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Dietary-induced increases of disaccharidase activities in rat jejunum

Published online by Cambridge University Press:  09 March 2007

Betty K. Samulitis-Dos Santos ,
Toshinao Goda  and
Otakar Koldovsky
Betty K. Samulitis-Dos Santos
Affiliation:
Department of Pediatrics and Physiology, University of Arizona College of Medicine, Tucson, Arizona 85724, USA and The Committee on Nutritional Sciences, University of Arizona, Tucson, Arizona 85721, USA
Toshinao Goda
Affiliation:
Department of Pediatrics and Physiology, University of Arizona College of Medicine, Tucson, Arizona 85724, USA and The Committee on Nutritional Sciences, University of Arizona, Tucson, Arizona 85721, USA
Otakar Koldovsky
Affiliation:
Department of Pediatrics and Physiology, University of Arizona College of Medicine, Tucson, Arizona 85724, USA and The Committee on Nutritional Sciences, University of Arizona, Tucson, Arizona 85721, USA
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Abstract

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A study was carried out to examine whether the responsiveness of small intestinal epithelial cells to dietary carbohydrate varied during the daily 24 h cycle. The effect of sucrose on disaccharidase activities was compared during a period of decreasing disaccharidase activities, i.e. between 22.00 and 10.00 hours, and increasing disaccharidase activities, i.e. between 10.00 and 22.00 hours, in the jejunum of 7-week-old-rats. Rats were fed on a low-starch, high-fat diet (Lst; starch 5 and fat 73% of gross energy), or a high-starch, low-fat diet (Hst; starch 70 and fat 7% of gross energy). Both dietary groups exhibited typical diurnal variations in jejunal sucrase (EC 3.2.1.48), maltase (EC 3.2.1.20) and lactase (EC 3.2.1.23) activities, exhibiting a peak around 22.00 hours and a trough at approximately 10.00 hours. When rats were fed on diet Lst for 7 d and then force-fed on an isoenergetic sucrose diet (S; sucrose 40 and fat 37% of gross energy) for 6 or 12 h they exhibited increased sucrase, maltase and lactase activities compared with rats fed on diet Lst. The absolute increase in disaccharidase activities was similar regardless of the time diet S was given or whether rats were killed at 10.00 hours or at 22.00 hours. Analyses of sucrase and lactase activities along the villus–crypt columns showed that the distribution of cell cohorts that responded to diet S was not influenced by the time of introduction of diet S. These findings suggest that small intestinal epithelial cells possess the ability to respond to dietary carbohydrate throughout the daily 24 h cycle.

Keywords

Dietary carbohydrateDiurnal rhythmDisaccharidase activity
Type
Diurnal Effects in Metabolism
Information
British Journal of Nutrition , Volume 67 , Issue 2 , March 1992 , pp. 267 - 278
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

