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The effect of dietary composition and of insulin on gluconeogenesis in rainbow trout (Salmo gairdneri)

Published online by Cambridge University Press:  09 March 2007

C. B. Cowey ,
M. De La Higuera  and
J. W. Adron
C. B. Cowey
Affiliation:
Institute of Marine Biochemistry, St Fitticks Road, Aberdeen AB1 3RA
M. De La Higuera
Affiliation:
Institute of Marine Biochemistry, St Fitticks Road, Aberdeen AB1 3RA
J. W. Adron
Affiliation:
Institute of Marine Biochemistry, St Fitticks Road, Aberdeen AB1 3RA
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Abstract

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1. The activities at 15° of three gluconeogenic enzymes, d-fructose-1,6-diphosphate, 1-phosphohydrolase (EC 3.1.3.11), pyruvate carboxylase (EC 6.4.1.1) and phosphoenolpyruvate carboxykinase (4.1.1.32), were determined in liver, kidney, gill and muscle of rainbow trout (Salmo gairdneri) given a commercial diet. The results indicated that liver and kidney are the main sites of gluconeogenesis.

2. Glucose formation from pyruvate was approximately 6 μmol/h per g wet weight at 15° in liver slices of trout given a commercial diet.

3. Glucose diffusion space in trout was measured by the dilution principle after intravascular injection of a trace dose of [U-14C]glucose. Glucose space was found to be 13.7% of the body-weight. Gluconeogenesis in vivo amounted to approximately 45 μmol/kg body-weight per h.

4. Intraperitoneally injected [U-14C]alanine was quickly converted to glucose. Maximal incorporation of alanine into glucose occurred 6 h after alanine administration.

5. Rainbow trout given a high-protein diet gained in weight significantly during a 4-week period. Those given a high-carbohydrate diet did not make a significant weight gain over the same period. Gluconeogenesis from alanine was markedly reduced in fish given the high-carbohydrate diet. There was no significant difference in gluconeogenesis from alanine in fish given a high-protein diet and fish which were fasted for 21 d.

6. Gluconeogenesis from alanine in trout was suppressed by intravenous injection of insulin. This effect was found both in trout given a high-protein diet and in fasted trout.

Type
Research Article
Information
British Journal of Nutrition , Volume 38 , Issue 3 , November 1977 , pp. 385 - 395
Copyright
Copyright © The Nutrition Society 1977

References

REFERENCES

Ballard, F. J. (1965). Comp. Biochem. Physiol. 14, 437.CrossRefGoogle Scholar
Chang, V. M. & Idler, D. R. (1960). Can. J. Biochem. Physiol. 38, 553.CrossRefGoogle Scholar
Cowey, C. B. (1975). Proc. Nutr. Soc. 34, 57.CrossRefGoogle Scholar
Cowey, C. B., Adron, J. W., Brown, D. A. & Shanks, A. M. (1975). Br. J. Nutr. 33, 219.CrossRefGoogle Scholar
Cowey, C. B., Knox, D., Walton, M. J. & Adron, J. W. (1977). Br. J. Nutr. 38, 463.CrossRefGoogle Scholar
Cowey, C. B., Pope, J. A., Adron, J. W. & Blair, A. (1972). Br. J. Nutr. 28, 447.CrossRefGoogle Scholar
Deodhar, A. D. & Mistry, S. P. (1969). Archs Biochem. Biophys. 131, 507.CrossRefGoogle Scholar
Driedzic, W. R. & Kicenuik, J. W. (1976). J. Fish Res. Bd Can. 33, 173.CrossRefGoogle Scholar
Friedmann, B., Goodman, E. H. & Weinhouse, S. (1965). J. biol. Chem. 240, 3729.CrossRefGoogle Scholar
Friedmann, B., Goodman, E. H. & Weinhouse, S. (1967). J. biol. Chem. 242, 3620.CrossRefGoogle Scholar
Hayashi, S. & Ooshiro, Z. (1975). Bull. Jap. Soc. Scient. Fish. 41, 201.CrossRefGoogle Scholar
Holmes, S. N. & Donaldson, E. M. (1969). In Fish Physiology, p. 1 [Hoar, W. S. and Randall, D. J., editors]. New York: Academic Press.Google Scholar
Inui, Y. & Ohshima, Y. (1966). Bull. Jap. Soc. Scient. Fish. 32, 492.CrossRefGoogle Scholar
Johnson, J. A. & Fusaro, R. M. (1966). Analyt. Biochem. 15, 140.CrossRefGoogle Scholar
Krebs, H. A., Dierks, C. & Gascoyne, T. (1964). Biochem. J. 93, 112.CrossRefGoogle Scholar
Larsson, A. & Lewander, K. (1973). Comp. Biochem. Physiol. 44A, 367.CrossRefGoogle Scholar
MacLeod, R. A., Jonas, R. E. E. & Roberts, E. (1963). Can. J. Biochem. Physiol. 41, 1971.CrossRefGoogle Scholar
Mazeaud, F. (1973). Recherches sur la régulation des acides gras libres plasmatiques et de la glycéruie chez les poissons. PhD Thesis, University of Paris.Google Scholar
Newsholme, E. A. & Crabtree, B. (1970). FEBS Lett. 7, 195.CrossRefGoogle Scholar
Newsholme, E. A. & Start, C. (1973). Regulation in Metabolism, London: John Wiley & Sons.Google Scholar
Palmer, T. N. & Ryman, B. E. (1972). J. Fish Biol. 4, 311.CrossRefGoogle Scholar
Pogson, C. I. & Smith, S. A. (1975). Biochem. J. 152, 401.CrossRefGoogle Scholar
Saggerson, E. D. (1974). Biochem. J. 140, 211.CrossRefGoogle Scholar
Sakaguchi, M. & Kawai, A. (1970). Bull. Jap. Soc. Scient. Fish 36, 783.CrossRefGoogle Scholar
Satia, B. P. (1974). Progve. Fish Cult. 36, 80.CrossRefGoogle Scholar
Singh, R. P. & Nose, T. (1967). Bull. Freshwat. Fish Res. Lab., Tokyo, 17, 21.Google Scholar
Somogyi, M. (1945). J. biol. Chem. 160, 69.CrossRefGoogle Scholar
Tashima, L. & Cahill, G. F. (1968). Gen. Comp. Endocrinol. 11, 262.CrossRefGoogle Scholar
Thorpe, A. & Ince, B. W. (1976). Gen. Comp. Endocrinol. 30, 332.CrossRefGoogle Scholar
Thorson, T. (1961). Biol. Bull. 120, 238.CrossRefGoogle Scholar