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Composition of bacteria harvested from the liquid and solid fractions of the rumen of sheep as influenced by feed intake

Published online by Cambridge University Press:  09 March 2007

C. A. Rodríguez ,
J. González ,
M. R. Alvir ,
J. L. Repetto ,
C. Centeno  and
F. Lamrani
C. A. Rodríguez*
Affiliation:
Departamento de Producción Animal, Universidad Politécnica de Madrid, 28040 Madrid, Spain
J. González
Affiliation:
Departamento de Producción Animal, Universidad Politécnica de Madrid, 28040 Madrid, Spain
M. R. Alvir
Affiliation:
Departamento de Producción Animal, Universidad Politécnica de Madrid, 28040 Madrid, Spain
J. L. Repetto
Affiliation:
Departamento de Producción Animal, Universidad Politécnica de Madrid, 28040 Madrid, Spain
C. Centeno
Affiliation:
Consejo Superior de Investigaciones Científicas, Instituto de Nutrición y Bromatología, 28040 Madrid, Spain
F. Lamrani
Affiliation:
Departamento de Producción Animal, Universidad Politécnica de Madrid, 28040 Madrid, Spain
*
*Corresponding author: Dr C. A. Rodríguez, fax +34 91 549 97 63, email [email protected]
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Abstract

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A study was conducted to determine the effect of the feed intake on the chemical composition of bacteria associated with the solid (solid-associated bacteria; SAB) and liquid (liquid-associated bacteria; LAB) fractions of rumen digesta, the digestive passage kinetics and their relationships. Whole rumen contents were sampled after a period of continuous infusion of 15NH3 from four ruminally-cannulated wethers provided successively with a hay–concentrate diet (2: 1 w/w on a DM basis) at two rates of feed intake: 40 and 80 g DM/kg body weight0·75. SAB had a higher content of organic matter and total lipids (P < 0·001) and a similar N content as compared with LAB. The concentration of purines and 15N was lower (P = 0·011 and P < 0·001 respectively) in SAB than LAB, whereas the opposite was observed for the concentration of amino acids (mg/g DM; P = 0·031). An increase in feed intake produced an increase in the N (P = 0·034) and purine (P = 0·066) concentrations in bacteria and a decrease (P = 0·033) in their amino acid concentrations. Significant increases of rumen outflow rates of liquid and particles were also observed with increased feed intake. Rates of rumen outflow showed positive and negative linear relationships (P < 0·001) with the purine: N ratio and the proportion of amino acid on total N of bacteria respectively. SAB contained significantly higher proportions of leucine, isoleucine, lysine and phenylalanine and lower proportions of alanine, methionine and valine than LAB. The increase in feed intake also induced significant changes in the amino acid profile of bacteria, increasing arginine and methionine and decreasing alanine and glycine proportions. Results show that the outflow rate of rumen contents is a major factor in determining the proportion of nucleic acids and protein in rumen bacteria and explains some of the differences observed between LAB and SAB.

Keywords

Rumen bacteriaChemical compositionFeed intake
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 3 , September 2000 , pp. 369 - 376
Copyright
Copyright © The Nutrition Society 2000

