Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-19T12:38:29.699Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of condensed tannins in Lotus corniculatus on plasma metabolism of methionine, cystine and inorganic sulphate by sheep

Published online by Cambridge University Press:  09 March 2007

Yuxi Wang ,
G. C. Waghorn ,
T. N. Barry  and
I. D. Shelton
Yuxi Wang
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
G. C. Waghorn
Affiliation:
Ag Research Grasslands, Palmerston North, New Zealand
T. N. Barry
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
I. D. Shelton
Affiliation:
Ag Research Grasslands, Palmerston North, New Zealand
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Fresh Lotus corniculatus containing 27 g extractable condensed tannin (CT)/kg dry matter (DM) and 8 g bound CT/kg DM was fed at hourly intervals to sheep held in metabolism cages to study the effects of CT on nutrient digestion and on metabolism of methionine, cystine and inorganic sulphate in plasma. Polyethylene glycol (PEG) was continuously infused into the rumen of half the sheep to remove the effects of CT. Principal measurements in the two groups were plasma irreversible loss (IRL) rate and interconversions of methionine, cystine and inorganic sulphate using 35S labelling. CT in Lotus corniculatus had no effects on the apparent digestion of cellulose and minerals, slightly depressed DM, organic matter and hemicellulose digestion and markedly reduced the apparent digestion of N (P 0·01). The concentration of NH3 and molar proportions of iso-butyric acid, iso-valeric acid and n−valeric acid in rumen fluid were markedly increased by the PEG infusion (P 0·01), whereas total volatile fatty acid concentration and molar proportions of acetic acid, propionic acid and n−butyric acid were not affected. PEG infusion temporarily increased rumen protozoa numbers. CT greatly increased the IRL of plasma cystine (131·1 v. 7·0 μmol/min; P 0·05) and reduced IRL of plasma inorganic sulphate (36·8 v. 48·1 μmol/min; P 0·01) but had no effect on methionine IRL. CT increased transulphuration of methionine to cystine (4·37 v. 1·24 μmol/min; P 0·05), increased cystine entering the plasma from whole-body protein turnover plus absorption from the small intestine (9·34 v. 5·75 μmol/min; P 0·05) and increased cystine flux to body synthetic reactions (11·89 v. 5·41 μmol/min; P 0·05). CT had no effect on the proportion of methionine total flux transferred to sulphate (0·05 v. 0·06; P 0·05), reduced the proportion of methionine flux transferred to body synthetic reactions (0·68 v. 0·86) and markedly reduced the proportion of cystine flux transferred to sulphate (0·09 v. 0·27; P 0·01). It was concluded that CT in Lotus corniculatus reduced rumen protein degradation and markedly increased utilization of plasma cystine for body synthetic reactions.

Keywords

Condensed tanninsDigestibilitySulphur-containing amino acidsInorganic sulphateSheep
Type
Effects of condensed tannins on sulphur metabolism
Information
British Journal of Nutrition , Volume 72 , Issue 6 , December 1994 , pp. 923 - 935
Copyright
Copyright © The Nutrition Society 1994

