 73, 531–550.Google Scholar
Boeing, H, Jedrychowski, W, Wahrendorf, J, Popiela, T, Tobiaszadamczyk, B and Kulig, A (1991) Dietary risk-factors in intestinal and diffuse types of stomach-cancer – a multicenter case-control study in Poland. Cancer Causes and Control 2, 227–233.Google Scholar
Cai, Q, Rahn, RO & Zhang, R (1997) Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Letters 119, 99–107.Google Scholar
Carbonneau, MA, Leger, CL, Descomps, B, Michel, F & Monnier, L (1998) Improvement in the antioxidant status of plasma and low-density lipoprotein in subjects receiving a red wine phenolics mixture. Journal of the American Oil Chemists Society 75, 235–240.CrossRefGoogle Scholar
Cook, NC & Samman, S (1996) Flavonoids: chemistry, metabolism, cardioprotective effects and dietary sources. Journal of Nutritional Biochemistry 7, 66–76.CrossRefGoogle Scholar
Craft, NE, Brown, ED & Smith, JCJ (1988) Effects of storage and handling conditions on concentrations of individual carotenoids, retinol and tocopherol in plasma. Clinical Chemistry 34, 44–48.Google Scholar
Crozier, A, Lean, MEJ, McDonald, MS & Black, C (1997) Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce and celery. Journal of Agricultural and Food Chemistry 45, 590–595.Google Scholar
de Vries, JHM, Hollman, PCH, Mayboom, S, Buysman, MNCP, Zock, PL, van Staveren, WA & Katan, MB (1998) Plasma concentrations and urinary excretion of the antioxidant flavonols quercetin and kaempferol as biomarkers for dietary intake. American Journal of Clinical Nutrition 68, 60–65.Google Scholar
de Whalley, CV, Rankin, SM, Hoult, JRS, Jessup, W & Leake, DS (1990) Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages. Biochemical Pharmacology 39, 1743–1750.Google Scholar
Duthie, SJ, Collins, AR, Duthie, GG & Dobson, VL (1997) Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes. Mutation Research 393, 223–231.Google Scholar
Duthie, SJ, Johnson, W & Dobson, VL (1997) The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimidines) and growth in human cells. Mutation Research 390, 141–151.Google Scholar
England, TG, Jenner, A, Aruoma, OI & Halliwell, B (1998) Determination of oxidative DNA base damage by gas chromatography-mass spectrometry. Effect of derivatisation conditions on artifactual formation of certain base oxidation products. Free Radical Research 29, 321–330.Google Scholar
Fenech, M, Stockley, C & Aitken, C (1997) Moderate wine consumption protects against hydrogen peroxide-induced DNA damage. Mutagenesis 12, 289–296.Google Scholar
Goldberg, GR, Black, AE, Jebb, SA, Cole, TJ, Murgatroyd, PR, Coward, WA & Prentice, AM (1991) Critical evaluation of energy intake data using fundamental principles of energy physiology. 1. Derivation of cut-off limits to identify under-recording. European Journal of Clinical Nutrition 45, 569–581.Google ScholarPubMed
Hertog, MGL, Bueno de Mesquita, HB, Fehily, AM, Sweetnam, PM, Elwood, PC & Kromhout, D (1996) Fruit and vegetable consumption and cancer mortality in the Caerphilly Study. Cancer Epidemiology, Biomarkers and Prevention 5, 673–677.Google ScholarPubMed
Hertog, MGL, Feskens, EJM, Hollman, PCH, Katan, MB & Kromhout, D (1993) Dietary antioxidant flavonoids and the risk of coronary heart disease: the Zutphen Elderly Study. Lancet 342, 1007–1011.Google Scholar
Hertog, MGL, Feskens, EJM, Hollman, PCH, Katan, MB & Kromhout, D (1994) Dietary flavonoids and cancer risk in the Zutphen Elderly Study. Nutrition and Cancer 22, 175–184.Google Scholar
