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Degradation of transgenic DNA from genetically modified soya and maize in human intestinal simulations

Published online by Cambridge University Press:  09 March 2007

Susana M. Martín-Orúe ,
Anthony G. O'Donnell ,
Joaquin Ariño ,
Trudy Netherwood ,
Harry J. Gilbert  and
John C. Mathers
Susana M. Martín-Orúe
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
Anthony G. O'Donnell
Affiliation:
Department of Agricultural and Environmental Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
Joaquin Ariño
Affiliation:
Departament de Bioquímica i Biologia Molecular & Servei de Seqüenciació de DNA, Universitat Autonoma de Barcelona, Spain
Trudy Netherwood
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
Harry J. Gilbert
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
John C. Mathers*
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
*
*Corresponding author: Professor John C. Mathers, fax +44 191 2228684, email [email protected]
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Abstract

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The inclusion of genetically modified (GM) foods in the human diet has caused considerable debate. There is concern that the transfer of plant-derived transgenes to the resident intestinal microflora could have safety implications. For these gene transfer events to occur, the nucleic acid would need to survive passage through the gastrointestinal tract. The aim of the present study was to evaluate the rate at which transgenes, contained within GM soya and maize, are degraded in gastric and small bowel simulations. The data showed that 80 % of the transgene in naked GM soya DNA was degraded in the gastric simulations, while no degradation of the transgenes contained within GM soya and maize were observed in these acidic conditions. In the small intestinal simulations, transgenes in naked soya DNA were degraded at a similar rate to the material in the soya protein. After incubation for 30 min, the transgenes remaining in soya protein and naked DNA were 52 (SEM 13·1) % AND 34 (sem 17·5) %, respectively, and at the completion of the experiment (3 h) these values were 5 % and 3 %, respectively. In contrast to the soya transgene, the maize nucleic acid was hydrolysed in the small intestinal simulations in a biphasic process in which approximately 85 % was rapidly degraded, while the rest of the DNA was cleaved at a rate similar to that in the soya material. Guar gum and tannic acid, molecules that are known to inhibit digestive enzymes, did not influence the rate of transgene degradation in soya protein. In contrast guar gum reduced the rate of transgene degradation in naked soya DNA in the initial stages, but the polysaccharide did not influence the amount of nucleic acid remaining at the end of the experiment. Tannic acid reduced the rate of DNA degradation throughout the small bowel simulations, with 21 (sem 5·4) % and 2 (sem 1·8) % of the naked soya DNA remaining in the presence and absence of the phenolic acid, respectively. These data indicate that some transgenes in GM foods may survive passage through the small intestine.

Keywords

Genetically modified foodsIntestinal degradationQuantitative polymerase chain reaction
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 6 , June 2002 , pp. 533 - 542
Copyright
Copyright © The Nutrition Society 2002

