Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-30T15:10:25.103Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of a dietary supplement of n-3 polyunsaturated fat on platelet lipid composition, platelet function and platelet plasma membrane fluidity in healthy volunteers

Published online by Cambridge University Press:  09 March 2007

M. J. Gibney  and
Caroline Bolton-Smith
M. J. Gibney
Affiliation:
Department of Nutrition, University of Southampton, Southampton SO9 5NH
Caroline Bolton-Smith
Affiliation:
Department of Nutrition, University of Southampton, Southampton SO9 5NH
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Eight healthy male volunteers (aged 22–39 years) supplemented their normal daily diet with 15 g encapsulated fish oil (MaxEPA) for a 6 week period. Fasting blood samples were taken before, at the completion of and 3 months after the period of supplementation.

2. Evaluation of nutrient intakes showed that the intake of n-3 polyunsaturated fatty acids rose significantly (P < 0·01) during supplementation. This was reflected in changes in the fatty acid composition of platelet phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) without any changes in phosphatidyl serine, phosphatidyl inositol or sphingomyelin.

3. In both PC and PE there were significant (P < 0·05) increases in the levels of 18:1 n-9 and 20:5n-3 fatty acids and a significant (P < 0·05) decrease in 20:4n-6 during supplementation. 16:0 rose significantly in PC (P < 0·05) while in PE, 18:0 fell and both 22:5n-3 and 22:6n-3 rose significantly (P < 0·05).

4. There were no significant effects of fish-oil supplementation on serum lipids, platelet cholesterol: phospholipid, collagen-induced platelet aggregation or collagen-induced platelet thromboxane B2 production. However, there was a significant correlation (P < 0·001; r+0·63) between total phospholipid arachidonic acid and platelet thromboxane production.

5. The fluorescent probe 1,6-diphenyl-1,3,5-hexatriene was used to determine whether fish-oil supplementation altered fluorescence polarization of isolated platelet plasma membrane and, by inference, platelet plasma membrane fluidity. No significant effect of fish-oil supplementation on fluorescence polarization was seen.

Type
Clinical and Human Nutrition Papers: Studies in Man
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 5 - 12
Copyright
Copyright © The Nutrition Society 1988

