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Dexamethasone induces sodium-dependant vitamin C transporter in a mouse osteoblastic cell line MC3T3-E1

Published online by Cambridge University Press:  09 March 2007

I. Fujita ,
J. Hirano ,
N. Itoh ,
T. Nakanishi  and
K. Tanaka
I. Fujita
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
J. Hirano
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
N. Itoh
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
T. Nakanishi
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
K. Tanaka*
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
*
*Corresponding author: Dr K. Tanaka, fax +81 6 6879 8234, email [email protected]
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Abstract

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The regulation of intracellular ascorbic acid (AsA) levels may be under the control of an AsA-specific membrane transporter. The present study investigates AsA uptake and expression of Na-dependent vitamin C transporter (SVCT) mRNA in the mouse osteoblastic cell line, MC3T3-E1. Among eight compounds tested, dexamethasone (Dex) all-trans retinoic acid, transforming growth factor β, prostaglandin E2 and transferrin significantly (P<0·01, P<0·01, P<0·05 and P<0·01 respectively) stimulated the update of AsA into MC3T3-E1 cells. Among these five, Dex was the most active, inducing mSVCT2 mRNA and the uptake of AsA in a time- and concentration-dependant manner. Dex did not induce mSVCT1 mRNA. These results suggest that the Dex-induced stimulation of AsA incorporation into osteoblastic cells is mediated by the induction of mSVCT2. Since Dex reduced alkaline phosphatase activity in MC3T3-E1 cells in our culture conditions, Dex-induced stimulation of AsA incorporation might not be the result of differentiation. Hormone-regulated changes of SVCT expression may have an important role in cell functions.

Keywords

Ascorbic acidTransporterSodium-dependant vitamin C transporterOsteoblastMC3T3-E1Dexamethasone
Type
Short communication
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 145 - 149
Copyright
Copyright © The Nutrition Society 2001

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