Hertog, MGL & Hollman, PCH (1996) Potential health effects of the dietary flavonol quercetin. European Journal of Clinical Nutrition 50, 63–71.Google Scholar
Hertog, MGL, Hollman, PCH & Katan, MB (1992) Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. Journal of Agricultural and Food Chemistry 40, 2379–2383.Google Scholar
Hertog, MGL, Hollman, PCH, Katan, MB & Kromhout, D (1993 b) Intake of potentially anticarcinogenic flavonoids and their determinants in adults in the Netherlands. Nutrition and Cancer 20, 21–29.Google Scholar
Hertog, MGL, Hollman, PCH & Van de Putte, B (1993 c) Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juices. Journal of Agricultural and Food Chemistry 41, 1242–1246.CrossRefGoogle Scholar
Hertog, MGL, Kromhout, D, Aravanis, C, Blackburn, H, Buzina, R, Fidanza, F, Giampaoli, S, Jansen, A, Menotti, A, Nedeljkovic, S, Pekkarinen, M, Simic, BS, Toshima, H, Feskens, EJM, Hollman, PCH & Katan, MB (1995) Flavonoid intake and the long-term risk of coronary heart disease and cancer in the Seven Countries Study. Archives of Internal Medicine 155, 381–386.Google Scholar
Hollman, PCH (1997) Bioavailability of flavonoids. European Journal of Clinical Nutrition 51 Suppl. 1, S66–S69.Google Scholar
Hollman, PCH & Katan, MB (1997) Absorption, metabolism and health effects of dietary flavonoids in man. Biomedicine and Pharmacotherapy 51, 305–310.Google Scholar
Hollman, PCH, van Trijp, JMP & Buysman, MNCP (1996) Fluorescence detection of flavonols in HPLC by postcolumn chelation with aluminium. Analytical Chemistry 68, 3511–3515.CrossRefGoogle Scholar
Hollman, PCH, van Trijp, JMP, Mengelers, MJB, de Vries, JHM & Katan, MB (1997) Bioavailability of the dietary antioxidant flavonol quercetin in man. Cancer Letters 114, 139–140.CrossRefGoogle ScholarPubMed
Ishikawa, T, Suzukawa, M, Ito, T, Yoshida, H, Ayaori, M, Nishiwaki, M, Yonemura, A, Hara, Y & Nakamura, H (1997) Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. American Journal of Clinical Nutrition 66, 261–266.CrossRefGoogle ScholarPubMed
Jenner, A, England, TG, Aruoma, OI & Halliwell, B (1998) Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: ethanethiol prevents artifactual generation of oxidized DNA bases. Biochemical Journal 331, 365–369.Google Scholar
Justesen, U, Knuthsen, P & Leth, T (1997) Determination of plant polyphenols in Danish foodstuffs by HPLC-UV and LC-MS detection. Cancer Letters 114, 165–167.CrossRefGoogle ScholarPubMed
Key, T, Oakes, S, Davey, G, Moore, J, Edmond, LM, McLoone, UJ & Thurnham, DI (1996) Stability of vitamins A, C, & E, carotenoids, lipids and testosterone in whole blood stored at 4°C for 6 and 24 hours before separation of serum and plasma. Cancer Epidemiology, Biomarkers and Prevention 5, 811–814.Google Scholar
Lean, ME, Noroozi, M, Kelly, I, Burns, J, Talwar, D, Sattar, N & Crozier, A (1999) Dietary flavonols protect diabetic human lymphocytes against oxidative damage to DNA. Diabetes 48, 176–181.Google Scholar
McAnlis, GT, McEneny, J, Pearce, J & Young, IS (1999) Absorption and antioxidant effects of quercetin from onions in man. European Journal of Clinical Nutrition 53, 92–96.Google Scholar
McAnlis, GT, Pearce, J & Young, IS (1998) Black tea does not protect low density lipoprotein from oxidative modification. European Journal of Clinical Nutrition 52, 202–206.Google Scholar
McDonald, MS, Hughes, M, Burns, J, Lean, MEJ, Matthews, D & Crozier, A (1998) Survey of the free and conjugated myricetin and quercetin content of red wines of different geographical origins. Journal of Agricultural and Food Chemistry 46, 368–375.Google Scholar