References

Ahmed, A. A. & Holub, B. (1984). Journal of Lipids 19, 617–724.CrossRefGoogle Scholar
Arnich, I. & Wallach, D. F. H. (1971). Biochimica et Biophysica Acta 233, 334–347.Google Scholar
Barber, A. J., Peppers, D. S. & Jamieson, G. M. (1971). Thrombosis, Diathetica et Haemorrhagica 25, 38–57.Google Scholar
Berlin, E., Shapiro, S. G. & Kilman, P. G. (1987). Atherosclerosis 63, 85–96.CrossRefGoogle Scholar
Brox, J. H., Killie, J. E., Gunnes, S. & Norboy, A. (1981). Thrombosis and Haemostasis 46, 604–611.Google Scholar
Church, J. P., Judd, J. T., Young, C. W., Kelsay, J. L. & Kim, W. W. (1984). American Journal of Clinical Nutrition 40, 1338–1344.CrossRefGoogle Scholar
Clandinin, M. T., Field, C. J., Hargreaves, L. M. & Fsigmond, E. (1985). Canadian Journal of Physiology and Pharmacology 63, 546–556.CrossRefGoogle Scholar
Fisher, S. & Weber, P. C. (1983). Biochemical and Biophysical Research Communications 116, 1091–1099.CrossRefGoogle Scholar
Galloway, J. H., Cartwright, I. J., Woodcock, B. E., Greaves, M., Russel, G. G. & Preston, F. E.(1985). Clinical Science 68, 449–454.CrossRefGoogle Scholar
Gamble, W., Vaughan, M., Kruth, H. S. & Avigan, J. (1978). Journal of Lipid Research 19, 1068–1078.CrossRefGoogle Scholar
Goodnight, S. H., Harris, W. S. & Connor, W. E. (1981). Blood 58, 880–885.CrossRefGoogle Scholar
Harris, W. S., Connor, W. E., Inkeles, S. B. & Illingworth, D. R. (1984). Metabolism 33, 1016–1019.CrossRefGoogle Scholar
Herold, P. M. & Kinsella, J. E. (1986). American Journal of Clinical Nutrition 43, 566–598.CrossRefGoogle Scholar
Hirai, A., Terano, T., Hamazaki, T., Sajiki, J., Saito, H., Tahara, K., Tamura, Y. & Kumagai, A. (1983). Thrombosis Research 31, 29–40.CrossRefGoogle Scholar
Houslay, M. D., Metcalfe, J. C., Warren, G. B., Hesketh, T. R. & Smith, G. A. (1976). Biochimica et Biophysica Acta 436, 495–504.CrossRefGoogle Scholar
Illingworth, D. R., Harris, W. S. & Connor, W. E. (1984). Atherosclerosis 4, 270–275.Google Scholar
Kannagi, R., Koizumi, K. & Masuda, T. (1981). Journal of Biological Chemistry, 256, 1177–1184.CrossRefGoogle Scholar
Paul, A. A. & Southgate, D. A. T. (1978). McCance and Widdowson's The Composition of Foods, 4th ed. London: H.M. Stationery Office.Google Scholar
Phillipson, B. E., Rothrock, D. W., Connor, W. E., Harris, W. S. & Illingworth, D. R. (1985). New England Journal of Medicine 312, 1210–1216.CrossRefGoogle Scholar
Raheja, R. K., Vaur, C., Singh, A. & Bhatia, I. S. (1973). Journal of Lipid Research 14, 695–697.CrossRefGoogle Scholar
Rand, M. C., Hennisen, A. A. M. & Hornstra, G. (1986). Atherosclerosis 62, 267–276.CrossRefGoogle Scholar
Reeves, V. B., Matusik, E. J. & Kelsay, J. L. (1984). American Journal of Clinical Nutrition 40, 1345–1351.CrossRefGoogle Scholar
Ross, R. (1986). New England Journal of Medicine 314, 488–500.CrossRefGoogle Scholar
Salmon, J. A. & Terano, T. (1985). Proceedings of the Nutrition Society 44, 385–389.CrossRefGoogle Scholar
Sanders, T. A. B. (1985). Proceedings of the Nutrition Society 44, 391–397.CrossRefGoogle Scholar
Sanders, T. A. B. & Hochland, M. C. (1983). British Journal of Nutrition 50, 521–529.CrossRefGoogle Scholar
Sanders, T. A. B. & Roshani, F. (1983). Clinical Science 64, 91–99.CrossRefGoogle Scholar
Sanders, T. A. B., Vickers, M. & Haines, A. P. (1981). Clinical Science 61, 317–324.CrossRefGoogle Scholar
Seiss, W., Scherer, B., Bohlig, B., Roth, P., Kurzmann, I. & Weber, P. C. (1980). Lancet ii, 441–444.CrossRefGoogle Scholar
Seynor, R., Verel, D. & Gillott, T. (1984). Atherosclerosis 50, 3–10.CrossRefGoogle Scholar
Shinitzky, M. & Barenholz, Y. (1978). Biochimica et Biophysica Acta 515, 367–394.CrossRefGoogle Scholar
Stubbs, C. D. (1983). Essays in Biochemistry 19, 1–39.Google Scholar
Taylor, D. G., Williams, V. M. & Crawford, N. (1977). Biochimica et Biophysica Acta 465, 321–331.Google Scholar
Terano, T., Hirai, A., Hamazaki, T., Kobayashi, S., Fugita, T., Tamura, Y. & Kumagai, A. (1983). Atherosclerosis 46, 321–331.CrossRefGoogle Scholar
Tisdale, D. (1976). Methods in Enzymology 10, 213–215.CrossRefGoogle Scholar