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The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

Published online by Cambridge University Press:  09 March 2007

A. Donkoh  and
P. J. Moughan
A. Donkoh
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
P. J. Moughan
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
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Abstract

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The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

Keywords

Ileal digestibilityProteinMeat-and-bone mealRat
Type
Dietary protein effects on protein turnover
Information
British Journal of Nutrition , Volume 72 , Issue 1 , July 1994 , pp. 59 - 68
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Alpers, D. H. (1987). Digestion and absorption of carbohydrates and proteins. In Physiology ofthe Gastrointestinal Tract, 2nd ed., vol. 2, pp. 1469–1487 [Johnson, L. R., editor] New York: Raven Press.Google Scholar
Association of Official Analytical Chemists (1980). Oficial Methods of Analysis, 13th ed. Washington, DC: AOAC.Google Scholar
Bell, J. M. & Keith, M. O. (1989). Factors affecting the digestibility by pigs of energy and protein in wheat, barley and sorghum diets supplemented with canola meal. Animal Feed Science and Technology 24, 253–265.CrossRefGoogle Scholar
Bell, J. M., Shires, A. & Keith, M. O. (1983). Effect of hull and protein contents of barley on protein and energy digestibility and feeding value for pigs. Canadian Journal of Animal Science 63, 201–211.CrossRefGoogle Scholar
Bressani, R., Torun, B., Elias, L. G., Navrrete, D. A. & Vargas, E. (1981). A short-term procedure to evaluate protein quality in young and adult human subjects. In Protein Quality in Humans: Assessment and In Vitro Estimation, pp. 98–117 [Bodwell, C. E., Adkins, J. D., and Hopkins, D. T., editor] Westport, CT: AVI Publishing Co.Google Scholar
Buraczewska, L. & Horaczynski, H. (1983). Influence of dry matter intake on ileal nitrogen output and of protein intake on digestibility of amino acids in pigs. In Protein Metabolism and Nutrition. Les Colloques de l'INRA, no. 16, pp. 381–384 [Pion, R.M., Arnal and Bonin, D., editors]. France: Clennont-Ferrand.Google Scholar
Buraczewski, S. (1980). Aspects of protein digestion and metabolism in monogastric animals. In Proceedings of the Third European Association for Animal Production Symposium on Protein Metabolism and Nutrition, pp. 179–195 [Oslage, H. J. and Rhor, K., editors]. Braunschweig, Germany: Institute of Animal Nutrition.Google Scholar
Butts, C. A., Moughan, P. J. & Smith, W. C. (1991). Endogenous amino acid flow at the terminal ileum of the rat determined under conditions of peptide alimentation. Journal of the Science of Food and Agriculture 55, 175–187.CrossRefGoogle Scholar
Costigan, P. & Ellis, K. J. (1987). Analysis of faecal chromium derived from controlled-release marker devices. New Zealand Journal of Technology 3, 89–92.Google Scholar
Dammers, J. (1964). Digestibility research in pig feeding. The influence of different factors on the digestion of feed components and the digestibility of amino acids. PhD Thesis, University of Leuven, Belgium.Google Scholar
den Hartog, L. A., Verstegen, M. W. A. & Huisman, J. (1989). Amino acid digestibility in pigs as affected by diet composition. In Absorption and Utilization of Amino Acids, pp. 201–216 [Friedman, M., editor]. Boca Raton, Florida: CRC Press.Google Scholar
Donkoh, A. (1993). Amino acid digestibility in meat and bone meal for the growing pig. The development of a digestibility assay based on the laboratory rat. PhD Thesis, Massey University, New Zealand.Google Scholar
Donkoh, A., Moughan, P. J. & Smith, W. C. (1994). The laboratory rat as a model animal for determining ileal amino acid digestibility in meat and bone meal for the growing pig. Animal Feed Science and Technology (In the Press).Google Scholar
Eggum, B. O. (1973). A Study of Certain Factors Influencing Protein Utilization in Rats and Pigs. Report of the National Institute of Animal Science, Copenhagen, no. 406. Copenhagen: National Institute of Animal Science.Google Scholar
