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Differential effects of n-3 and n-6 polyunsaturated fatty acids on BRCA1 and BRCA2 gene expression in breast cell lines

Published online by Cambridge University Press:  09 March 2007

Dominique J. Bernard-Gallon ,
Cécile Vissac-Sabatier ,
David Antoine-Vincent ,
Pascale G. Rio ,
Jean-Claude Maurizis ,
Pierre Fustier  and
Yves-Jean Bignon
Dominique J. Bernard-Gallon
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Cécile Vissac-Sabatier
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
David Antoine-Vincent
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Pascale G. Rio
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France INSERM U 484, 63005 Clermont-Ferrand, France
Jean-Claude Maurizis
Affiliation:
INSERM U 484, 63005 Clermont-Ferrand, France
Pierre Fustier
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Yves-Jean Bignon*
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
*
*Corresponding author: Dr Yves-Jean Bignon, fax +33 4 73 27 80 42, email [email protected]
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Abstract

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Current evidence strongly supports a role for the breast tumour suppressor genes, BRCA1 and BRCA2, in both normal development and carcinogenesis. In vitro observations reported that BRCA1 and BRCA2 are expressed in a cell cycle-dependent manner. Interestingly, differences in the actions of n-3 and n-6 polyunsaturated fatty acids have been observed: while the n-3 polyunsaturated fatty acids have been described to reduce pathological cell growth, the n-6 polyunsaturated fatty acids have been found to induce tumour proliferation. Here, we examined the expression of BRCA1 and BRCA2 in breast cell lines after treatment with polyunsaturated fatty acids. Real-time quantitative polymerase chain reaction determinations conclusively demonstrated increases in BRCA1 and BRCA2 mRNA expressions in MCF7 and MDA-MB 231 tumour cell lines after treatment with n-3 polyunsaturated fatty acids (eicosapentaenoic acid and docosahexaenoic acid), but no variation was noticed with the n-6 polyunsaturated fatty acid (arachidonic acid). On the other hand, no variation of the expression of BRCA1 and BRCA2 mRNA was detected in MCF10a normal breast cell line treated by polyunsaturated fatty acids. The level of BRCA1 and BRCA2 proteins quantified by affinity chromatography remained unchanged in tumour (MCF7, MDA-MB 231) and normal (MCF10a) breast cell lines. We suggest the presence of a possible transcriptional or post-transcriptional regulation of BRCA1 and BRCA2 after n-3 polyunsaturated fatty acid treatment in breast tumour cells.

Keywords

BRCA1BRCA2Polyunsaturated fatty acidsMammary cell linesReal-time quantitative polymerase chain reaction
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 4 , April 2002 , pp. 281 - 289
Copyright
Copyright © The Nutrition Society 2002

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