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The distribution of peptidase activity in the small intestine of sheep

Published online by Cambridge University Press:  09 March 2007

R. I. Richardson  and
A. R. P. Jouan
R. I. Richardson
Affiliation:
Department of Agricultural Biochemistry and Nutrition, Faculty of Agriculture, The University, Newcastle upon Tyne NE1 7RU
A. R. P. Jouan
Affiliation:
Department of Agricultural Biochemistry and Nutrition, Faculty of Agriculture, The University, Newcastle upon Tyne NE1 7RU
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Abstract

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1. The activity of peptidase enzyme(s) was measured in homogenates of mucosa taken at slaughter from sites along the length of sheep small intestine.

2. The activity of the homogenate towards six dipeptides showed a similar pattern of distribution for each substrate. Activity was lowest in the proximal duodenum, rising through the jejunum to peak in the mid-ileum and declining towards the terminal ileum. The distribution of activity towards two tripeptides was similar.

3. Studies using the inhibitor p-hydroxymercuribenzoate showed that the activity towards the dipeptides was mainly localized in the cytosol fraction, while that towards the tripeptides was evenly distributed between the cytosol and the brush-border and activity towards a tetrapeptide was mainly associated with the brush-border.

4. The results are discussed in relation to the site and mechanism of protein digestion and absorption in the ruminant small intestine.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 55 , Issue 1 , January 1986 , pp. 149 - 156
Copyright
Copyright © The Nutrition Society 1986

References

REFERENCES

Adibi, S. A. (1971). Journal of Clinical Investigation 50, 2266–2275.CrossRefGoogle Scholar
Baumrucker, C. R. & Davis, C. L. (1980). Journal of Dairy Science 63, 379–384.CrossRefGoogle Scholar
Ben-Ghedalia, D., Tagari, H., Bondi, A. & Tadmor, A. (1974). British Journal of Nutrition 31, 125–142.CrossRefGoogle Scholar
Das, M. & Radhakrishnan, A. N. (1973). Biochemical Journal 135, 609–615.CrossRefGoogle Scholar
Das, M. & Radhakrishnan, A. N. (1974). Clinical Science and Molecular Medicine 46, 501–510.Google Scholar
Dixon, M. & Webb, E. C. (1964). Enzymes, 2nd ed. London: Longmans.Google Scholar
Dolly, J. O., Dillon, A., Duffy, M. J. & Fottrell, P. F. (1971). Clinica Chimica Acta 31, 55–62.CrossRefGoogle Scholar
Duomeng, C. & Maroux, S. (1979). Biochemical Journal 177, 801–808.CrossRefGoogle Scholar
Fujita, M., Parsons, D. S. & Wojnarowska, F. (1972). Journal of Physiology 227, 377–394.CrossRefGoogle Scholar
Heizer, W. D., Kerley, R. L. & Isselbacher, K. J. (1972). Biochimica et Biophysica Acta 264, 450–461.CrossRefGoogle Scholar
Johns, J. T. & Bergen, W. G. (1973). Journal of Nutrition 103, 1581–1586.CrossRefGoogle Scholar
Josefsson, L. & Lindberg, T. (1965). Biochimica et Biophysica Acta 105, 162–166.CrossRefGoogle Scholar
Kim, Y. S., Birtwhistle, W. & Kim, Y. W. (1972). Journal of Clinical Investigation 51, 1419–1430.CrossRefGoogle Scholar
McAllan, A. B. (1981). British Journal of Nutrition 46, 431–440.CrossRefGoogle Scholar
McCarthy, D. M. & Kim, Y. S. (1973). Journal of Clinical Investigation 52, 942–951.CrossRefGoogle Scholar
Mathews, D. M. (1975). Physiological Reviews 55, 537–608.CrossRefGoogle Scholar
Miller, G. L. (1959). Analytical Chemistry 31, 964.CrossRefGoogle Scholar
Nicholson, J. A. & Kim, Y. S. (1975). Analytical Biochemistry 63, 110–117.CrossRefGoogle Scholar
Nicholson, J. A. & Peters, T. J. (1979). European Journal of Clinical Investigation 9, 349–354.CrossRefGoogle Scholar
Nixon, S. E. & Mawer, G. E. (1970 a). British Journal of Nutrition 24, 227–240.CrossRefGoogle Scholar
Nixon, S. E. & Mawer, G. E. (1970 b). British Journal of Nutrition 24, 241–258.CrossRefGoogle Scholar
Peters, T. J. (1970). Biochemical Journal 120, 195–203.CrossRefGoogle Scholar
Philips, W. A., Webb, K. E. Jr & Fontenot, J. P. (1976). Journal of Animal Science 42, 201–207.CrossRefGoogle Scholar
Robinson, G. B. & Shaw, B. (1960). Biochemical Journal 77, 351–356.CrossRefGoogle Scholar
Silk, D. B. A., Mars, T. C., Addison, J. M., Burston, D., Clark, M. L. & Mathews, D. M. (1973). Clinical Science and Molecular Medicine 45, 715–719.Google Scholar
Sterchi, E. E. & Woodley, J. F. (1980 a). Clinica Chimica Acta 102, 49–56.CrossRefGoogle Scholar
Sterchi, E. E. & Woodley, J. F. (1980 b). Clinica Chimica Acta 102, 57–65.CrossRefGoogle Scholar
Symons, L. E. A. & Jones, W. O. (1966). Comparative Biochemistry and Physiology 18, 71–82.CrossRefGoogle Scholar
Tagari, H. & Bergman, E. N. (1978). Journal of Nutrition 108, 790–803.CrossRefGoogle Scholar
Wahle, K. W. J., Weekes, T. E. C. & Sherratt, H. S. A. (1972). Comparative Biochemistry and Physiology 41B, 759–769.Google Scholar
Williams, V. J. (1969). Comparative Biochemistry and Physiology 29, 865–870.CrossRefGoogle Scholar