Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-25T02:02:47.470Z Has data issue: false hasContentIssue false
  • English
  • Français

Digestibilities of nitrogen compounds in rumen bacteria and in other components of digesta in the small intestine of the young steer

Published online by Cambridge University Press:  09 March 2007

D. N. Salter  and
R. H. Smith
D. N. Salter
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
R. H. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Mixed bacteria were obtained from the rumen contents of steers and were taken at different times after the animals had been given different feeds. The feeds contained [15N]urea and in some experiments Na235SO4 so that the bacteria were labelled with these isotopes.

2. Samples of labelled bacteria were subjected to a simulated abomasal digestion with pepsin and the digests were infused with a non-absorbed marker, polyethylene glycol, into the duodenums of test steers equipped with re-entrant ileal cannulas. Except for samples taken after a 24 h fast, which sometimes gave somewhat lower values, mean values for digestibilities of 15N and 35S in the small intestine were 0.79 and 0.85 respectively.

3. The corresponding value for the digestibility of 15N in similarly-treated wheat-leaf protein concentrate was 0.86.

4. Net digestibilities of total N in the small intestine of the test steers when they were given either a mainly protein-free diet of straw, tapioca and urea or a diet of flaked maize and hay were approximately 0.62.

5. From these and other values it was calculated that approximately 5.2g intestinally-secreted endogenous N/d flowed through the ileum of a 100 kg steer.

Type
Research Article
Information
British Journal of Nutrition , Volume 38 , Issue 2 , September 1977 , pp. 207 - 216
Copyright
Copyright © The Nutrition Society 1977

References

Ben-Ghedalia, D., Tagari, H. & Bondi, A. (1974). Br. J. Nutr. 31, 125.CrossRefGoogle Scholar
Bergen, W. G., Purser, D. B. & Cline, J. H. (1967). J. Nutr. 92, 357.CrossRefGoogle Scholar
Bergen, W. G., Purser, D. B. & Cline, J. H. (1968). J. Dairy Sci. 51, 1698.CrossRefGoogle Scholar
Bird, P. R. (1972). Aust J. biol. Sci. 25, 195.CrossRefGoogle Scholar
Clarke, E. M. W., Ellinger, G. M. & Phillipson, A. T. (1966). Proc. R. Soc. B 166, 63.Google Scholar
Coelho, da, Silva, J. F., Seeley, R. C., Beever, D. E., Prescott, J. D. H. & Armstrong, D. G. (1972). Br. J. Nutr. 28, 357.CrossRefGoogle Scholar
Daneshvar, K. (1976). Studies with 15N of the incorporation in the rumen of non-protein nitrogen into microbial protein and nucleic acids. PhD thesis, University of Reading.Google Scholar
Daneshvar, K., Salter, D. N. & Smith, R. H. (1976). Proc. Nutr. Soc. 35, 54A.Google Scholar
Egan, A. R. (1974). Aust. J. agric. Res. 25, 613.CrossRefGoogle Scholar
Ferrari, A. (1960). Ann N.Y. Acad. Sci. 87, 792.CrossRefGoogle Scholar
Harrop, C. J. F. (1974). J. agric. Sci., Camb. 83, 249.CrossRefGoogle Scholar
Hogan, J. P. (1973). Aust. J. agric. Res. 24, 587.CrossRefGoogle Scholar
Hogan, J. O. & Weston, R. H. (1968). Proc. Aust. Soc. Anim. Prod. 7, 364.Google Scholar
Horn, J. (1975). Factors affecting the absorption of magnesium in the stomachs and small intestine of the calf. PhD thesis, University of Reading.Google Scholar
Lloyd-Jones, C. P., Salter, D. N., & Adam, J. (1975). Analyst, Lond. 100, 891.CrossRefGoogle Scholar
McNaught, M. L., Owen, E. C., Henry, K. M. & Kon, S. K. (1954). Biochem. J. 56, 151.CrossRefGoogle Scholar
Mason, V. C. & Milne, G. (1971). J. agric. Sci., Camb. 77, 99.CrossRefGoogle Scholar
Morrison, J. E. & Pirie, N. W. (1961). J. Sci. Fd Agric. 12, 1.CrossRefGoogle Scholar
Salter, D. N. & Smith, R. H. (1974). Proc. Nutr. Soc. 33, 42AGoogle Scholar
Salton, M. R. J. (1960). In The Bacteria, Vol. 1, p. 120 [Gunsalus, I. C. and Stanier, R. Y., editors]. New York and London: Academic Press.Google Scholar
Smith, R. H. (1962). Biochem J. 83, 151.CrossRefGoogle Scholar
Smith, R. H. (1964). J. Physiol., Lond. 172, 305.CrossRefGoogle Scholar
Smith, R. H. (1976). In Digestion and Metabolism in the Ruminant, p. 399 [McDonald, I. W. & Warner, A. C. I., editors]. Armidale: University of New England.Google Scholar
Smith, R. H. & McAllan, A. B. (1970). Br. J. Nutr. 24, 545.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1971). Br. J. Nutr. 25, 181.CrossRefGoogle Scholar
Van't Klooster, A. Th. & Boekholt, H. A. (1972). Neth. J. agric. Sci. 20, 272.Google Scholar