Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-20T10:37:19.189Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of carbohydrate and fat variation in euenergetic diets on postabsorptive free fatty acid release

Published online by Cambridge University Press:  09 March 2007

Peter H. Bisschop ,
Mariëtte T. Ackermans ,
Erik Endert ,
An F. C. Ruiter ,
Alfred J. Meijer ,
Folkert Kuipers ,
Hans P. Sauerwein  and
Johannes A. Romijn
Peter H. Bisschop*
Affiliation:
Departments of Endocrinology and Metabolism, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands Department of Endocrinology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
Mariëtte T. Ackermans
Affiliation:
Clinical Chemistry, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
Erik Endert
Affiliation:
Clinical Chemistry, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
An F. C. Ruiter
Affiliation:
Clinical Chemistry, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
Alfred J. Meijer
Affiliation:
Biochemistry, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
Folkert Kuipers
Affiliation:
Center for Liver, Digestive and Metabolic Diseases, Academic Hospital Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
Hans P. Sauerwein
Affiliation:
Departments of Endocrinology and Metabolism, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
Johannes A. Romijn
Affiliation:
Department of Endocrinology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
*
*Corresponding author:Dr Peter H. Bisschop, fax +31 20 6917682, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Diet composition and energy content modulate free fatty acid (FFA) release. The aim of this study was to evaluate the dose–response effects of euenergetic variations in dietary carbohydrate and fat content on postabsorptive FFA release. The rate of appearance (Ra) of palmitate was measured by infusion of [2,2-2H2]palmitate after an overnight fast in six healthy men on three separate occasions, i.e. after 7 d on euenergetic control, high-carbohydrate and high-fat diets. The protein content and composition was identical for each diet. Postabsorptive plasma fatty acid concentrations were not different between the high-carbohydrate and control diets (0·36 (SE 0·07) V. 0·43 (se 0·04) mmol/l), but were increased after the high-fat diet (0·75 (se 0·09) mmol/l, (P<0·01 compared with the other diets). Ra palmitate was not different between the high-carbohydrate and control diets (1·36 (se 0·20) v. 1·47 (se 0·15) μmol/kg per min). However, Ra palmitate was increased to 2·36 (se 0·26) μmol/kg per min after the high-fat diet (P<0·01 compared with the other diets). The fatty acid flux and whole-body fat oxidation were not affected by the high-carbohydrate diet compared with the control diet, but were increased by 67 and 47 % respectively, on the high-fat diet (P<0·01 compared with the other diets). A euenergetic high-fat diet results in increased postabsorptive FFA release and fat oxidation, whereas a euenergetic high-carbohydrate diet does not affect these variables of fat metabolism.

Keywords

Free fatty acid releaseHigh-fat dietHigh-carbohydrate dietPalmitate
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 6 , June 2002 , pp. 555 - 559
Copyright
Copyright © The Nutrition Society 2002