References

RERERENCES

Ahmed, A. E., Smithard, R. & Ellis, M. (1991). Activities of enzymes of the pancreas, and the lumen and mucosa of the small intestine in growing broiler cockerels fed on tannin-containing diets. British Journal of Nutrition 65, 189–197.CrossRefGoogle ScholarPubMed
Barry, T. N. (1989).Condensed tannins: their role in ruminant protein and carbohydrate digestion and possible effects upon the rumen ecology. In The Role of Protozoa and Fungi in Ruminant Digestion, pp. 153–169 [Nolan, J. V., Leng, R. A. and Deneyer, D. I., editors]. Armidale: Pernambul Books.Google Scholar
Barry, T. N. & Duncan, S. J. (1984). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 1. Voluntary intake. British Journal of Nutrition 51, 484–491.Google Scholar
Barry, T. N. & Manley, T. R. (1984). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 2. Quantitative digestion of carbohydrate and protein. British Journal of Nutrition 51, 493–504.CrossRefGoogle Scholar
Barry, T. N., Manley, T. R. L, Duncan, S. J. (1986). The role of condensed tannins in the nutritional value of Lotus pedunculatus. 4. Sites of carbohydrate and protein digestion as infuenced by dietary reactive tannin concentration. British Journal of Nutrition. 55, 123–137.Google Scholar
Bigham, M. L., Sumner, R. M. W. & Elliott, K. H. (1978). Seasonal wool production of Romney, Coopworth, Perendale, Cheviot, and Corriedale wethers. New Zeuland Journal of Agricultural Research 21, 377–382.CrossRefGoogle Scholar
Chiquette, J., Cheng, K. J., Costerton, J. W. & Milligan, L. P. (1988). Effects of tannins on the digestibility of two isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.) using in vitro and in sacco techniques. Canadian Journal of Animal Science 68, 751–760.CrossRefGoogle Scholar
Cohen, S. A, Meys, M. & Tarvin, T. L. (1989). The Pico. Tag Method. A Manual of Advanced Techniques for Amino Acid Analysis. Milford, MA: Millipore Corporation.Google Scholar
Cousins, B. W., Tanksley, T. D., Knabe, D. A. & Zebrowska, T. (1981). Nutrient digestibility and performance of pigs fed sorghums varying in tannin concentration. Journal of Animal Science 53, 1524–1537.CrossRefGoogle ScholarPubMed
Donnelly, E. D. & Anthony, W. B. (1969). Relationship of tannin, dry matter digestibility and crude protein in Sericea lespedeza. Crop Science 9, 361–362.Google Scholar
Donnelly, E. D. & Anthony, W. B. (1970). Effect of genotype and tannin on dry matter digestibility in Sericea lespedeza. Crop Science 10, 200–202.Google Scholar
Douglas, G. B., Donkers, P., Foote, A. G. & Barry, T. N. (1993). Determination of extractable and bound condensed tannins in forage species. In Proceedings of the XVIIth International Grass1ands Conference, pp. 204–206 [Baker, M. J., Crush, J. R. and Humphreys, L. R., editors]. Palmerston North, NZ: Dunmore Press.Google Scholar
El-Shazly, K. (1952). Degradation of protein in the rumen of the sheep. 2. The action of rumen micro-organisms on amino acids. Biochemical Journal 51, 647–653.CrossRefGoogle Scholar
Foo, L. Y., Jones, W. T., Porter, L. J. & Williams, V. M. (1982). Proanthocyanidin polymers of fodder legumes. Phytochemistry 21, 933–935.Google Scholar
Hogan, J. P. (1975). Symposium: protein and amino acid nutrition in the high producing cow. Quantitative aspects of nitrogen utilization in ruminants. Journal of Dairy Science 58, 1164–1177.CrossRefGoogle Scholar
Horigome, T., Kumar, R. & Okamoto, K. (1988). Effects of condensed tannins prepared from leaves of fodder plants on digestive enzymes in vitro and in the intestine of rats. British Journal of Nutrition 60, 275–285.CrossRefGoogle ScholarPubMed
Johnson, C. M. & Nishita, H. (1952). Microestimation of sulphur in plant materials, soils and irrigation waters, Analytical Chemistry 24, 736–742.CrossRefGoogle Scholar
Jones, W. T., Broadhurst, R. B. & Lyttleton, J. W. (1976). The condensed tannins of pasture legume species. Phytochemistry 15, 1047–1049.Google Scholar
Jones, W. T. & Mangan, J. L. (1977). Complexes of the condensed tannins of Sainfoin (Onobrychis viciifolia Scop.) with fraction 1 leaf protein and with submaxillary mucoprotein, and their reversal by polyethylene glycol and pH. Journal of the Science of Food and Agriculture 28, 126–136.CrossRefGoogle Scholar