Alpers, D. H. & Tedesco, F. J. (1975) The possible role of pancreatic proteases in the turnover of intestinal brush border proteins. Biochimica et Biophysica Acta 401, 28–40.CrossRefGoogle ScholarPubMed
American Institute of Nutrition (1977) Report of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Cézard, J. P., Broyart, J. P., Cuisinier-Gleizes, P. & Mathieu, H. (1983) Sucrase-isomaltase regulation by dietary sucrose in the rat. Gastroenterology 84, 18–25.CrossRefGoogle ScholarPubMed
Dahlqvist, A. (1964) Method for assay of intestinal disaccharidases. Analytical Biochemistry 7, 18–25.CrossRefGoogle ScholarPubMed
Erlanger, B. F., Kokowsky, N. & Cohen, W. (1961) The preparation and properties of two new chromogenic substrates of trypsin. Archives of Biochemistry and Biophysics 95, 271–278.CrossRefGoogle ScholarPubMed
Furuya, S., Sitren, H. S., Zeigen, S., Offord, C. E. & Stevenson, N. R. (1979) Alterations in the circadian rhythmicity of rat small intestinal functions. Journal of Nutrition 109, 1962–1973.CrossRefGoogle ScholarPubMed
George, D. E., Lebenthal, E., Landis, M. & Lee, P. C. (1985) Circadian rhythm of the pancreatic enzymes in rat: its relation to small intestinal disaccharidases. Nutrition Research 5 651, 662.Google Scholar
Girard-Globa, A., Bourdel, G. & Lardeux, B. (1980) Regulation of protein synthesis and enzyme accumulation in the rat pancreas by amount and timing of dietary protein. Journal of Nutrition 110, 1380–1390.CrossRefGoogle ScholarPubMed
Goda, T., Bustamante, S. & Koldovsky, O. (1985) Dietary regulation of intestinal lactase and sucrase in adult rats: quantitative comparison of effect of lactose and sucrose. Journal of Pediatric Gastroenterology and Nutrition 4, 998–1008.CrossRefGoogle ScholarPubMed
Goda, T., Bustamante, S., Thornburg, W. & KoldovskY, O. (1984) Dietary-induced increase of lactase activity and in immunoreactive lactase in adult rat jejunum. Biochemical Journal 221, 261–263.CrossRefGoogle ScholarPubMed
Goda, T. & KoldovskY, O. (1985) Evidence of degradation process of sucrase-isomaltase in jejunum of adult rats. Biochemical Journal 229, 751–758.CrossRefGoogle ScholarPubMed
Goda, T. & KoldovskY, O. (1988). Dietary regulation of small intestinal disaccharidases. In World Review of Nutrition and Dietetics, Vol. 57, pp. 275–329 [Bourne, G. H., editor]. Basel, Karger: Medical Publishing Co.Google Scholar
Goda, T., Quaroni, A. & KoldovskY, O. (1988) Characterization of degradation process of sucrase-isomaltase in rat jejunum with monoclonal antibody-based enzyme-linked immunosorbent assay. Biochemical Journal 250, 41–46.CrossRefGoogle ScholarPubMed
Goda, T., Yamada, J., Bustamante, S. & KoldovskY, O. (1983) Dietary-induced rapid decrease of microvillar carbohydrase activity in at jejunoileum. American Journal of Physiology 245, G418–G423.Google ScholarPubMed
Kaufman, M. A., Korsmo, H. A. & Olsen, W. A. (1980) Circadian rhythm of intestinal sucrase activity in rats. Mechanism of enzyme change. Journal of Clinical Investigation 65, 1174–1181.CrossRefGoogle ScholarPubMed
KoldovskY, O., Asp, N. G. & Dahlqvist, A. (1969) A method for the separate assay of ‘neutral’ and ‘acid’ β-galactosidase in homogenates of rat small intestinal mucosa. Analytical Biochemistry 27, 409–418.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Riby, J. E. & Kretchmer, N. (1984) Effect of dietary sucrose on synthesis and degradation of intestinal sucrase. American Journal of Physiology 246, G757–G763.Google ScholarPubMed
Riby, J. E. & Kretchmer, N. (1985) Participation of pancreatic enzymes in the degradation of intestinal sucrase-isomaltase. Journal of Pediatric Gastroenterology and Nutrition 4, 971–979.Google ScholarPubMed
Saito, M. (1972) Daily rhythmic changes in brush border enzymes of the small intestine and kidney in rat. Biochimica et Biophysica Acta 286, 212–215.CrossRefGoogle ScholarPubMed
Saito, M., Murakami, E., Nishida, T., Fujisawa, Y. & Suda, M. (1975) Circadian rhythms in digestive enzymes in the small intestine of rats. 1. Patterns of the rhythms in various regions of the small intestine. Journal of Biochemistry 78, 475–480.CrossRefGoogle ScholarPubMed
Saito, M., Murakami, E. & Suda, M. (1976) Circadian rhythms in disaccharidases of rat small intestine and its relation to food intake. Biochimica et Biophysica Acta 421, 177–179.CrossRefGoogle ScholarPubMed
Saito, M., Sato, Y. & Suda, M. (1978) Circadian rhythm and dietary response of disaccharidase activities in isolated rat jejunum. Gastroenterology 75, 828–831.CrossRefGoogle ScholarPubMed
Semenza, G. (1986) Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and of renal tubuli. Annual Review of Cell Biology 2, 255–313.CrossRefGoogle ScholarPubMed
Shinohara, H., Tsuji, Y., Yamada, K. & Hosoya, N. (1986) Effects of carbohydrate intake on disaccharidase activity and disaccharide-evoked transmural potential difference in rat small intestine. Journal of the Japanese Society of Nutrition and Food Science 39, 35–41.CrossRefGoogle Scholar
Stevenson, N. R., Ferrigni, F., Parnicky, K., Day, S. & Fierstein, J. S. (1975) Effect of changes in feeding schedule on the diurnal rhythms and daily activity levels of intestinal brush border enzymes and transport systems. Biochimica et Biophysica Acta 406, 131–145.CrossRefGoogle ScholarPubMed
Tsuboi, K. K., Kwong, L. K., Yamada, K., Sunshine, P. & KoldovskY, O. (1985) Nature of elevated rat intestinal carbohydrase activities after high-carbohydrate diet feeding. American Journal of Physiology 249, G510–G518.Google ScholarPubMed
Yamada, K., Bustamante, S. & KoldovskY, O. (1981a) Dietary-induced rapid increase of rat jejunal sucrase and lactase activity on all regions of the villus. FEBS Letters 129, 89–92.CrossRefGoogle ScholarPubMed
Yamada, K., Bustamante, S. & KoldovskY, O. (1981b) Time- and dose-dependency of intestinal lactase activity in adult rat on starch intake. Biochimica et Biophysica Acta 676, 108–112.CrossRefGoogle ScholarPubMed