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Dietary whey protein increases liver and skeletal muscle glycogen levels in exercise-trained rats

Published online by Cambridge University Press:  08 March 2007

Masashi Morifuji ,
Kensuke Sakai ,
Chiaki Sanbongi  and
Katsumi Sugiura
Masashi Morifuji*
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Kensuke Sakai
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Chiaki Sanbongi
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Katsumi Sugiura
Affiliation:
Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
*
*Corresponding author: Dr Masashi Morifuji, fax +81 49 284 7598, email [email protected]
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Abstract

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We investigated the effect of different types of dietary protein on glycogen content in liver and skeletal muscle of exercise-trained rats. Twenty-four male Sprague-Dawley rats (approximately 100 g; n 6 per group) were divided into sedentary or exercise-trained groups with each group being fed either casein or whey protein as the source of dietary protein. Rats in the exercised groups were trained during 2 weeks using swimming exercise for 120 min/d, 6 d/week. Exercise training resulted in an increase in the skeletal muscle glycogen content. Furthermore, the whey protein group significantly increased the skeletal muscle glycogen content compared with the casein group. The increase in glycogen content in liver was significantly greater in rats fed the whey protein diet compared with those fed the casein diet. We also found that the whey protein diet increased the activity of liver glucokinase, whereas it decreased the activities of 6-phosphofructokinase and pyruvate kinase compared with the casein diet. However, hepatic total glycogen synthase activity and mRNA expression were similar with the two diets. In the skeletal muscle, whey protein decreased only 6-phosphofructokinase activity compared with casein. Total glycogen synthase activity in the skeletal muscle in the whey protein group was significantly higher than that in the casein group. The present study is the first to demonstrate that a diet based on whey protein may increase glycogen content in liver and skeletal muscle of exercise-trained rats. We also observed that whey protein regulated glycogen metabolism in these two tissues by different mechanisms.

Keywords

Whey proteinGlycogenGlycoregulatory enzymesExercise-trained rats
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 4 , April 2005 , pp. 439 - 445
Copyright
Copyright © The Nutrition Society 2005

