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Effect of dietary fats on the δ6- and δ5-desaturation of fatty acids in rat liver microsomes

Published online by Cambridge University Press:  24 July 2007

D. Kirstein ,
C.-E. Høy  and
G. Hølmer
D. Kirstein
Affiliation:
Department of Biochemistry and Nutrition, The Technical University of Denmark, Building 224, DK-2800 Lyngby, Denmark
C.-E. Høy
Affiliation:
Department of Biochemistry and Nutrition, The Technical University of Denmark, Building 224, DK-2800 Lyngby, Denmark
G. Hølmer
Affiliation:
Department of Biochemistry and Nutrition, The Technical University of Denmark, Building 224, DK-2800 Lyngby, Denmark
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Abstract

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1. Rats were given diets containing (% dietary energy): 46 arachis oil (AO), 36 partially-hydrogenated arachis oil (HAO) + 10 AO, 36 partially-hydrogenated marine oil (HMO) + 10 AO, or 46 of a combination of rape-seed oils high and low in erucic acid (RSO + LERSO).

2. In the liver microsomes the content of arachidonic acid (20:4ω6) was reduced inthe groups given HAO + AO and HMO + AO.

3. The rates of Δ6-desaturation of linoleic acid into γ-linolenic acid (18:3ω6) and of Δ5-desaturation of dihomo-γ-linolenic acid into arachidonic acid were studied in vitro at two substrate levels: a high substrate level reflecting maximal microsomal desaturase activity in rat liver and a low substrate level reflecting desaturase activity under physiological conditions.

4. Dietary HAO, rich in 18:1 isomers, suppressed the Δ6-desaturase activity butnot the Δ5-desaturase activity. Dietary HMO, rich in 18:1, 20:1 and 22:1 isomers, reduced both Δ6- and Δ5-desaturase activities.

Type
Research Article
Information
British Journal of Nutrition , Volume 50 , Issue 3 , November 1983 , pp. 749 - 756
Copyright
Copyright © The Nutrition Society 1983

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