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Degradation of glucosinolates during in vitro incubations of rapeseed meal with myrosinase (EC 3.2.3 1) and with pepsin (EC 3.4.23.l)–hydrochloric acid, and contents of porcine small intestine and caecum

Published online by Cambridge University Press:  09 March 2007

Ian Maskell  and
Ron Smithard
Ian Maskell
Affiliation:
Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEI 7RU
Ron Smithard
Affiliation:
Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEI 7RU
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Abstract

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Changes in the concentrations of glucosinolates from rapeseed meal and some glucosinolate degradation products during incubation in vitro with myrosinase (EC 3.2.3.1), with pepsin (EC 3.4.23.1)–HCI, and with contents of porcine small intestine and caecum were studied. When rapeseed meal was incubated with myrosinase, 5-vinyl oxazolidinethione (OZT) and butenyl and pentenyl isothiocyanates were produced; OZT concentration rose to a plateau after about 2 h. However, when incubated with caecal contents only OZT could be detected; its concentration peaked after about 4–5 h then declined. Under in vitro conditions whirh attempted to simulate peptic and small intestinal digestion no OZT could be detected; the individual glucosinolates differed in susceptibility to peptic conditions, losses ranging from 3 to 23%. Under the small intestinal conditions the losses of individual glucosinolates ranged from about 7 to 28%. Addition of CuSO4, ascorbic acid, tylosin or a probiotic had little effect on the outcome of peptic or small intestinal incubations but tylosin appeared tc slow the degradation of glucosinolates in the presence of caecal contents.

Keywords

GlucosinolatesDegradationRapeseed meal
Type
Intestinal enzymes and the degradation of glucosinolates
Information
British Journal of Nutrition , Volume 72 , Issue 3 , September 1994 , pp. 455 - 466
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Daxenbichler, M. E., VanEtten, C. H. & Wolff, I. A. (1966). (S)- and (R)-l-cyano-2-hydroxy-3-butenefrom myrosinase-hydrolysis of epi-progoitrin and progoitrin. Biochemistry 5, 692–697.Google Scholar
Duncan, A. J. (1991). Toxic factors in crop plants. Proceedings of the Second Spring Conference, Edinburgh, pp. 1–20 [D'Mello, J. P. F. and Duffus, C. M., editors]. Edinburgh: Scottish Agricultural Colleges.Google Scholar
Fenwick, G. R., Spinks, E. A., Wilson, A. P., Heaney, R. K. & Legoy, M. A. (1986). Etrect of processing on the antinutritional content of rapeseed. Journal of the Science of Food and Agriculture 37, 731–741.CrossRefGoogle Scholar
Hill, H. (1969). The flow of food through the monogastric animal, including the mechanism of the whole gut. In Handbuch der Tierernahrung, p. 172 [Lenkeit, w., K., Breiren and Crassmann, E., editors]. Hamburg: Parey Verlag.Google Scholar
Kawakishi, S., Namiki, M., Watanabe, H. & Muramatsu, K. (1967). Decomposition of sinalbin. 11. The decomposition products of sinalbin and their degradation pathways. Agricultural and Biological Chemistry 31, 823–830.Google Scholar
Keys, J. E. & Debarthe, J. V. (1974). Site and extent of carbohydrate, dry matter, energy and protein digestion and the rate of passage of grain diets in swine. Journal of Animal Science 39, 57–62.Google Scholar
Kidder, D. E., Manners, M. J. & McCrea, M. R. (1961). The passage of food through the alimentary tract of the piglet. Research in Veterinary Science 2, 227–234.Google Scholar
Maskell, I. (1990). Nutritional aspects of feeding rapeseed products to rats and pigs. PhD Thesis, University of Newcastle upon Tyne.Google Scholar
Minchinton, I., Sang, J., Burke, D. & Truscott, J. W. (1982). Separation of desulphoglucosinolates by reversed phase HPLC. Journal of Chromatography 247, 141–148.CrossRefGoogle Scholar
Pusztai, A. (1989). Antinutrients in rapeseeds. Nutrition Abstracts and Reviews Series B 59, 427–433.Google Scholar
Rowan, T. G., Lawrence, T. J. L. & Kershaw, S. J. (1991). Effects of dietary copper and a probiotic on glucosinolate concentrations in ileal digesta and in faeces of growing pigs given diets based on rapeseed meals. Animal Feed Science and Technology 35, 241–258.Google Scholar
Slominski, B. A., Campbell, L. D. & Stanger, N. E. (1987). Influence of cecectomy and dietary antibiotics on the fate of ingested glucosinolates in poultry. Canadian Journal of Animal Science 67, 11 17–1124.CrossRefGoogle Scholar
Slominski, B. A., Campbell, L. D. & Stanger, N. E. (1988). Extent of hydrolysis in the intestinal tract and potential absorption of intact glucosinolates in laying hens. Journal of the Science of Food and Agriculture 42, 305–314.Google Scholar