References

Armstrong, JL, Bonavaud, SM, Toole, BJ & Yeaman, SJ (2001) Regulation of glycogen synthesis by amino acids in cultured human muscle cells. J Biol Chem 276, 952–956.Google Scholar
Bak, JF, Moller, N, Schmitz, O, Richter, EA & Pedersen, O (1991) Effects of hyperinsulinemia and hyperglycemia on insulin receptor function and glycogen synthase activation in skeletal muscle of normal man. Metabolism 40, 830–835.Google Scholar
Baquet, A, Hue, L, Meijer, AJ, van Woerkom, GM & Plomp, PJ (1990) Swelling of rat hepatocytes stimulates glycogen synthesis. J Biol Chem 265, 955–959.Google Scholar
Bogardus, C, Lillioja, S, Stone, K & Mott, D (1984) Correlation between muscle glycogen synthase activity and in vivo insulin action in man. J Clin Invest 73, 1185–1190.Google Scholar
Burcelin, R, del, Carmen, Munoz, M, Guillam, MT & Thorens, B (2000) Liver hyperplasia and paradoxical regulation of glycogen metabolism and glucose-sensitive gene expression in GLUT2-null hepatocytes. Further evidence for the existence of a membrane-based glucose release pathway. J Biol Chem 275, 10930–10936.Google Scholar
Burcelin, R, Printz, RL, Kande, J, Assan, R, Granner, DK & Girard, J (1993) Regulation of glucose transporter and hexokinase II expression in tissues of diabetic rats. Am J Physiol 265, E392–E401.Google Scholar
Cartee, GD, Young, DA, Sleeper, MD, Zierath, J, Wallberg-Henriksson, H & Holloszy, JO (1989) Prolonged increase in insulin-stimulated glucose transport in muscle after exercise. Am J Physiol 256, E494–E499.Google Scholar
Chomczynski, P & Sacchi, N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156–159.Google Scholar
Danforth, WH (1965) Glycogen synthetase activity in skeletal muscle. Interconversion of two forms and control of glycogen synthesis. J Biol Chem 240, 588–593.Google Scholar
de la, Iglesia, N, Mukhtar, M, Seoane, J, Guinovart, Agius, JJL (2000) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the hepatocyte. J Biol Chem 275, 10597–10603.Google Scholar
Devlin, JT, Hirshman, M, Horton, ED & Horton, ES (1987) Enhanced peripheral and splanchnic insulin sensitivity in NIDDM men after single bout of exercise. Diabetes 36, 434–439.Google Scholar
Grossman, S, Dorn, C & Potter, V (1974) The preparation and characterization of pure rat liver glucokinase. J Biol Chem 249, 3055–3060.Google Scholar
Harada, K, Saheki, S, Wada, K & Tanaka, T (1978) Purification of four pyruvate kinase isozymes of rats by affinity elution chromatography. Biochim Biophys Acta 524, 327–339.Google Scholar
Heine, WE, Klein, PD & Reeds, PJ (1991) The importance of alpha-lactalbumin in infant nutrition. J Nutr 121, 277–283.Google Scholar
Hermansen, L, Hultman, E & Saltin, B (1967) Muscle glycogen during prolonged severe exercise. Acta Physiol Scand 71, 129–139.Google Scholar
Holness, MJ & Sugden, MC (1989) Pyruvate dehydrogenase activities during the fed-to-starved transition and on re-feeding after acute or prolonged starvation. Biochem J 258, 529–533.Google Scholar
Ivy, JL, Goforth, HW, Jr, Damon, BM, McCauley, TR, Parsons, Price, ECTB (2002) Early postexercise muscle glycogen recovery is enhanced with a carbohydrate–protein supplement. J Appl Physiol 93, 1337–1344.Google Scholar
Ivy, JL & Kuo, CH (1998) Regulation of GLUT4 protein and glycogen synthase during muscle glycogen synthesis after exercise. Acta Physiol Scand 162, 295–304.Google Scholar
Karadsheh, NS, Uyeda, K & Oliver, RM (1977) Studies on structure of human erythrocyte phosphofructokinase. J Biol Chem 252, 3515–3524.Google Scholar
Karlsson, J & Saltin, B (1971) Diet, muscle glycogen, and endurance performance. J Appl Physiol 31, 203–206.Google Scholar
Kuo, CH, Browning, KS & Ivy, JL (1999) Regulation of GLUT4 protein expression and glycogen storage after prolonged exercise. Acta Physiol Scand 165, 193–201.Google Scholar
Levine, R & Haft, DE (1970) Carbohydrate homeostasis. I. N Engl J Med 283, 175–183.Google Scholar
Lo, S, Russell, JC & Taylor, AW (1970) Determination of glycogen in small tissue samples. J Appl Physiol 28, 234–236.Google Scholar
McCoy, M, Proietto, J & Hargreaves, M (1996) Skeletal muscle GLUT-4 and postexercise muscle glycogen storage in humans. J Appl Physiol 80, 411–415.Google Scholar
Miwa, I, Okudo, J, Maeda, K & Okuda, G (1972) Mutarotase effect on colorimetric determination of blood glucose with d -glucose oxidase. Clin Chim Acta 37, 538–540.Google Scholar
Morifuji, M, Sakai, K & Sugiura, K (2005) Dietary whey protein modulates liver glycogen level and glycoregulatory enzyme activities in exercise-trained rats. Exp Biol Med 230, 23–30.Google Scholar
Neufer, PD & Dohm, GL (1993) Exercise induces a transient increase in transcription of the GLUT-4 gene in skeletal muscle. Am J Physiol 265, C1597–C1603.Google Scholar
O'Doherty, RM, Bracy, DP, Granner, DK & Wasserman, DH (1996) Transcription of the rat skeletal muscle hexokinase II gene is increased by acute exercise. J Appl Physiol 81, 789–793.Google Scholar
O'Doherty, RM, Bracy, DP, Osawa, H, Wasserman, DH & Granner, DK (1994) Rat skeletal muscle hexokinase II mRNA and activity are increased by a single bout of acute exercise. Am J Physiol 266, E171–E178.Google Scholar
Peyrollier, K, Hajduch, E, Blair, AS, Hyde, R & Hundal, HS (2000) l -leucine availability regulates phosphatidylinositol 3-kinase, p70 S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammalian target of rapamycin (mTOR) pathway in the l -leucine-induced up-regulation of system A amino acid transport. Biochem J 350 Pt 2 361–368.Google Scholar
Phillips, SM, Han, XX, Green, HJ & Bonen, A (1996) Increments in skeletal muscle GLUT-1 and GLUT-4 after endurance training in humans. Am J Physiol 270, E456–E462.Google Scholar
Reeves, PG, Nielsen, FH, Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.Google Scholar
Ren, JM, Semenkovich, CF, Gulve, EA, Gao, J & Holloszy, JO (1994) Exercise induces rapid increases in GLUT4 expression, glucose transport capacity, and insulin-stimulated glycogen storage in muscle. J Biol Chem 269, 14396–14401.Google Scholar
Richter, EA, Garetto, LP, Goodman, MN & Ruderman, NB (1982) Muscle glucose metabolism following exercise in the rat: increased sensitivity to insulin. J Clin Invest 69, 785–793.Google Scholar
Shepherd, PR & Kahn, BB (1999) Glucose transporters and insulin action – implications for insulin resistance and diabetes mellitus. N Engl J Med 341, 248–257.Google Scholar
Smith, PK, Krohn, RI, Hermanson, GT, Mallia, AK, Gartner, FH, Provenzano, MD, Fujimoto, EK, Goeke, NM, Olson, BJ & Klenk, DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150, 76–85.Google Scholar
Terjung, RL, Baldwin, KM, Winder, WW & Holloszy, JO (1974) Glycogen repletion in different types of muscle and in liver after exhausting exercise. Am J Physiol 226, 1387–1391.Google Scholar
Zawadzki, KM, Yaspelkis, BB 3rd Ivy JL (1992) Carbohydrate–protein complex increases the rate of muscle glycogen storage after exercise. J Appl Physiol 72, 1854–1859.Google Scholar