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The effect of a nutritionally-balanced cassava (Manihot esculenta Crantz) diet on endocrine function using the dog as a model 1. Pancreas

Published online by Cambridge University Press:  09 March 2007

Beryl P. Kamalu
Beryl P. Kamalu
Affiliation:
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Abstract

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Growing dogs were divided into three groups and were fed on a control (rice) diet, a diet in which cassava (Manihot esculenta Crantz; gari) was used as the carbohydrate source, and the rice diet to which cyanide (equivalent to that present in gari) was added. Each group consumed its diet for 14 weeks, during which plasma thiocyanate concentration and plasma lipase (EC 3.1.1.3) activity were monitored. Plasma free amino acids were determined from pooled samples taken at the end of the experimental period, and the insulin status of the dogs was evaluated using the gluconeogenic index. The dogs were killed and the pancreas examined for histopathology. Dogs fed on both gari diet and the rice+cyanide diet generated significant amounts of thiocyanate when compared with the controls, with the rice+cyanide group having higher plasma thiocyanate than the gari group (P < 0.01). Plasma lipase activity rose significantly at the end of the experimental period in the dogs fed on gari (P < 0.05). Gluconeogenesis from protein was greatest in the dogs fed on gari, five times greater than that in the control dogs, while gluconeogenesis from protein in the dogs fed on rice+cyanide was approximately twice as high as that of the control dogs. Histopathological examination of the pancreas showed haemorrhage, necrosis, fibrosis and atrophy of the acinar tissue and fibrosis of the islets of Langerhans in the dogs fed on gari. The pancreas of the dogs fed on rice+cyanide showed similar lesions but haemorrhage was not prominent and fibrosis was more marked. The present study indicated that a hypoinsulinaemia developed which was more severe in the animals fed on gari than in the dogs fed on the rice+cyanide diet and that the condition was not related to the level of plasma thiocyanate or the histopathology observed.

Keywords

Cassava dietDog
Type
Nutritional Effects on Endocrine Function
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 365 - 372
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Abu-Bakare, A., Gill, G. V., Taylor, R. & Alberti, K. G. M. M. (1986). Tropical or malnutrition-related diabetes: A real syndrome? Lancet, i, 1135–1138.CrossRefGoogle Scholar
Adams, C. W. M., Fernand, V. S. V. & Schrieden, H. (1958). Histochemistry of a condition resembling kwashiorkor produced in rodents by a low protein-high carbohydrate diet (cassava). British Journal of Experimental Pathology 39, 393–404.Google ScholarPubMed
Barej, W., Ostaszewski, P. & Szcsygiel, M. (1981). Could the plasma amino acid concentrations indicate the body protein turnover in sheep? VIth International Symposium on Amino Acid Metabolism,Serock, Poland.Google Scholar
Barrett, M. D. F., Hill, D. C., Alexander, J. C. & Zitnak, A. (1977) Fate of orally-dosed linamarin in the rat. Canadian Journal of Physiology and Pharmacology 55, 134–136.CrossRefGoogle ScholarPubMed
Bowler, R. G. (1944). The determination of thiocyanate in blood serum. Biochemical Journal 38, 385–386.CrossRefGoogle ScholarPubMed
Chilaka, F. C. (1984). An investigation of the effects of high and prolonged cassava diets on some drug-metabolising enzymes of rat liver. M.Sc. Thesis, University of Nigeria, Nsukka.Google Scholar
Conn, E. E. (1969). Cyanogenic glycosides. Agricultural and Food Chemistry 17, 519–526.CrossRefGoogle Scholar
Cosby, E. L. & Summer, J. B. (1945). Rhodanese. Archives of Biochemistry 7, 457–460.Google Scholar
Felig, P., Wahoen, J., Sherwin, R. & Palaiologos, G. (1977). Amino acid and protein metabolism in diabetes mellitus. Archives of International Medicine 137, 507–513.CrossRefGoogle ScholarPubMed
Gaines Dog Research Center (1968). Basic Guide to Canine Nutrition, 2nd ed. White Plains, New York: General Foods Corporation.Google Scholar
Geevarghese, P. J. (1982). Cassava diet, tropical calcifying pancreatitis and pancreatic diabetes. In Cassava Toxicity and Thyroid: Research and Public Health Issues. Proceedings of a Workshop, Ottawa. [Delange, F. and Ahluwalia, R., editors]. International Development Research Centre Monograph 207e pp. 77–78, Ottawa: International Development Research Centre.Google Scholar
Maduagwu, E. N. & Umoh, I. B. (1986). Biliary excretion of linamarin in the Wistar rat after a single dose. Biochemical Pharmacology 35, 3003–3006.CrossRefGoogle ScholarPubMed
Massey, V. & Alberty, R. A. (1954). Ionisation constants of fumarase. Biochimica et Biophysica Acta 13, 354–359.CrossRefGoogle ScholarPubMed
Munro, H. N. & Allison, J. B. (1964). Mammalian Protein Metabolism. Vol. 1. New York: Academic Press.Google Scholar
Nartey, F. (1968). Studies on cassava, Manihot utilissima Pohl, I. Cyanogenesis: The biochemistry of linamarin and lotaustrolin in etiolated seedlings. Phytochemistry 7, 1307–1312.CrossRefGoogle Scholar
Philbrick, D. J., Hill, D. C. & Alexander, J. C. (1977). Physiological and biochemical changes associated with linamarin administration to rats. Toxicology and Pharmacology 42, 539–551.CrossRefGoogle ScholarPubMed
Prior, R. L. & Smith, S. B. (1982). Hormonal effects on partitioning of nutrients for tissue growth: role of insulin. Federation Proceedings 41, 2545–2549.Google ScholarPubMed
Sarles, H. (1973). An international survey on nutrition and pancreatitis. Digestion 9, 389–403.CrossRefGoogle ScholarPubMed
Strombeck, D. R., Wheeldon, E. & Harrold, D. (1984). Model of chronic pancreatitis in the dog. American Journal of Veterinary Research 45, 131–136.Google ScholarPubMed
Turner, C. D. & Bagnara, J. T. (1971). General Endocrinology, 5th ed. Philadelphia: W. B. Saunders Co.Google Scholar
Winkler, W. O. (1958). Report on methods for glucosidal HCN in lima beans. Journal of the Association of Agricultural Chemists 41, 282–287.Google Scholar