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Does the glycogen synthase (EC 2.4.1.21) of brown adipose tissue play a regulatory role in glucose homeostasis?

Published online by Cambridge University Press:  09 March 2007

Zecharia Madar  and
Ayalah Harel
Zecharia Madar
Affiliation:
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Ayalah Harel
Affiliation:
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
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Abstract

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Glycogen synthase (GS) activity was characterized in rat brown adipose tissue (BAT) and the activity was found to be much higher than that in white adipose tissue. Prolonged starvation had no effect on the active form of GS, as found in the liver and muscle. The GS activity was similar in BAT of rats housed in an animal room (21+± 1°) whether they were fed on high-carbohydrate, high-fat, or stock diets. Acclimatizatioin of rats to cold (4+± 1°) for 2 weeks significantly increased GS activity. This increase in the cold was fivefold greater when rats were fed on high-carbohydrate diets than in control rats at room temperature fed on an identical diet. The increase was accompanied by a large accumulation of glycogen in BAT. It was concluded that GS may play an important role in BAT and may contribute to the control of blood glucose in a cold environment. Its relevance to thermogenesis requires further elucidation.

Keywords

FastingHigh-carbohydrate dietHigh-fat dietGlycogen synthase
Type
Carbohydrate Metabolism
Information
British Journal of Nutrition , Volume 66 , Issue 1 , July 1991 , pp. 95 - 104
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Cawthorne, M. A. (1989). Does brown adipose tissue have a role to play in glucose homeostasis? Proceedings of the Nutrition Society 48, 207–214.CrossRefGoogle ScholarPubMed
Cooney, G. J. & Newsholme, E. A. (1982). The maximum capacity of glycolysis in brown adipose tissue and its relationship to control of the blood glucose concentration. FEBS Letters 148, 198–200.CrossRefGoogle ScholarPubMed
Cooney, G. J. & Newsholme, E. A. (1984). Does brown adipose tissue have a metabolic role in the rat? Trends in Biochemical Sciences 9, 303–305.CrossRefGoogle Scholar
Cooney, G. J., Taegtmeyer, H. & Newsholme, E. A. (1981). Tricarboxylic acid cycle flux and enzyme activities in the isolated working rat heart. Biochemical Journal 200, 701–703.CrossRefGoogle ScholarPubMed
Denton, R. M., McCormack, J. G. & Marshall, S. E. (1984). Persistence of the effect of insulin on pyruvate dehydrogenase activity in rat white and brown adipose tissue during the preparation and subsequent incubation of mitochondria. Biochemical Journal 221, 441–452.CrossRefGoogle Scholar
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.CrossRefGoogle Scholar
Ferre, P., Burnol, A. F., Leturque, A., Terretaz, J., Penicaud, L., Jeanrenaud, B. & Girard, J. (1986). Glucose utilization in vivo and insulin-sensitivity in brown adipose tissue in various physiological and pathological conditions. Biochemical Journal 233, 249–252.CrossRefGoogle ScholarPubMed
Gibbins, J. M. & Denton, R. M. (1986). Glycogen metabolism in rat interscapular brown adipose tissue. Biochemical Society Transactions 14, 278–279.CrossRefGoogle Scholar
Greco-Perotto, R., Assimacopoulos-Jeannet, F. & Jeanrenaud, B. (1987). Insulin modifies the properties in rat brown adipose tissue. Biochemical Journal 247, 63–68.CrossRefGoogle ScholarPubMed
