Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-25T01:18:50.574Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary modifications of the biliary bile acid glycine: taurine ratio and activity of hepatic bile acid-CoA:amino acid N-acyltransferase (EC 2.3.1) in the rat

Published online by Cambridge University Press:  09 March 2007

T. Ide ,
S. Kano ,
M. Murata ,
T. Yanagita  and
M. Sugano
T. Ide
Affiliation:
Laboratory of Nutritional Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba 305, Japan
S. Kano
Affiliation:
Laboratory of Nutritional Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba 305, Japan
M. Murata
Affiliation:
Laboratory of Nutritional Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba 305, Japan
T. Yanagita
Affiliation:
Laboratory of Nutritional Biochemistry, Saga University School of Agriculture, Saga 840, Japan
M. Sugano
Affiliation:
Laboratory of Food Science, Kyushu University School of Agriculture, Fukuoka 812, Japan
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Effects of dietary manipulations on the biliary bile acid glycine: taurine (G: T) ratio and the activity of hepatic bile acid-Co A: amino acidN-acyltransferase (EC 2.3.1) in the post-mitochondrial fraction of liver homogenates were examined in the rat. The G:T ratio in rats fed on the diet containing 100 g pectin/kg (2.18) was markedly higher than that in the animals fed on the diet containing 100 g cellulose/kg (0.09). The diets containing either 10 g cholesterol/kg or 5 g sodium cholate/kg, especially the latter, also increased the G:T ratio (0.77 and 2.33 respectively) compared with a control diet free of these steroids (0.34). When the saturating concentrations of taurine (20 mM) and glycine (100 mM) were the substrates, dietary pectin relative to cellulose significantly increased the activity of both taurine- and glycine-dependent bile acid-CoA: amino acidN-acyltransferase, but neither dietary bile acid nor cholesterol influenced it. In spite of the marked difference in the G:T ratio among the rats given various types of experimental diet, the bile acid-CoA: amino acid N-acyltransferase reaction produced taurine- but little glycine-conjugated bile acid when both taurine and glycine coexisted at physiological concentration ranges in the assay media. Dietary manipulations modified the hepatic taurine concentrations and the changes were inversely correlated with those in the G: T ratio. However, hepatic concentration of taurine (1.67–4.82 μmol/g) in rats given various types of experimental diet was comparable with or even higher than the reported Michaelis constant (Km) value ofN-acyltransferase for this compound (0.8–2.5 mM). In contrast, glycine concentrations (1.81–2.58 μmol/g) were much lower than the Km value for this amino acid (35–40 mM) under various dietary conditions. Thus, neither the substrate specificity of the bile acid conjugation enzyme nor the alteration in the hepatic concentration of taurine or glycine accounted for the change in the G: T ratio in the present study.

Keywords

Bile acid conjugationDietary fibreCholesterolRat
Type
Dietary modfification of bile acvid secretion
Information
British Journal of Nutrition , Volume 72 , Issue 1 , July 1994 , pp. 93 - 100
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

