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Conversion of α-linolenic acid to eicosapentaenoic, docosapentaenoic and docosahexaenoic acids in young women

Published online by Cambridge University Press:  09 March 2007

Graham C. Burdge  and
Stephen A. Wootton
Graham C. Burdge*
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton, UK
Stephen A. Wootton
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton, UK
*
*Corresponding author: Dr G. C. Burdge, fax +44 23 80794945, email [email protected]
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Abstract

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The extent to which women of reproductive age are able to convert the n−3 fatty acid α-linolenic acid (ALNA) to eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) was investigated in vivo by measuring the concentrations of labelled fatty acids in plasma for 21 d following the ingestion of [U−13C]ALNA (700 mg). [13C]ALNA excursion was greatest in cholesteryl ester (CE) (224 (SEM 70) μmol/l over 21 d) compared with triacylglycerol (9-fold), non-esterified fatty acids (37-fold) and phosphatidylcholine (PC, 7-fold). EPA excursion was similar in both PC (42 (SEM 8) μmol/l) and CE (42 (SEM 9) μmol/l) over 21 d. In contrast both [13C]DPA and [13C]DHA were detected predominately in PC (18 (SEM 4) and 27 (SEM 7) μmol/l over 21 d, respectively). Estimated net fractional ALNA inter-conversion was EPA 21 %, DPA 6 % and DHA 9 %. Approximately 22 % of administered [13C]ALNA was recovered as 13CO2 on breath over the first 24 h of the study. These results suggest differential partitioning of ALNA, EPA and DHA between plasma lipid classes, which may facilitate targeting of individual n−3 fatty acids to specific tissues. Comparison with previous studies suggests that women may possess a greater capacity for ALNA conversion than men. Such metabolic capacity may be important for meeting the demands of the fetus and neonate for DHA during pregnancy and lactation. Differences in DHA status between women both in the non-pregnant state and in pregnancy may reflect variations in metabolic capacity for DHA synthesis.

Keywords

Women-Linolenic acidStable isotopesDocosahexaenoic acidPlasma
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 4 , October 2002 , pp. 411 - 420
Copyright
Copyright © The Nutrition Society 2002

