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A diet rich in phosphatidylethanolamine increases plasma homocysteine in mink: a comparison with a soyabean oil diet

Published online by Cambridge University Press:  08 March 2007

Hanne Müller ,
Terje Grande ,
Øystein Ahlstrøm  and
Anders Skrede
Hanne Müller*
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway
Terje Grande
Affiliation:
University College of Akershus, PO Box 423, 2001 Lillestrøm, Norway
Øystein Ahlstrøm
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway
Anders Skrede
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway Aquaculture Protein Centre, Centre of Excellence, PO Box 5003, N-1432 Ås, Norway
*
*Corresponding author: Dr Hanne Müller, fax +47 64965101, email [email protected]
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Abstract

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The effects of high dietary levels of phosphatidylethanolamine (PE) on plasma concentrations of homocysteine (tHcy) have not previously been studied. Eighteen mink (Mustela vison) studied were fed one of three diets during a 25d period in a parallel-group design. The compared diets had 0, 17 and 67% extracted lipids from natural gas-utilising bacteria (LNGB), which were rich in PE. The group with 0% LNGB was fed a diet of 100% soyabean oil (SB diet). Phospholipids are the main lipid components in LNGB and Methylococcus capsulatus is the main bacteria (90%). The fasting plasma concentration of tHcy was significantly higher when the mink consumed the diet with 67% LNGB than when they consumed the SB diet (P=0·039). A significantly lower glutathione peroxidase activity was observed in mink consuming the 17% LNGB diet or the 67% LNGB diet than was observed in mink fed the SB diet. The lack of significant differences in the level of plasma PE due to the diets indicates that most of the PE from the 67% LNGB diet was converted to phosphatidylcholine (PC) in the liver. It has previously been hypothesised that phosphatidylethanolamine N-methyltransferase is an important source of tHcy. The present results indicate that plasma tHcy is at least partly regulated by phospholipid methylation from PE to PC. This methylation reaction is a regulator of physiological importance.

Keywords

HomocysteinePhosphatidylethanolamineDietNatural gas-utilising bacteria
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 684 - 690
Copyright
Copyright © The Nutrition Society 2005