References

Association of Official Analytical Chemists (1984) Official Methods of Analysis, 14th ed. Arlington, VA: AOAC.Google Scholar
Balcells, J, Guada, JA, Peiró JM and Parker, DS (1992) Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography.Journal of Chromatography 575, 153–157.CrossRefGoogle ScholarPubMed
Bates, DB, Gillett, JA, Barao, SA and Bergen, WR (1985) The effect of specific grow rate and stage of growth on nucleic acid-protein values of pure cultures and mixed ruminal bacteria.Journal of Animal Science 61, 713–724.CrossRefGoogle Scholar
Bauchart, D, Legay-Carmier, F, Doreau, M and Jouany, JP (1986) Effects of the addition of non-protected fat in rations for milk cows over the concentration and chemical composition of rumen bacteria and protozoa.Reproduction Nutrition Développement 26, 309–310.CrossRefGoogle Scholar
Beckers, Y, Thewis, A, Maudoux, B and Francois, E (1995) Studies on the in situ nitrogen degradability corrected for bacterial contamination of concentrate feeds in steers.Journal of Animal Science 73, 220–227.CrossRefGoogle ScholarPubMed
Benchaar, C, Bayourthe, C, Vernay, M and Moncoulon, R (1995) Composition chimique des bactéries libres ou adhérentes au contenu du rumen et du duodénum chez la vache (Chemical composition of bacteria free or attached to rumen or duodenum content in cows).Annales de Zootechnie 44 (Suppl.), 139.CrossRefGoogle Scholar
Bremer, H & Dennis, PP (1987) Modulation of chemical composition and other parameters of the cell by growth rate. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, vol. 2, chapter 96 [Neidhardt, FC, Ingraham, JL, KB, Low, Magasanik, B, Schaechter, M and Umbarger, HE, editors]. Washington, DC: American Society for Microbiology.Google Scholar
Cecava, MJ, Merchen, NR, Gay, LC and Berger, LL (1990) Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency, and isolation techniques.Journal of Dairy Science 73, 2480–2488.CrossRefGoogle ScholarPubMed
Craig, WH, Broderick, GA and Bradford, RO (1987) Quantitation of micro-organisms associated with the particulate phase of ruminal digesta.Journal of Nutrition 117, 56–62.CrossRefGoogle Scholar
Craig, WM, Brown, DR, Broderick, GA and Ricker, DB (1987) Post-prandial compositional changes of fluid- and particle-associated ruminal micro-organisms.Journal of Animal Science 65, 1042–1048.CrossRefGoogle Scholar
Cummins, CS (1989) Bacterial cell wall structure. In Practical Handbook of Microbiology pp. 349–379 [O'Leary, MW, editor]. Boca Raton, FL: CRC Press Inc.Google Scholar
Dhanoa, MS, Siddons, RC, France, J and Gale, DL (1985) A multicompartmental model to describe marker excretion patterns in ruminant faeces.British Journal of Nutrition 53, 663–671.CrossRefGoogle ScholarPubMed
Dixon, RM, Nolan, JV and Milligan, LP (1982) Studies of the large intestine of sheep. 2 — Kinetics of liquid and solid phase markers in the caecum and proximal colon.British Journal of Nutrition 47, 301–309.CrossRefGoogle ScholarPubMed
Ellis, WC & Beever, KC (1984) Methods for binding rare earth to specific feed particles. In Techniques in Particle Size Analysis of Feed and Digesta in Ruminants, pp. 154–165 [Kennedy, CPM, editor]. Edmonton, Alberta: Canadian Society of Animal Science.Google Scholar
Ellis, W, Matis, JH and Lascano, C (1979) Quantitating ruminal turnover.Federation Proccedings 38, 2702–2706.Google ScholarPubMed
Ellis, Wylie MJ & Matis, JH (1988) Dietary-digestive interactions determining the feeding value of forages and roughages. In Feed Science, pp. 177–299 [Ørskov, ER, editor]. Amsterdam: Elsevier Science Publishers BV.Google Scholar
Firkins, JL, Berger, LL, Merchen, NR, Fahey, GC and Mulvaney, RL (1987) Ruminal nitrogen metabolism in steers as affected by feed intake and dietary urea concentration.Journal of Dairy Science 70, 2302–2311.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M and Sloane Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues.Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Grovum, WL and Williams, VJ (1973) Rate of passage of digesta in sheep. 4. Passage of marker through the alimentary tract and the biological relevance of rate-constants derived from the changes in concentration of marker in faeces.British Journal of Nutrition 30, 313–329.CrossRefGoogle ScholarPubMed
