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Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum

Published online by Cambridge University Press:  08 March 2007

M. Ivan ,
K.M. Koenig ,
D.P. Morgavi ,
L.M. Rode ,
C.J. Newbold  and
T. Entz
M. Ivan*
Affiliation:
Agriculture and Agri-Food CanadaPO Box 90, 2000 Route 108 East Lennoxville, Quebec, J1M 1Z3Canada
K.M. Koenig
Affiliation:
Agriculture and Agri-Food CanadaPO Box 3000, Lethbridge, Alberta, T1J 4B1Canada
D.P. Morgavi
Affiliation:
Institute National de la Recherche Agronomique (INRA)Centre de Clermont-Ferrand Theix, 63122 Saint Genè ChampanelleFrance
L.M. Rode
Affiliation:
Rosebud Technologies Development Ltd3302 Beauvais Place S., Lethbridge, Alberta, T1K 3J5Canada
C.J. Newbold
Affiliation:
University of WalesInstitute of Rural Sciences, Lianbadarn Campus, Aberystwyth, Ceredigion, SY23 3ALUK
T. Entz
Affiliation:
Agriculture and Agri-Food CanadaPO Box 3000, Lethbridge, Alberta, T1J 4B1Canada
*
*Corresponding author: fax +1 819 564 5507, email [email protected]
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Abstract

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Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. 15Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P<0·1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P<0·05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P>0·05) in these variables between the two monofauna groups. Protozoal N accounted for 8% of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P>0·05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P<0·05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.

Keywords

EntodiniumPolyplastronRumen ciliate protozoaSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 469 - 476
Copyright
Copyright © The Nutrition Society 2006

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