Nigdikar, SV, Williams, NR, Griffin, BA & Howard, AN (1998) Consumption of red wine polyphenols reduces the susceptibility of low-density lipoproteins to oxidation in vivo. American Journal of Clinical Nutrition 68, 258–265.Google Scholar
Noroozi, M, Angerson, WJ & Lean, MEJ (1998) Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. American Journal of Clinical Nutrition 67, 1210–1218.Google Scholar
O'Reilly, J, Pollard, L, Leake, D, Sanders, TAB & Wiseman, H (1997) Quercetin is a potent inhibitor of oxidative damage to human LDL isolated from fresh and frozen plasma. Proceedings of the Nutrition Society 56, 287A.Google Scholar
Podmore, ID, Griffiths, HR, Herbert, KE, Mistry, N, Mistry, P & Lunec, J (1998) Vitamin C exhibits pro-oxidant properties. Nature 392, 559.Google Scholar
Rehman, A, Jenner, A & Halliwell, B (2000) Gas chromatography-mass spectometry analysis of DNA: optimization of protocols for isolation and analysis of DNA from human blood Methods in Enzymology(In the Press).Google Scholar
Rehman, A, Nourooz-Zadeh, J, Moller, W, Tritschler, H, Pereira, P & Halliwell, B (1999) Increased oxidative damage to all DNA bases in patients with type II diabetes mellitus. FEBS Letters 448, 120–122.CrossRefGoogle ScholarPubMed
Reinli, K & Block, G (1996) Phytoestrogen content of foods – a compendium of literature values. Nutrition and Cancer 26, 123–148.Google Scholar
Rice-Evans, CA, Miller, NJ & Paganga, G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine 20, 933–956.Google Scholar
Rimm, ER, Katan, MB, Ascherio, A, Stampfer, M & Willet, W (1996) Relation between intake of flavonoids and risk for coronary heart disease in male health professionals. Annals of Internal Medicine 125, 384–389.Google Scholar
Sankaranarayanan, R, Varghese, C, Duffy, SW, Padmakumary, G, Day, NE & Nair, MK (1994) A case-control study of diet and lung-cancer in Kerala, South India. International Journal of Cancer 58, 644–649.Google Scholar
Steinmetz, KA & Potter, JD (1993) Food-group consumption and colon cancer in the Adelaide case-control study. 1. Vegetables and fruit. International Journal of Cancer 53, 711–719.Google Scholar
Uda, Y, Price, KR, Williamson, G & Rhodes, MJC (1997) Induction of the anticarcinogenic marker enzyme, quinone reductase, in human hepatoma cells in vitro by flavonoids. Cancer Letters 120, 213–216.Google Scholar
Verma, AK, Johnson, JA, Gould, MN & Tanner, MA (1988) Inhibition of 7,12-dimethlylbenz(a)anthracene and N-nitrosomethylurea-induced rat mammary cancer by dietary flavonol quercetin. Cancer Research 48, 5754–5758.Google Scholar
Vuilleumier, JP & Keck, E (1989) Fluorometric assay of vitamin C in biological materials using a centrifugal analyser with fluorescence attachment. Journal of Micronutrient Analysis 5, 25–34.Google Scholar
Wiseman, H, (1998) Phytochemicals (b) Epidemiological Factors. In Encyclopedia of Human Nutrition, pp. 1549–1561 [Sadler, M, Caballero, B and Strain, S, editors]. London: Academic Press.Google Scholar
Wiseman, H (1999) The bioavailability of non-nutrient plant factors: dietary flavonoids and phyto-oestrogens. Proceedings of the Nutrition Society 58, 139–146.Google Scholar
Wiseman, H, O'Reilly, J, Lim, P, Garnett, AP, Huang, W-C, Sanders, TAB, (1998) Antioxidant properties of the isoflavone phytoestrogen functional ingredient in soya products. In Functional Foods, the Consumer, the Products and the Evidence, 80–86 [Sadler, M and Saltmarsh, M, editors]. Cambridge: Royal Society of Chemistry.Google Scholar