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Dietary protein level alters oxidative phosphorylation in heart and liver mitochondria of chicks

Published online by Cambridge University Press:  09 March 2007

Masaaki Toyomizu ,
Daisuke Kirihara ,
Masahiro Tanaka ,
Kunioki Hayashi  and
Yuichiro Tomita
Masaaki Toyomizu
Affiliation:
Animal Nutrition, Department of Animal Science, Kagoshima University, Korimoto, Kagoshima 890, Japan
Daisuke Kirihara
Affiliation:
Animal Nutrition, Department of Animal Science, Kagoshima University, Korimoto, Kagoshima 890, Japan
Masahiro Tanaka
Affiliation:
Animal Nutrition, Department of Animal Science, Kagoshima University, Korimoto, Kagoshima 890, Japan
Kunioki Hayashi
Affiliation:
Animal Nutrition, Department of Animal Science, Kagoshima University, Korimoto, Kagoshima 890, Japan
Yuichiro Tomita
Affiliation:
Animal Nutrition, Department of Animal Science, Kagoshima University, Korimoto, Kagoshima 890, Japan
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Abstract

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To determine the effects of dietary protein level on cardiac and hepatic mitochondrial oxidative phosphorylation, chicks were fed on semi-purified diets of different protein levels (7, 25, 43 and 61% of metabolizable energy content) for 7, 14 and 21 d. All diets were formulated to contain equivalent fat, mineral and vitamin contents on a gross energy basis. Cardiac and hepatic mitochondrial oxidative phosphorylation rates were assessed polarographically with pyruvate and malate as substrates. Cardiac mitochondria isolated from chicks fed on a 43 or 61% protein-energy diet for 7 d exhibited significantly reduced ADP:oxygen (ADP:O) ratios when compared with mitochondria isolated from chicks fed on a lower-protein-energy diet. Feeding low- (7%) protein-energy diets for 14 d resulted in a relatively increased ADP:O ratio in the heart. Responses of ADP:O ratios to protein level in hepatic mitochondria showed more dependency on protein level than in heart muscle; at all feeding periods the ADP:O ratio decreased with an increase in protein level. As a result, ATP synthesized in the liver, expressed as nmol/mg mitochondrial protein per min, significantly decreased with increased dietary protein level. A parallel correlation was observed, in chicks fed on diets with different levels of protein, between ADP:O ratio for liver mitochondria and body fat. These results suggest that the reduction in oxidative phosphorylation in the heart and liver of animals fed on a higher protein-energy diet may partly contribute to the depression of body fat.

Keywords

Dietary proteinOxidative phosphorylationLiver and heart mitochondriaBody fatChicken
Type
Protein Metabolism
Information
British Journal of Nutrition , Volume 68 , Issue 1 , July 1992 , pp. 89 - 99
Copyright
Copyright © The Nutrition Society 1992

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