References

Burdge, GC, Hunt, AN & Postle, AD (1994) Mechanisms of hepatic phosphatidylcholine synthesis in adult rat: effects of pregnancy. Biochemical Journal 303, 941–947.CrossRefGoogle ScholarPubMed
Burdge, GC, Jones, AE, Wright, P, Ware, L & Wootton, SA (2001) α-Linolenic acid metabolism in adult men: evidence for synthesis of eicosapentaenoic and docosapentaenoic acids, but not docosahexaenoic acid. Proceedings of the Nutrition Society 60, 22A.Google Scholar
Burdge, GC & Postle, AD (1994) Hepatic phospholipid molecular species in the guinea pig. Adaptations to pregnancy. Lipids 29, 259–264.CrossRefGoogle ScholarPubMed
Burdge, GC, Wright, P, Jones, AE & Wootton, SA (2000) A method for separation of phosphatidylcholine, triacylglycerol, non-esterified fatty acids and cholesterol esters from plasma by solid phase extraction. British Journal of Nutrition 84, 781–787.CrossRefGoogle ScholarPubMed
Calder, PC (1999) Dietary fatty acids and the immune system. Lipids 34, S137–S140.CrossRefGoogle ScholarPubMed
Chambaz, J, Ravel, D, Manier, MC, Pepin, D, Mulliez, N & Bereziat, G (1985) Essential fatty acid interconversion in the human liver. Biology of the Neonate 47, 136–140.CrossRefGoogle Scholar
Cho, HP, Nakamura, M & Clarke, SD (1999 a) Cloning, expression and nutritional regulation of the mammalian Δ-6 desaturase. Journal of Biological Chemistry 274, 471–477.CrossRefGoogle ScholarPubMed
Cho, HP, Nakamura, M & Clarke, SD (1999 b) Cloning, expression and fatty acid regulation of the human Δ-5 desaturase. Journal of Biological Chemistry 274, 37335–37339.CrossRefGoogle ScholarPubMed
Clandinin, MT, Chappell, JE, Hein, T, Swyer, PR & Chance, GW (1981) Fatty acid utilization in perinatal de novo synthesis of tissues. Early Human Development 5, 355–366.CrossRefGoogle ScholarPubMed
Eden, S, Oscarsson, J, Jansson, JO & Svanborg, A (1987) The influence of gonadal steroids and the pituitary on the levels and composition of plasma phospholipids in the rat. Metabolism 36, 527–532.CrossRefGoogle ScholarPubMed
Emken, EA, Adolf, RO & Gully, RM (1994) Dietary linoleic acid influences desaturation and acylation of deuterium-labelled linoleic and linolenic acids in young adult males. Biochimica et Biophysica Acta 1213, 277–288.CrossRefGoogle Scholar
Folch, JL, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Gerster, H (1998) Can adults convert α-linolenic acid (18:3n−3) to eicosapentaenoic acid (20:5n−3) and docosahexaenoic acid (22:5n−3)? International Journal of Vitamin and Nutrition Research 68, 159–173.Google ScholarPubMed
Innis, SM (1991) Essential fatty acids in growth and development. Progress in Lipid Research 30, 39–103.CrossRefGoogle ScholarPubMed
Irving, CS, Wong, WW, Schulman, RJ, Smith, EO & Klein, PD (1983) [13C]Bicarbonate kinetics in humans: intra- vs. inter-individual variations. American Journal of Physiology 245, R190–R202.Google Scholar
Jones, AE, Murphy, JL, Stolinski, M & Wootton, SA (1998) The effect of age and gender on the metabolic disposal of 1-13C palmitic acid. European Journal of Clinical Nutrition 52, 22–28.CrossRefGoogle ScholarPubMed
Jones, AE, Stolinski, M, Smith, RD, Murphy, JL & Wootton, SA (1999) Effect of fatty acid chain length and saturation on the gastrointestinal handling and metabolic disposal of dietary fatty acids in women. British Journal of Nutrition 81, 37–43.CrossRefGoogle ScholarPubMed
Li, Z, Kaplan, ML & Hatchey, DL (2000???) Hepatic microsomal and peroxisomal docosahexaenoate biosynthesis during piglet development. Lipids 35, 1325–1333.CrossRefGoogle ScholarPubMed
Ministry of Agriculture, Fisheries and Food (1997) Dietary Intake of Iodine and Fatty Acids. Food Information Surveillance Sheet no. 127. London: Ministry of Agriculture, Fisheries and Food.Google Scholar
Neuringer, M, Connor, WE, Van Petten, C & Barsrad, L (1984) Dietary omega-3 fatty acid deficiency and visual loss in infant rhesus monkeys. Journal of Clinical Investigation 73, 272–276.CrossRefGoogle ScholarPubMed
Otto, SJ, Van Houwelingen, AC, Manninen, A, Godfrey, K, Lopez-Jaramillo, P & Hornstra, G (1997) Maternal and neonatal essential fatty acid status in phospholipids: an international comparative study. European Journal of Clinical Nutrition 51, 232–242.CrossRefGoogle ScholarPubMed
Ottosson, UB, Lagrelius, A, Rosing, U & von Schoultz, B (1984) Relative fatty acid composition of lecithin during postmenopausal replacement therapy – a comparison between ethynyl estradiol and estradiol valerate. Gynecology and Obstetric Investigation 18, 296–302.CrossRefGoogle ScholarPubMed
Postle, AD, Al, MDM, Burdge, GC & Hornstra, G (1995???) The composition of individual molecular species of plasma phosphatidylcholine in human pregnancy. Early Human Development 43, 47–58.CrossRefGoogle ScholarPubMed
Salem, N, Powlosky, R, Wegher, B & Hibbeln, J (1999) In vivo conversion of linoleic acid to arachidonic acid in human adults. Prostaglandins, Leukotrienes and Essential Fatty Acids 60, 407–410.CrossRefGoogle ScholarPubMed
Silfverstolpe, G, Johnson, P, Samsice, G, Svanborg, A & Gustafson, A (1981) Effects induced by two different estrogens on serum individual phospholipids and serum lecithin fatty acid composition. Hormone and Metabolic Research 13, 141–145.CrossRefGoogle ScholarPubMed
Sprecher, H (2000) Metabolism of highly unsaturated n−3 and n−6 fatty acids. Biochimica et Biophysica Acta 1486, 219–231.CrossRefGoogle Scholar
Su, HM, Bernardo, L, Mirmiran, M, Ma, XH, Corso, TN, Nathanielsz, PW & Brenna, JT (1999 a) Bioequivalence of dietary alpha-linolenic and docosahexaenoic acids as sources of docosahexaenoate accretion in brain and associated organs of neonatal baboons. Pediatric Research 45, 87–93.CrossRefGoogle ScholarPubMed
Su, HM, Bernardo, L, Mirmiran, M, Ma, XH, Nathanielsz, PW & Brenna, JT (1999 b) Dietary 18:3n−3 and 22:6n−3 as sources of 22:6n−3 accretion in neonatal baboon brain and associated organs. Lipids 34, Suppl., S347–S350.CrossRefGoogle ScholarPubMed
Toth, MJ, Gardner, AW, Arciero, PJ, Calles-Escandon, J & Poehlman, ET (1998) Gender differences in fat oxidation and sympathetic nervous system activity during submaximal exercise in older individuals. Clinical Science 95, 59–66.CrossRefGoogle ScholarPubMed
Vermunt, SHF, Mensink, RP, Simonis, AMG & Hornstra, G (2000) Effects of dietary α-linolenic acid on the conversion and oxidation of [13C]-α-linolenic acid. Lipids 35, 137–142.CrossRefGoogle ScholarPubMed
Voss, A, Reinhart, M, Sankarappa, S & Sprecher, H (1991) The metabolism of 7,10,13,16,19-docosapentaenoic acid to 4,7,10,13,16,19-docosahexaenoic acid in rat liver is independent of a 4-desaturase. Journal of Biological Chemistry 266, 19995–20000.CrossRefGoogle Scholar
Watkins, JB, Klein, PD, Schoeller, DA, Kirschner, BS, Park, R & Perman, JA (1982) Diagnosis and differentiation of fat malabsorption in children using [13C]-labelled lipids: trioctanoin, triolein and palmitic acid breath tests. Gastroenterology 82, 911–917.CrossRefGoogle ScholarPubMed