American Institute of Nutrition (1977). Report of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Bremer, J. (1955). Choloyl-S-CoA as an intermediate in the conjugation of cholic acid with taurine by rat liver microsomes. Acta Chemica Scandinavica 10, 56–71.CrossRefGoogle Scholar
Cummings, J. H., Englyst, H. N. & Wiggins, H. S. (1986). The role of carbohydrates in lower gut function. Nutrition Reviews 44, 50–54.Google ScholarPubMed
Czuba, B. & Vessey, D. A. (1981 a). Purification and characterization of choloyl-CoA: taurine N-acyltransferase from the liver of domestic fowl (Gallus gallus). Biochemical Journal 195, 263–266.CrossRefGoogle Scholar
Czuba, B. & Vessey, D. A. (1981 b). Identification of a unique mammalian species of cholyl-CoA:amino acid N- acyltransferase. Biochimica et Biophysica Acta 665, 612–614.CrossRefGoogle ScholarPubMed
Elliott, W. E. (1984). Metabolism of bile acid in liver and extrahepatic tissues. In Sterols and Bile Acids, pp. 303–329 [Danielsson, H. and Sjovall, J., editors]. Amsterdam: Elsevier Science Publishers B.V.Google Scholar
Hardison, W. G. M. & Proffitt, J. H. (1977). Influence of hepatic taurine concentration on bile acid conjugation withtaurine. American Journal of Physiology 232, E75–E79.Google Scholar
Haslewood, G. A. D. & Wooton, V. (1950). Comparative studies of ‘bile salts’. 1. Preliminary survey. Biochemical Journal 47, 584–597.CrossRefGoogle Scholar
Ide, T. & Horii, M. (1987). A simple method for the extraction and determination of non-conjugated and conjugated luminal bile acids in rats. Agricultural and Biological Chemistry 51, 3155–3157.Google Scholar
Ide, T. & Horii, M. (1989). Predominant conjugation with glycine of biliary and lumen bile acids in rats fed on pectin. British Journal of Nutrition 61, 545–557.CrossRefGoogle ScholarPubMed
Ide, T., Horii, M., Kawashima, K. & Yamamoto, T. (1989). Bile acid conjugation and hepatic taurine concentration in rats fed on pectin. British Journal of Nutrition 62, 539–550.CrossRefGoogle ScholarPubMed
Ide, T., Horii, M., Yamamoto, T. & Kawashima, K. (1990). Contrasting effects of water-soluble and water- insoluble dietary fibers on bile acid conjugation and taurine metabolism in the rat. Lipids 25, 335–340.CrossRefGoogle ScholarPubMed
Ide, T. & Sugano, M. (1991). Interaction of dietary protein differing in sulfur amino acid content and pectin on bile acid conjugation in immature and mature rats. Journal of Nutrition 121, 985–993.CrossRefGoogle ScholarPubMed
Jacobsen, J. G. & Smith, L. H. Jr. (1968). Biochemistry and physiology of taurine and taurine derivatives. Physiological Review 48, 424–511.CrossRefGoogle ScholarPubMed
Johnson, M. R., Barnes, S., Kwakye, J. B. & Diasio, R. B. (1991). Purification and characterization of bile acid-CoA: amino acid N-acyltransferase from human liver. Journal of Biological Chemistry 266, 10227–10233.CrossRefGoogle ScholarPubMed
Kase, B. F. & Bjorkhem, I. (1989). Peroxisomal bile acid-CoA:amino acid N-acyltransferase in rat liver. Journal of Biological Chemistry 264, 9220–9223.CrossRefGoogle ScholarPubMed
Kase, B. F., Prydz, K., Bjorkhem, I. & Pedersen, J. I. (1986). Conjugation of cholic acid with taurine and glycine by rat liver peroxisomes. Biochemical and Biophysical Research Communications 138, 167–173.CrossRefGoogle ScholarPubMed
Killenberg, P. G. (1978). Measurement and subcellular distribution of choloyl-CoA synthetase and bile acid-CoA:amino acid N-acyltransferase activities in rat liver. Journal of Lipid Research 19, 24–31.CrossRefGoogle ScholarPubMed
Killenberg, P. G. & Jordan, J. T. (1978). Purification and characterization of bile acid-CoA:amino acid N- acyltransferase from rat liver. Journal of Biological Chemistry 253, 1005–1010.CrossRefGoogle ScholarPubMed
Kwakye, J. B., Johnson, M. R., Barnes, S. & Diasio, R. B. (1991). A comparative study of bile acid CoA:amino acid: N-acyltransferase (BAT) from four mammalian species. Comparative Biochemistry and Physiology 100B,131–136Google Scholar
Myant, N. B. & Mitropoulos, K. A. (1977). Cholesterol 7a-hydroxylase. Journal of Lipid Research 18, 135–153.CrossRefGoogle Scholar
Ogura, Y. & Ogura, M. (1986). Dynamics of the conjugate pattern during the infusion of bile acids into isolated rat liver. Biological Chemistry Hoppe-Seyler 367,495–500.CrossRefGoogle ScholarPubMed
Scherstkn, T., Bjomtorp, P., Edkahl, P-H. & Bjorkerud, S. (1967). The synthesis of taurocholic and glycocholic acids by preparations of human liver. II. An analysis of the stimulating effect of the L fraction. Biochimica et Biophysica Acta 141, 151–163.Google Scholar
Shah, P. P. & Staple, E. (1967). Synthesis of coenzyme A esters of some bile acids. Steroids 12, 571–576.CrossRefGoogle Scholar
Snedecor, G. W. & Cochran, W. G. (1989) Statistical Methods, 8th ed. Ames: Iowa State University Press.Google Scholar
Sweeny, D. J., Stephan, B. & Diasio, R. B. (1991). Bile acid conjugation pattern in the isolated perfused rat liver during infusion of an amino acid formulation. Journal of Parenteral and Enteral Nutrition 15, 303–306.CrossRefGoogle ScholarPubMed
Vessey, D. A. (1978). The biochemical basis for the conjugation of bile acid either with glycine or taurine. Biochemical Journal 174, 621–626.CrossRefGoogle ScholarPubMed
Vessey, D. A. (1979). The co-purification and common identity of cholyl CoA:glycine and cholyl CoA: taurine- N-acyltransferase activities from bovine liver. Journal of Biological Chemistry 254, 2059–2063.CrossRefGoogle ScholarPubMed
Vessey, D. A., Benfatto, A. M., Zerweck, E. & Vestweber, C. (1990). Purification and characterization of the enzymes of bile acid conjugation from fish liver. Comparative Biochemistry and Physiology 95B, 647–652.Google Scholar
Vessey, D. A., Crissey, M. H. & Zakim, D. (1977). Kinetic studies on the enzymes conjugating bile acids with taurine and glycine in bovine liver. Biochemical Journal 163, 181–183.CrossRefGoogle ScholarPubMed
Zang, R., Barnes, S. & Diasio, R. B. (1992). Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats. American Journal of Physiology 262, G351–358.Google Scholar