Lee, J., Harris, P. M., Sinclair, B. R. & Treloar, B. P. (1992). The effect of condensed tannin-containing diets on whole body amino acid utilization in Romney sheep: consequences for wool growth. Proceedings of the New Zealand Society of Animal Production 52, 243–245.Google Scholar
Longstaff, M. & McNabb, J. M. (1991 a). The inhibitory effects of hull polysaccharides and tannins of field beans (Vicia faba L.) on the digestion of amino acids, starch and lipid and on digestive enzyme activities in young chicks. British Journal of Nutrition 65, 199–216.CrossRefGoogle Scholar
Longstaff, M. A. & McNabb, J. M. (1991 b). The effect of concentration of tannin-rich bean hulls (Vicia faba L.) on activities of lipase (EC 3.1.I.3) and α-amylase (EC 3·2·1·1) in digesta and pancreas and on the digestion of lipid and starch by young chicks. British Journal of Nutrition 66, 139–147.CrossRefGoogle ScholarPubMed
McLeod, M. N. (1974). Plant tannins - their role in forage quality. Nutrition Abstracts and Reviews 44, 803–815.Google Scholar
McNabb, W. C., Waghorn, G. C., Barry, T. N. & Shelton, I. D. (1993). The effect of condensed tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. British Journal of Nutrition 70, 647–661.CrossRefGoogle ScholarPubMed
Price, M. L. & Butler, L. G. (1980). Tannins and Nutrition. Station Bulletin no. 272. Department of Biochemistry, Agricultural Experiment Station, Purdue University, West Lafayette, Indiana.Google Scholar
Pritchard, D. A., Stocks, D. C., O'Sullivan, B. M., Martin, P. R., Hurwood, I.S. & O'Rourke, P. K. (1988). The effect of polyethylene glycol (PEG) on wool growth and liveweight of sheep consuming a mulga (Acacia aneura) diet. Proceedings of the Australian Society of Animal Production 17, 290–293.Google Scholar
Reed, J. D., McDowell, R. E., Van Soest, P. J. & Horvath, P. J. (1982). Condensed tannins: a factor limiting the use of Cassava forages. Journal of the Science of Food and Agriculture 33, 213–220.CrossRefGoogle Scholar
Reis, P. J. (1965 a). Variation in the sulphur content of wool. In Biology of the Skin and Hair Growth, pp. 365–379 [Lyne, A. G. and Short, B. F., editors]. Sydney: Angus and Robertson.Google Scholar
Reis, P. J. (1965 b). The growth and composition of wool. III. Variation in the sulphur content of wool. Australian Journal of Biological Science 18, 671–679.Google Scholar
Reis, P. J. (1979). Effects of amino acids on the growth and properties of wool. In Physiological and Environmental Limitations to Wool Growth, pp. 223–242 [Black, J. L. and Reis, P. J., editors]. Armidale: University of New England Publishing Unit.Google Scholar
Tabatabai, M. A. & Bremner, J. M. (1970). An alkaline oxidation method for determination of total sulphur in soils. Soil Science Society of America Proceedings 34, 62–65.Google Scholar
Terrill, T. H., Douglas, G. B., Foote, A. G., Purchas, R. W., Wilson, G. F. & Barry, T. N. (1992 a). The effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal of Agricultural Science, Cambridge 119, 265–273.CrossRefGoogle Scholar
Terrill, T. H., Rowan, A. M., Douglas, G. B. & Barry, T. N. (1992 b). Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture 58, 321–329.CrossRefGoogle Scholar
Van Soest, P. J. (1983). Nutritional Ecology of the Ruminant. Corvallis, Oregon: O & B Books, Inc.Google Scholar
Waghorn, G. C. (1990). Effect of condensed tannin on protein digestion and nutritive value of fresh herbage. Proceedings of the Australian Society of Animal Production 18, 412–415.Google Scholar
Waghorn, G. C., John, A, Jones, W. T. & Shelton, I. D. (1987 a). Nutritive value of Lotus corniculatus L. containing low and medium concentrations of condensed tannins for sheep. Proceedings of the New Zealand Society of Animal Production 47, 25–30.Google Scholar
Waghorn, G. C., Ulyatt, M. J., John, A. & Fisher, M. T. (1987 b). The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus. British Journal of Nutrition 57, 115–126.Google Scholar
Yuste, P., Longstaff, M. & McCorquodale, C. (1992). The effects of proanthocyanidin-rich hulls and proanthocyanidin extracts from bean (Vicia faba L.) hulls on nutrient digestibility and digestive enzyme activities in young chicks. British Journal of Nutrition 67, 57–65.CrossRefGoogle ScholarPubMed