Fauconneau, G. & Michel, M. C. (1970). The role of the gastrointestinal tract in the regulation of protein metabolism. In Mammalian Protein Metabolism, vol. 4, pp. 481–522 [Munro, H. N., editor]. New York: Academic Press.CrossRefGoogle Scholar
Furuya, S. & Kaji, Y. (1989). Estimation of the true ileal digestibility of amino acids and nitrogen from the apparent values for growing pigs. Animal Feed Science and Technology 26, 271–285.CrossRefGoogle Scholar
Green, S. (1987). Digestibilities of Amino Acids in Foodstuffs for Poultry and Pigs. Digestibility Report 8/87, pp. 2–8. France: Rhone Poulenc Nutrition Laboratories.Google Scholar
Hopkins, D. T. (1981). Effects of variation in protein digestibility. In Protein Quality in Humans: Assessment and In Vitro Estimation, pp. 169–193 [Bodwell, C. E., Adkins, J. S., and Hopkins, D. T., editors]. Westport, CT: AVI Publishing Co.Google Scholar
Just-Nielsen, A. (1958). Digestibility of amino acids in relation to digestibility of nitrogen in bacon pigs. In Royal Veterinary and Agricultural College Yearbook, 1968, pp. 79–88. Copenhagen: Royal Veterinary and Agricultural College.Google Scholar
Keith, M. O. & Bell, J. M. (1991). Composition and digestibility of canola press cake as a feedstuff for use in swine diets. Canadian Journal of Animal Science 72, 879–885.CrossRefGoogle Scholar
Low, A. G. (1982). Endogenous nitrogen evaluation from absorption studies. In: Physiologic Digestive Chez Le Porc, pp. 189–198 [J. PLaplace, D. T., Corring, T., and Rerat, A., editors]. Paris: Institute National de la Recherche Agronomique.Google Scholar
McNab, J. M. (1989). Measuring availability of amino acids from digestibility experiments. In Proceedings of the Seventh European Symposium on Poultry Nutrition9–21 JuneGirona, Spain, pp. 45–53. World's Poultry Science Association.Google Scholar
Moughan, P. J., Darragh, A. J., Smith, W. C. & Butts, C. A. (1990). Perchloric and trichloroacetic acids as precipitants of protein in endogenous ileal digesta from the rat. Journal of the Science of Food and Agriculture, 52, 13–21.CrossRefGoogle Scholar
Sarwar, G. & Peace, R. W. (1986). Comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats. Journal of Nutrition 116, 1172–1184.CrossRefGoogle ScholarPubMed
Sauer, W. C. (1976). Factors affecting amino acid availab es for cereal grains and their components for monogastric animals. PhD Thesis, University of Manitoba, Canada.Google Scholar
Sauer, W. C., Just, A., Jorgensen, H. H., Fekadu, M. & Eggum, B. O. (1980). The influence of diet composition on the apparent digestibility of crude protein and amino acids at the terminal ileum and overall in pigs. Acta Agriculturae Scandinavica 30, 449–459.CrossRefGoogle Scholar
Sauer, W. C & Ozimek, L. (1986). Digestibility of amino acids in swine: results and their practical applications. A review. Livestock Production Science 15, 367–388.CrossRefGoogle Scholar
Shah, N. O., Atallah, M. T., Mahoney, R. R. & Pellett, P. L. (1982). Effect of dietary fibre components on endogenous nitrogen excretion and protein utilization in growing rats. Journal of Nutrition 112, 658–666.CrossRefGoogle ScholarPubMed
Tanksley, T. D. & Knabe, D. A. (1984). Ileal digestibilities of amino acids in pig feeds and their use in formulating diets. In Recent Advances in Animal Nutrition, pp. 75–95 [Haresign, w. and Cole, D. J. A., editors]. London: Butterworths.Google Scholar
Taverner, M. R. (1979). Ileal availability for pigs of amino acids in cereal grains. PhD Thesis, University of New England, Australia.Google Scholar
Thomsen, L. (1981). Bowl for feeding powdered diets to rats. Laboratory Animals 15, 177–178.CrossRefGoogle ScholarPubMed
Van Leeuwen, P., Sauer, W. C., Huisman, J., van Weerden, E. J., van Kleef, D. & den Hartog, L. A. (1987). Methodological aspects for the determination of amino acid digestibilities in pigs fitted with ileo-caecal re- entrant cannulas. Journal of Animal Nutrition and Animal Physiology 58, 122–133.CrossRefGoogle Scholar
Zuprizal Larbier, M., Chagneau, A. M. & Lessire, M. (1991). Effect of protein intake on true digestibility of amino acids in rapeseed meals for adult roosters force fed with moistened feed. Animal Feed Science and Technology 34, 255–260.CrossRefGoogle Scholar