Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-15T09:19:39.222Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary linoleic acid at high and reduced dietary fat level decreases the faecal excretion of vitamin E in young rats

Published online by Cambridge University Press:  09 March 2007

Lilian B. M. Tuburg ,
Edward Haddeman ,
Gerard A. A. Kivits ,
Jan A. Weststrate  and
Elizabeth J. Brink
Lilian B. M. Tuburg
Affiliation:
Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands
Edward Haddeman
Affiliation:
Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands
Gerard A. A. Kivits
Affiliation:
Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands
Jan A. Weststrate
Affiliation:
Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands
Elizabeth J. Brink
Affiliation:
Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Vitamin E is the major lipid-soluble antioxidant in human subjects and is crucial in protecting polyunsaturated fatty acids (PUFA) against lipid peroxidation. Dietary PUFA have been suggested to inhibit the absorption of vitamin E. The present study in young male rats was designed to investigate the effect of increasing concentrations of dietary linoleic acid on the faecal excretion of vitamin E. The rats were fed on semi-synthetic diets containing two concentrations of fat (59 g/kg diet, 15 energy % (en%) or 131 g/kg, 30 en%) for 3 weeks. Triacylglycerol rich in linoleic acid was added at the expense of triacylglycerol rich in saturated fatty acids to obtain dietary concentrations of 13,39 or 66 g linoleic acid/kg diet for the high-fat diet (131 g fat/kg) and 12, 24 or 36 g linoleic acid/kg diet for the reduced-fat diet (59 g fat/kg). The results from the present study demonstrate that the faecal excretion of vitamin E was significantly lower in rats fed on diets with high levels of linoleic acid compared with rats fed on lower levels of linoleic acid irrespective of the dietary fat content. The concentration of vitamin E in liver and plasma was significantly lower in animals fed on the highest concentration of linoleic acid compared with those fed on the lowest level. Results from the present study also demonstrate that at the same concentration of linoleic acid, the faecal excretion of vitamin E in rats fed on reduced-fat diets was significantly lower than in rats fed on high-fat diets. Our findings indicate that the apparent absorption of vitamin E is not inhibited by dietary PUFA. Results from the present study also demonstrate that a reduction of dietary fat content from 30 en% to 15 en% does not lower the apparent absorption of vitamin E

Keywords

Linoleic acidVitamin ERats
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 2 , February 1997 , pp. 327 - 336
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Ames, B. N. (1983). Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 221, 1256–1264.CrossRefGoogle ScholarPubMed
Björneboe, A., Björneboe, G. A. & Drevon, C. A. (1990). Absorption, transport and distribution of vitamin E. Journal of Nutrition 120, 233–242.CrossRefGoogle ScholarPubMed
Brink, E. J., Haddeman, E., De Fouw, N. J. & Weststrate, J. A. (1995). Positional distribution of stearic acid and oleic acid in a triacylglycerol and dietary calcium concentration determines the apparent absorption of these fatty acids in rats. Journal of Nutrition 125, 2379–2387.CrossRefGoogle Scholar
Burton, G. W. (1990). Vitamin E: antioxidant activity, biokinetics and bioavailability. Annual Review of Nutrition 10, 357–382.CrossRefGoogle ScholarPubMed
Catignani, G. L. (1986). An HPLC method for the simultaneous determination of retinol and α-tocopherol in plasma or serum. Methods in Enzymology 123, 215–219.CrossRefGoogle ScholarPubMed
Dougherty, R. M., Allman, M. A. & Iacono, J. M. (1995). Effects of diets containing high or low amounts of stearic acid on plasma lipoprotein fractions and fecal fatty acid excretion of men. American Journal of Clinical Nutrition 61, 1120–1128.CrossRefGoogle ScholarPubMed
Farwer, S. R., Boer, B. C. J., de, Haddeman, E., Kivits, G. A. A., Wiersma, A. & Danse, B. H. J. C. (1994). The vitamin E nutritional status of rats fed on diets high in fish oil, linseed oil or sunflowerseed oil. British Journal of Nutrition 72, 127–145.CrossRefGoogle ScholarPubMed
Gallo-Torres, H. E., Weber, F. & Wiss, O. (1971). The effect of different dietary lipids on the lymphatic appearance of vitamin E. International Journal of Vitamin & Nutrition Research 41, 504–515.Google ScholarPubMed
Green, J., Diplock, A. T., Bunyan, J., McHale, D. & Muthy, I. R. (1967). Dietary unsaturated fatty acid stress and the metabolism of a-tocopherol in the rat. British Journal of Nutrition 21, 69–101.CrossRefGoogle Scholar
Horwitt, M. K. (1974). Status of human requirement for vitamin E. American Journal of Clinical Nutrition 27, 1182–1193.CrossRefGoogle ScholarPubMed
Horwitt, M. K., Harvey, C. C., Dahm, D. H. & Searcy, M. T. (1972). Relationship between tocopherol and serum livid levels for determination of nutritional adequacy. Annals of the New York Academy of Sciences 203, 223–236.CrossRefGoogle Scholar
Jager, F. C. & Houtsmuller, U. M. T. (1970). Effect of dietary linoleic acid on vitamin E requirement and fatty acid composition of erythrocyte liuids in rats. Nutrition and Metabolism 12, 3–12.CrossRefGoogle ScholarPubMed
Kayden, H. J. & Traber, M. G. (1993). Absorption, lipoprotein transport, and regulation of plasma concentrations of vitamin E in humans. Journal of Lipid Research 34, 343–358.CrossRefGoogle ScholarPubMed
Kuksis, A. (editor) (1987). Absorption of fat soluble vitamins. In Fat Absorption, pp. 65–86. Boca Raton, FL: CRC Press Inc.Google Scholar
Mouri, K., Ikesu, H., Ekasa, T. & Igarashi, O. (1984). The influence of marine oil intake upon levels of lipids, a-tocopherol and lipid peroxidation in serum and liver of rats. Journal of Nutritional Science and Vitaminology 30, 307–318.CrossRefGoogle Scholar
Statistical Analysis Systems (1987). SAS/STAT Guide for Personal Computers, 6th ed. Cary,NC: SAS Institute Inc.Google Scholar
Steinberg, D., Parthasarathy, S., Carew, T. E., Khoo, J. C. & Witzum, J. L. (1989). Beyond cholesterol. Modifications of low density lipoprotein that increase its atherogenicity. New England Journal of Medicine 320, 915–924.Google ScholarPubMed
Traber, M. G., Cohn, W. & Muller, D. P. R. (1993). Absorption, transport and delivery to tissues. In Vitamin E in Health and Disease, pp. 35–51 [Packer, L. & Fuchs, J., editors]. New York: Marcel Dekker Inc.Google Scholar
Weber, F., Weiser, H. & Wiss, O. (1964). Bedarf an Vitamin E in Abhängigkeit von der zufuhr an Linolsäure (Vitamin E requirement as a function of linoleic acid supply). Zeitschrift fur Ernährungswissenschaft 4, 245–253.CrossRefGoogle Scholar