References

Anai, M, Muta, A, Umeno, M & Sasaki, M (1983) Purification and properties of an acid deoxyribonuclease from rat small intestinal mucosa. Journal of Biochemistry (Tokyo) 94, 339–344.CrossRefGoogle ScholarPubMed
Cameron-Smith, D, Collier, GR & O'Dea, K (1994) Effect of soluble dietary fibre on the viscosity of gastrointestinal contents and the acute glycaemic response in the rat. British Journal of Nutrition 71, 563–571.Google Scholar
Ehrlein, H & Stockmann, A (1998) Absorption of nutrients is only slightly reduced by supplementing enteral formulas with viscous fiber in miniature pigs. Journal of Nutrition 128, 2446–2455.CrossRefGoogle ScholarPubMed
Glahn, RP, Wein, EM, VanCampen, DR & Miller, DD (1996) Caco-2 cell iron uptake from meat and casein digests parallels in vivo studies: Use of a novel in vitro method for rapid estimation of iron bioavailability. Journal of Nutrition 126, 332–339.Google Scholar
Horigome, T, Kumar, R & Okamoto, K (1988) Effect of condensed tannins prepared from leaves of fodder plants on digestive enzymes in vitro and in the intestine of rats. British Journal of Nutrition 60, 275–285.Google Scholar
Hupfer, C, Hotzel, H, Sachse, K & Engel, K-H (1998) Detection of the genetic modification in heat-trated products of Bt maize by polymerase chain reaction. Zeitschrift für Lebensmittel, Untersuchung und Forschung A (Food Research and Technology) 206, 203–207.Google Scholar
KeShun, L (1999) Soybeans, Chemistry Technology and Utilization. Gaithersburg, MD: Aspen Publishers, Inc.Google Scholar
Khan, NS, Ahmad, A & Hadi, SM (2000) Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA. Chemico-Biological Interactions 125, 177–189.CrossRefGoogle ScholarPubMed
Koziel, MG, Beland, GL, Bowman, C, Carozzi, NB, Crenshaw, R, Crossland, L, Dawson, J, Desai, N, Hill, M, Kadwell, S, Launis, K, Lewis, K, Maddox, D, McPherson, K, Meghji, MR, Merlin, E, Rhodes, R, Warren, GW, Wright, M & Evola, SV (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio-Technology 11, 194–200.Google Scholar
McAllan, AB (1980) The degradation of nucleic acids in, and the removal of breakdown products from, the small intestines of steers. British Journal of Nutrition 44, 99–112.CrossRefGoogle ScholarPubMed
Mathers, JC (1991) Digestion of non-starch polysaccharides by non-ruminant omnivores. Proceedings of the Nutrition Society 50, 161–172.CrossRefGoogle ScholarPubMed
Maturin, L & Curtiss, R (1976) Degradation of nucleic acid by nucleases in intestinal tract of rats. Science 196, 216–218.CrossRefGoogle Scholar
Mercer, DK, Scott, KP, Bruce-Johnson, WA, Glover, LA & Flint, HJ (1999) Fate of free DNA and transformation of the oral bacterium Streptococcus gordonii DL1 by plasmid DNA in human saliva. Applied and Environmental Microbiology 65, 6–10.CrossRefGoogle ScholarPubMed
Nagae, S, Nakayama, J, Nakano, I & Anai, M (1982) Purification and properties of a neutral endodeoxyribonuclease from rat small intestinal mucosa. Biochemistry 21, 1339–1344.CrossRefGoogle ScholarPubMed
Nakayama, J, Fujiyoshi, T, Nakamura, M & Anai, M (1981) Purification and properties of an endodeoxyribonuclease from nuclei of bovine small intestinal mucosa. Journal of Biological Chemistry 256, 1636–1642.Google Scholar
Padgette, SR, Kolacz, KH, Delannay, X, Re, DB, Lavallee, BJ, Tinius, CN, Rhodes, WK, Otero, YI, Barry, GF, Eichholtz, DA, Peschke, VM, Nida, DL, Taylor, NB & Kishore, GM (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Science 35, 1451–1461.Google Scholar
Quesada, C, Bartolomé, B, Nieto, O, Gómez-Cordovés, C, Hernández, T & Estrella, I (1996) Phenolic inhibitors of α -amylase and trypsin enzymes by extracts from pears, lentils and cocoa. Journal of Food Protection 59, 185–192.Google Scholar
Raeymaekers, L (1993) QuantitativePCR: theoretical considerations with practical implications. Analytical Biochemistry 214, 582–585.Google Scholar
Raw, JD (1983) Biochemistry. p. 358. New York: Harper and Row Publishers, Inc.Google Scholar
SAS Institute (1996) SAS®. Cary, NC: SAS Institute> Inc.+Inc.>Google Scholar
Schubbert, R, Lettmann, C & Doerfler, W (1994) Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice. Molecular and General Genetics 242, 495–504.CrossRefGoogle ScholarPubMed
Schubbert, R, Renz, D, Schmitz, B & Doerfler, W (1997) Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proceedings of the National Academy of Sciences of the United States of America 94, 961–966.CrossRefGoogle ScholarPubMed
Sokal, RR & Rohlf, FJ (1995) Biometry, the Principles and Practice of Statistics in Biological Research. New York: Freeman.Google Scholar
Studer, E, Rhyner, C, Lüthy, J & Hübner, P (1998) Quantitative competitive PCR for the detection of genetically modified soybean and maize. Zeitschrift für Lebensmitte, Untersuchung und Forschung A (Food Research and Technology) 207, 207–213.CrossRefGoogle Scholar