References

Agellon, LB, Walkey, CJ, Vance, DE, Kuipers, F & Verkade, HJ (1999) The unique acyl chain specificity of biliary phosphatidylcholines in mice is independent of their biosynthetic origin in the liver. Hepatology 30, 725–729.CrossRefGoogle ScholarPubMed
Åkesson, B (1982) Content of phospholipids in human diets studied by the duplicate-portion technique. Br J Nutr 47, 223–229.CrossRefGoogle ScholarPubMed
Bleich, S, Carl, M, Bayerlein, K, Reulbach, U, Biermann, T, Hillemacher, T, Bonsch, D & Kornhuber, J (2005) Evidence of increased homocysteine levels in alcoholism: the Franconian alcoholism research studies (FARS). Alcohol Clin Exp Res 29, 334–336.CrossRefGoogle ScholarPubMed
Bligh, EG & Dyer, WJ (1959) A rapid method of total lipid extraction and purification. Can J Med Sci 37, 911–917.Google ScholarPubMed
Buchman, AL, Dubin, M, Jenden, D, Moukarzel, A, Roch, MH, Rice, K, Gornbein, J, Ament, ME & Eckhert, CD (1992) Lecithin increases plasma free choline and decreases hepatic steatosis in long-term total parenteral nutrition patients. Gastroenterol 102, 1363–1370.CrossRefGoogle ScholarPubMed
Carrasco, MP, Sanchez-Amate, MC, Segovia, JL & Marco, C (1996) Studies on phospholipid biosynthesis in hepatocytes from alcoholic rats by using radiolabeled exogenous precursors. Lipids 31, 393–397.CrossRefGoogle ScholarPubMed
Chiang, PK, Gordon, RK, Tal, J, Zeng, GC, Doctor, BP, Pardhasaradhi, K & McCann, PP (1996) S-Adenosylmethionine and methylation. FASEB J 10, 471–480.CrossRefGoogle ScholarPubMed
Cui, Z, Vance, JE, Chen, MH, Voelker, DR & Vance, DE (1993) Cloning and expression of a novel phosphatidylethanolamine N-methyltransferase. A specific biochemical and cytological marker for a unique membrane fraction in rat liver. J Biol Chem 268, 16655–16663.CrossRefGoogle ScholarPubMed
DeLong, CJ, Hicks, AM & Cui, Z (2002) Disruption of choline methyl group donation for phosphatidylethanolamine methylation in hepatocarcinoma cells. J Biol Chem 277, 17217–17225.CrossRefGoogle ScholarPubMed
Finkelstein, JD (1990) Methionine metabolism in mammals. J Nutr Biochem 1, 228–237.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497–509.CrossRefGoogle ScholarPubMed
Houweling, M, Cui, Z, Tessitore, L & Vance, DE (1997) Induction of hepatocyte proliferation after partial hepatectomy is accompanied by a markedly reduced expression of phosphatidylethanolamine N-methyltransferase-2. Biochim Biophys Acta 1346, 1–9.CrossRefGoogle ScholarPubMed
Kent, C & Carman, GM (1999) Interactions among pathways for phosphatidylcholine metabolism, CTP synthesis and secretion through the Golgi apparatus. Trends Biochem Sci 24, 146–150.CrossRefGoogle ScholarPubMed
Loehrer, FM, Angst, CP, Haefeli, WE, Jordan, PP, Ritz, R & Fowler, B (1996) Low whole-blood S-adenosylmethionine and correlation between 5-methyltetrahydrofolate and homocysteine in coronary artery disease. Arterioscler Thromb Vasc Biol 16, 727–733.CrossRefGoogle ScholarPubMed
McCleary, BV, Solah, V & Gibson, TS (1994) Quantitative measurements of total starch in cereal flours and products. J Cereal Sci 20, 51–58.CrossRefGoogle Scholar
Makula, RA (1978) Phospholipid composition of methane-utilizing bacteria. J Bacteriol 134, 771–777.CrossRefGoogle ScholarPubMed
Martins, PJ, Galdieri, LC, Souza, FG, Andersen, ML, Benedito-Silva, AA, Tufik, S & D'Almeida, V (2005) Physiological variation in plasma total homocysteine concentrations in rats. Life Sci 76, 2621–2629.CrossRefGoogle ScholarPubMed
Mattson, MP & Haberman, F (2003) Folate and homocysteine metabolism: therapeutic targets in cardiovascular and neurodegenerative disorders. Curr Med Chem 10, 1923–1929.CrossRefGoogle ScholarPubMed
Miller, JW, Nadeau, MR, Smith, J, Smith, D & Selhub, J (1994) Folate-deficiency-induced homocysteinaemia in rats: disruption of S-adenosylmethionine's co-ordinate regulation of homocysteine metabolism. Biochem J 298, 415–419.CrossRefGoogle ScholarPubMed