Hellertstein, M. K., Greenblatt, D. J. & Munro, H. (1986). Glycoconjugates as noninvasive probes of intrahepatic metabolism: Pathways of glucose entry into compartmentalized hepatic UDP-glucose pools during glycogen accumulation. Proceedings of the National Academy of Sciences U.S.A. 83, 7044–7048.CrossRefGoogle Scholar
Himns-Hagen, J. (1985). Brown adipose tissue metabolism and thermogenesis. Annual Review of Nutrition 5, 69–94.CrossRefGoogle Scholar
Howland, R. J. & Bond, K. D. (1987). Cold-acclimation increases insulin sensitivity of brown adipocytes. Hormone and Metabolic Research 19, 503–504.CrossRefGoogle ScholarPubMed
Isler, D., Hill, H. P. & Meier, M. K. (1987). Glucose metabolism in isolated brown adipocytes under β-adrenergic stimulation. Quantitative contribution of glucose to total thermogenesis. Biochemical Journal 245, 789–793.CrossRefGoogle ScholarPubMed
Kaslow, H. R. & Eishner, H. D. (1984). Fasting and diabetes alter adipose tissue glycogen synthase. American Journal of Physiology 247, E581–E585.Google ScholarPubMed
Kochan, R. G., Lamb, D. R., Lutz, S. A., Perrill, C. V., Reimann, E. M. & Schlender, K. K. (1979). Glycogen synthase activation in human skeletal muscle: effects of diet and exercise. American Journal of Physiology 236, E660–E666.Google ScholarPubMed
McCormack, J. G. (1982). The regulation of fatty acid synthesis in brown adipose tissue by insulin. Progress in Lipids Research 21, 195–223.CrossRefGoogle ScholarPubMed
McCormack, J. G., Gibbins, J. M. & Denton, R. M. (1986). Lipogenesis in brown adipose tissue and its regulation. Biochemical Society Transactions 14, 227–229.CrossRefGoogle ScholarPubMed
Madar, Z. (1989). Pyruvate dehydrogenase and glycogen synthase activity at transition from fasted and fed state. Biochemical Medicine and Metabolic Biology 41, 93–104.CrossRefGoogle Scholar
Madar, Z., Bell, J. M. & Mandarino, L. J. (1987). Glycogen synthase kinetics in isolated human adipocytes: an in vitro model for the effect of insulin on glycogen synthase. Biochemical Medicine and Metabolic Biology 38, 265–271.CrossRefGoogle Scholar
Mandarino, L. J., Madar, Z., Kolterman, O. G., Bell, H. M. & Olefsky, J. M. (1986). Adipocytes, glucogen synthase and pyruvate dehydrogenase in obese and type II diabetic subjects. American Journal of Physiology 251, E489–E496.Google Scholar
Nedergaard, J. & Lindberg, O. (1982). The brown fat-cell, review. International Review of Cytology 74, 187–286.CrossRefGoogle Scholar
Serra, F., Bonet, L. & Palou, A. (1987). Amino-acid enzyme activities in brown and white adipose tissues and in the liver of cafeteria rats. Effects of 24 hours starving. Archives Internationales de Physiologie et de Biochimie 95, 263–268.Google Scholar
Sugden, M. C., Watts, D. I., Marshall, C. E. & McCormack, J. G. (1982). Brown-adipose-tissue lipogenesis in starvation: effects of insulin and (-) hydroxycitrate. Bioscience Reports 2, 289–297.CrossRefGoogle ScholarPubMed
Trayhurn, P. (1979). Fatty acid synthesis in vivo in brown adipose tissue, liver and white adipose tissue of the cold-acclimated rat. FEBS Letters 104, 13–16.CrossRefGoogle ScholarPubMed
Wearire, P. J. & Kanagasabai, T. F. (1982). Effect of high-carbohydrate diets on lipogenesis in rat interscapular brown adipose tissue. Biochemical Journal 206, 667–669.CrossRefGoogle Scholar
Young, P., Cawthorne, M. A., Levy, A. L. & Wilson, K. (1984). Reduced maximum capacity of glycolysis in brown adipose tissue of genetically obese, diabetic (db/db) mice and its restoration following treatment with a thermogenic β-adrenoceptor agonist. FEBS Letters 176, 16–20.CrossRefGoogle ScholarPubMed