Harfoot, GG (1981) Lipid metabolism in the rumen. In Lipid Metabolism in Ruminant Animals, pp. 21–55 [Christie, WW, editor]. Oxford: Pergamon Press.CrossRefGoogle Scholar
Hespell, RB and Bryant, MP (1979) Efficiency of rumen microbial growth: influence of some theoretical and experimental factors on Y ATP.Journal of Animal Science 49, 1640–1659.CrossRefGoogle Scholar
Jones, BR, Pääbo S and Stein, S (1981) Amino acid analysis and enzymatic sequence determination of peptides by an improved o-phthaldialdehyde precolumn labelling procedure.Journal of Liquid Chromatography 4, 565–586.CrossRefGoogle Scholar
Komisarczuk, S, Durand, M, Beaumatin Ph and Hannequart, G (1987) Utilisation de l'azote 15 pour la mesure de la protéosynthése microbienne dans les phases solide et liquide d'un fermenteur semi-continu (Rusitec) (Use of 15N to measure the microbial protein synthesis in the solid and liquid phases of a semi-continuous fermenter (Rusitec)).Reproduction Nutrition Dévelopement 27, 261–262.CrossRefGoogle Scholar
Latham, MJ (1980) Adhesion of rumen bacteria to plant cell walls. In Microbial Adhesion to Surfaces, pp. 339–350 [Berkeley, RCW, Lynch, JM, Melliney, JRutter, RP and Vincent, B, editors]. Chichester: Ellis Howard.Google Scholar
Legay-Carmier, F and Bauchart, D (1989) Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soyabean oil.British Journal of Nutrition 61, 725–740.CrossRefGoogle Scholar
Martin, C, Williams, AG and Michalet-Doreau, B (1994) Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents.Journal of Animal Science 72, 2962–2968.CrossRefGoogle ScholarPubMed
Martin, C, Bernard, L and Michalet-Doreau, B (1996) Influence of sampling time and diet on amino acid composition of protozoal and bacterial fractions from bovine ruminal contents.Journal of Animal Science 74, 1157–1163.CrossRefGoogle ScholarPubMed
Merry, RJ and McAllan, AB (1983) A comparison of the chemical composition of mixed bacteria harvested from the liquid and solids fractions of rumen digesta.British Journal of Nutrition 50, 701–709.CrossRefGoogle ScholarPubMed
Olubobokun, JA and Craig, WM (1990) Quantity and characteristics of micro-organisms associated with ruminal fluid or particles.Journal of Animal Science 68, 3360–3370.CrossRefGoogle ScholarPubMed
Olubobokun, JA, Craig, WM and Pond, KR (1990) Effects of mastication and microbial contamination on ruminal in situ forage disappearance.Journal of Animal Science 68, 3371–3381.CrossRefGoogle ScholarPubMed
Pirt, SJ (1975) Principles of Microbe and Cell Cultivation. Oxford: Blackwell Science Publishers.Google Scholar
Poncet, C (1991) The outflow of particles from the reticulo-rumen. In Rumen Microbial Metabolism and Ruminant Digestion, pp. 297–322 [Jouany, JP, editor]. San Diego, CA: Academic Press.Google Scholar
Robertson, JB & Van Soest, PJ (1981) The detergent system of analysis and its application to human foods. In The Analysis of Dietary Fiber in Food, pp. 123–158 [James, WPT and Theander, O, editors]. New York, NY: Marcel Dekker.Google Scholar
Schaechter, M, Maaløe, O and Kjeldgaard, NO (1958) Dependency on medium and temperature of cell size and chemical composition during balanced growth of.Salmonella typhimuriumJournal of General Microbiology 19, 592–606.CrossRefGoogle Scholar
Uden, P, Colucci, PE and Van Soest, PJ (1980) Investigation of chromium, cerium and cobalt as markers in digesta. Rate of passage studies.Journal of the Science of Food and Agriculture 31, 625–632.CrossRefGoogle ScholarPubMed
Waghorn, GC and Reid, CSW (1977) Rumen motility in sheep and cattle as effected by feeds and feeding.Proceedings of the New Zealand Society of Animal Production 37, 176.Google Scholar
Williams, AG and Strachan, NH (1984) Polysaccharide degrading enzymes in microbial population from the liquid and solid fractions of bovine rumen digesta.Canadian Journal of Animal Science 64 (Suppl.), 58–59.CrossRefGoogle Scholar