Mudd, SH, Finkelstein, JD & Refsum, H (2000) Homocysteine and its disulfide derivatives: a suggested consensus terminology. Arterioscler Thromb Vasc Biol 20, 1704–1706.CrossRefGoogle ScholarPubMed
Müller, H, Hellgren, LI, Olsen, E & Skrede, A (2004) Lipids rich in phosphatidylethanolamine from natural gas-utilizing bacteria reduce plasma cholesterol and classes of phospholipids: a comparison with soybean oil. Lipids 39, 833–841.CrossRefGoogle Scholar
National Research Council (1982) Nutrient Requirements of Mink and Foxes, 2nd revised ed. Washington, DC: National Academy Press.Google Scholar
Noga, AA, Stead, LM, Zhao, Y, Brosnan, ME, Brosnan, JT & Vance, DE (2003) Plasma homocysteine is regulated by phospholipid methylation. J Biol Chem 278, 5952–5959.CrossRefGoogle ScholarPubMed
Noga, AA & Vance, DE (2003) A gender-specific role for phosphatidylethanolamine N-methyltransferase-derived phosphatidylcholine in the regulation of plasma high density and very low density lipoproteins in mice. J Biol Chem 278, 21851–21859.CrossRefGoogle ScholarPubMed
Olthof, MR & Verhoef, P (2005) Effects of betaine intake on plasma homocysteine concentrations and consequences for health. Curr Drug Metab 6, 15–22.CrossRefGoogle ScholarPubMed
Paglia, DE & Valentine, WN (1967) Studies on the quantitative and qualitative characteristics of the erythrocyte gluthatione peroxidase. J Lab Clin Med 70, 158–169.Google Scholar
Panagiotakos, DB, Pitsaros, C, Zampelas, A, Zeimbekis, A, Chyrsohoou, C, Papademetriou, L & Stefanadis, C (2004) The association between coffee consumption and plasma total homocysteine levels: the "ATTICA" study. Heart Vessels 19, 280–286.Google ScholarPubMed
Ridgway, ND, Uao, Z & Vance, DE (1989) Phosphatidylethanolamine levels and regulation of phosphatidylethanolamine N-methyltransferase. J Biol Chem 264, 1203–1207.CrossRefGoogle ScholarPubMed
Rossi, E, Beilby, JP, McQuillan, BM & Hung, J (1999) Biological variability and reference intervals for total plasma homocysteine. Ann Clin Biochem 36, 56–61.CrossRefGoogle ScholarPubMed
Sehayek, E, Wang, R, Ono, JG, Zinchuk, VS, Duncan, EM, Shefer, S, Vance, DE, Ananthanarayanan, M, Chait, BT & Breslow, JL (2003) Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol. J Lipid Res 44, 1605–1613.CrossRefGoogle Scholar
Selhub, J (1999) Homocysteine metabolism. Annu Rev Nutr 19, 217–246.CrossRefGoogle ScholarPubMed
Skrede, A, Berge, GM, Storebakken, T, Herstad, O, Aarstad, K & Sundstøl, F (1998) Digestibility of bacterial protein grown on natural gas in mink, pigs, chicken and Atlantic salmon. Anim Feed Sci Technol 76, 103–116.CrossRefGoogle Scholar
Steenge, GR, Verhoef, P & Katan, MB (2003) Betaine supplementation lowers plasma homocysteine in healthy men and women. J Nutr 133, 1291–1295.CrossRefGoogle ScholarPubMed
Tijburg, LB, Geelen, MJH & Van Golde, LMG (1989) Regulation of the biosynthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine in the liver. Biochem Biophys Acta 1004, 1–19.CrossRefGoogle ScholarPubMed
Upchurch, GR Jr, Welch, GN, Fabian, AJ, Freedman, JE, Johnson, JL, Keaney, JF Jr & Loscalzo, J (1997) Homocyst(e)ine decreases bioavailable nitric oxide by a mechanism involving glutathione peroxidase. J Biol Chem 272, 17012–17017.CrossRefGoogle ScholarPubMed
Verhoef, P, Pasman, WJ, Van Vliet, T, Urgert, R & Katan, MB (2002) Contribution of caffeine to the homocysteine-raising effect of coffee: a randomized controlled trial in humans. Am J Clin Nutr 76, 1244–1248.CrossRefGoogle Scholar
Wurtman, RJ, Hirsch, MJ & Growdon, JH (1979) Lecithin consumption raises serum-free-choline levels. Lancet ii, 68–69.Google Scholar
Yi, P, Melnyk, S, Pogribna, M, Pogribny, IP, Hine, RJ & James, SJ (2000) Increase in plasma homocysteine associated with parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J Biol Chem 275, 29318–29323.CrossRefGoogle ScholarPubMed