References

Bisschop, PH, de Metz, J, Ackermans, MT, Endert, E, Pijl, H, Kuipers, F, Meijer, AJ, Sauerwein, HP & Romijn, JA (2001) Dietary fat content alters insulin-mediated glucose metabolism in healthy males. American Journal of Clinical Nutrition 73, 554–559.CrossRefGoogle Scholar
Eriksson, JW, Smith, U, Waagstein, F, Wysocki, M & Jansson, PA (1999) Glucose turnover and adipose tissue lipolysis are insulin-resistant in healthy relatives of type 2 diabetes patients: is cellular insulin resistance a secondary phenomenon? Diabetes 48, 1572–1578.CrossRefGoogle ScholarPubMed
Frayn, KN (1983) Calculation of substrate oxidation rates in vivo from gaseous exchange. Journal of Applied Physiology: Respiratory Environmental and Exercise Physiology 55, 628–634.CrossRefGoogle ScholarPubMed
Ingalls, ST, Kriaris, MS, Xu, Y, DeWulf, DW, Tserng, KY & Hoppel, CL (1993) Method for isolation of non-esterified fatty acids and several other classes of plasma lipids by column chromatography on silica gel. Journal of Chromatography B 619, 9–19.CrossRefGoogle ScholarPubMed
Ingalls, ST, Xu, Y & Hoppel, CL (1995) Determination of plasma non-esterified fatty acids and triglyceride fatty acids by gas chromatography of their methyl esters after isolation by column chromatography on silica gel [see comments]. Journal of Chromatography B 666, 1–12.CrossRefGoogle ScholarPubMed
Jensen, MD, Caruso, M, Heiling, V & Miles, JM (1989) Insulin regulation of lipolysis in nondiabetic and IDDM subjects. Diabetes 38, 1595–1601.CrossRefGoogle ScholarPubMed
Jensen, MD, Haymond, MW, Gerich, JE, Cryer, PE & Miles, JM (1987) Lipolysis during fasting Decreased suppression by insulin and increased stimulation by epinephrine. Journal of Clinical Investigation 79, 207–213.CrossRefGoogle ScholarPubMed
Klein, S, Holland, OB & Wolfe, RR (1990) Importance of blood glucose concentration in regulating lipolysis during fasting in humans. American Journal of Physiology 258, E32–E39.Google ScholarPubMed
Klein, S, Peters, EJ, Holland, OB & Wolfe, RR (1989) Effect of short- and long-term beta-adrenergic blockade on lipolysis during fasting in humans. American Journal of Physiology 257, E65–E73.Google Scholar
Klein, S & Wolfe, RR (1992) Carbohydrate restriction regulates the adaptive response to fasting. American Journal of Physiology 262, E631–E636.Google ScholarPubMed
Klein, S, Young, VR, Blackburn, GL, Bistrian, BR & Wolfe, RR (1986) Palmitate and glycerol kinetics during brief starvation in normal weight young adult and elderly subjects. Journal of Clinical Investigation 78, 928–933.CrossRefGoogle ScholarPubMed
Nurjhan, N, Consoli, A & Gerich, J (1992) Increased lipolysis and its consequences on gluconeogenesis in non-insulin-dependent diabetes mellitus. Journal of Clinical Investigation 89, 169–175.CrossRefGoogle ScholarPubMed
Patterson, BW, Zhao, G & Klein, S (1998) Improved accuracy and precision of gas chromatography/mass spectrometry measurements for metabolic tracers. Metabolism: Clinical and Experimental 47, 706–712.CrossRefGoogle ScholarPubMed
Puhakainen, I, Koivisto, VA & Yki-Jarvinen, H (1992) Lipolysis and gluconeogenesis from glycerol are increased in patients with noninsulin-dependent diabetes mellitus. Journal of Clinical Endocrinology and Metabolism 75, 789–794.Google ScholarPubMed
Rosenblatt, J & Wolfe, RR (1998) Calculation of substrate flux using stable isotopes. American Journal of Physiology 254, E526–E531.Google Scholar
Roust, LR, Hammel, KD & Jensen, MD (1994) Effects of iso-energetic, low-fat diets on energy metabolism in lean and obese women. American Journal of Clinical Nutrition 60, 470–475.CrossRefGoogle Scholar
Whitley, HA, Humphreys, SM, Samra, JS, Campbell, IT, MacLaren, DP, Reilly, T & Frayn, KN (1997) Metabolic responses to iso-energetic meals containing different proportions of carbohydrate and fat. British Journal of Nutrition 78, 15–26.CrossRefGoogle Scholar
Wolfe, RR, Peters, EJ, Klein, S, Holland, OB, Rosenblatt, J & Gary, H Jr (1987) Effect of short-term fasting on lipolytic responsiveness in normal and obese human subjects. American Journal of Physiology 252, E189–E196.Google ScholarPubMed
Wolfe, RR, Shaw, JH & Durkot, MJ (1985) Effect of sepsis on VLDL kinetics: responses in basal state and during glucose infusion. American Journal of Physiology 248, E732–E740.Google ScholarPubMed