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Copper deficiency in chicks: effects of ascorbic acid on iron, copper, cytochrome oxidase activity, and aortic mucopolysaccharides

Published online by Cambridge University Press:  09 March 2007

C. E. Hunt ,
Joanne Landesman  and
P. M. Newberne
C. E. Hunt
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Joanne Landesman
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
P. M. Newberne
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
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Abstract

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1. Copper deficiency was induced in newly hatched chicks by feeding on a milk-based diet for 12 d; effects of supplementation with ascorbic acid were studied.

2. Cu deficiency alone resulted in 30% mortality from aortic rupture. This was associated with a 20% increase in total acid mucopolysaccharides in the aorta, manifested as an increase in chondroitin sulphate and a relative decrease in hyaluronic acid. Cytochrome oxidase activity of liver and heart was less than half that of the controls.

3. Supplementing the Cu-deficient diet with 0.5 % L-ascorbic acid increased mortality to 40%, raised total aortic acid mucopolysaccharides to a higher level, and increased liver iron by 36%.

4. Supplementing the control diet with ascorbic acid decreased liver Cu by 30% and significantly reduced total aortic acid mucopolysaccharides.

5. The enhancement of the Cu-deficiency effect by ascorbic acid probably results from interactions between ascorbic acid and absorption or metabolism of Cu; untoward effects of supplementing the control diet with ascorbic acid may be interpreted as manifestations of ascorbic acid toxicity per se.

Type
Research Article
Information
British Journal of Nutrition , Volume 24 , Issue 3 , September 1970 , pp. 607 - 614
Copyright
Copyright © The Nutrition Society 1970

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