Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-18T04:54:17.590Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparative 2H-labelled α-tocopherol biokinetics in plasma, lipoproteins, erythrocytes, platelets and lymphocytes in normolipidaemic males

Published online by Cambridge University Press:  08 March 2007

Yvonne M. Jeanes ,
Wendy L. Hall  and
John K. Lodge
Yvonne M. Jeanes
Affiliation:
Centre for Nutrition and Food Safety, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
Wendy L. Hall
Affiliation:
Centre for Nutrition and Food Safety, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
John K. Lodge*
Affiliation:
Centre for Nutrition and Food Safety, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
*
*Corresponding author: Dr John K. Lodge, fax +44 (0)1483 876 416, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The biokinetics of newly absorbed vitamin E in blood components was investigated in normolipidaemic males. Subjects (n 12) ingested encapsulated 150 mg 2H-labelled RRR-α-tocopheryl acetate with a standard meal. Blood was collected at 3, 6, 9, 12, 24 and 48 h post-ingestion. 2H-Labelled and pre-existing unlabelled α-tocopherol (α-T) was determined in plasma, lipoproteins, erythrocytes, platelets and lymphocytes by LC–MS. In all blood components, labelled α-T concentration significantly increased while unlabelled decreased following ingestion (P<0·0001). Significant differences in labelled α-T biokinetic parameters were found between lipoproteins. Time of maximum concentration was significantly lower in chylomicrons, while VLDL had a significantly greater maximum α-T concentration and area under the curve (AUC) (P<0·05). The largest variability occurred in chylomicron α-T transport. Erythrocyte labelled α-T concentrations increased gradually up to 24 h while α-T enrichment of platelets and lymphocytes was slower, plateauing at 48 h. Platelet enrichment with labelled α-T was biphasic, the initial peak coinciding with the labelled α-T peak in chylomicrons. Erythrocyte and HDL AUC were significantly correlated (P<0·005), as was platelet and HDL AUC (P<0·05). There was a lower turnover of pre-existing α-T in platelets and lymphocytes (maximum 25 % labelled α-T) compared to plasma and erythrocytes (maximum 45 % labelled α-T). These data indicate that different processes exist in the uptake and turnover of α-T by blood components and that chylomicron α-T transport is a major determinant of inter-individual variation in vitamin E response. This is important for the understanding of α-T transport and uptake into tissues.

Keywords

DeuteratedTocopherolPlasmaErythrocytesPlateletsLymphocytes
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 1 , July 2005 , pp. 92 - 99
Copyright
Copyright © The Nutrition Society 2005

References

Acuff, RV, Thedford, SS, Hidiroglou, NN, Papas, AM, Odom, TA Jr (1994) Relative bioavailability of RRR- and all-rac-alpha-tocopheryl acetate in humans: studies using deuterated compounds. Am J Clin Nutr 60, 397–402.CrossRefGoogle ScholarPubMed
Brigelius-Flohe, R & Traber, MG (1999) Vitamin E: function and metabolism. FASEB J 13, 1145–1155.CrossRefGoogle Scholar
Burton, GW & Traber, MG (1990) Vitamin E: antioxidant activity biokinetics and bioavailability. Annu Rev Nutr 10, 357–382.CrossRefGoogle ScholarPubMed
Burton, GW, Webb, A & Ingold, KU (1985) A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios. Lipids 20, 29–39.CrossRefGoogle ScholarPubMed
Cheeseman, KH, Holley, AE, Kelly, FJ, Wasil, M, Hughes, L & Burton, G (1995) Biokinetics in humans of RRR-α-tocopherol: the free phenol, acetate ester, and succinate ester forms of vitamin E. Free Radic Biol Med 19, 591–598.CrossRefGoogle ScholarPubMed
Doring, F, Rimbach, G & Lodge, JK (2004) In silico search for single nucleotide polymorphisms in genes important in vitamin E homeostasis. IUBMB Life 56, 615–620.CrossRefGoogle ScholarPubMed
Freedman, JE, Farhat, JH, Loscalzo, J & Keaney, JF (1996) α-Tocopherol inhibits aggregation of human platelets by a protein kinase C-dependent mechanism. Circulation 94, 2434–2440.CrossRefGoogle ScholarPubMed
Goti, D, Hammer, A, Galla, HJ, Malle, E & Sattler, W (2000) Uptake of lipoprotein-associated alpha-tocopherol by primary porcine brain capillary endothelial cells. J Neurochem 74, 1374–1383.CrossRefGoogle ScholarPubMed
Goti, D, Hrzenjak, A, Levak-Frank, S, Frank, S, van der Westhuyzen, DR, Malle, E & Sattler, W (2001) Scavenger receptor class B, type I is expressed in porcine brain capillary endothelial cells and contributes to selective uptake of HDL-associated vitamin E. J Neurochem 76, 498–508.Google Scholar
Goulinet, S & Chapman, MJ (1997) Plasma LDL and HDL subspecies are heterogenous in particle content of tocopherols and oxygenated and hydrocarbon carotenoids. Relevance to oxidative resistance and atherogenesis. Arterioscler Thromb Vasc Biol 17, 786–796.CrossRefGoogle ScholarPubMed
Hall, WL, Jeanes, YM & Lodge, JK (2005) Hyperlipidemic subjects have reduced uptake of newly absorbed vitamin E into their plasma lipoproteins, erythrocytes, platelets, and lymphocytes, as studied by deuterium-labeled {alpha}-tocopherol biokinetics. J Nutr 135, 58–63.CrossRefGoogle ScholarPubMed
Hall, WL, Jeanes, YM, Pugh, J & Lodge, JK (2003) Development of a liquid chromatographic time-of-flight mass spectrometric method for the determination of unlabelled and deuterium-labelled alpha-tocopherol in blood components. Rapid Commun Mass Spectrom 17, 2797–2803.CrossRefGoogle ScholarPubMed
Havel, RJ, Eder, HA & Bragdon, JH (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34, 1345–1353.CrossRefGoogle ScholarPubMed
Jeanes, YM, Hall, WL, Proteggente, AR & Lodge, JK (2004) Cigarette smokers have decreased lymphocyte and platelet α-tocopherol levels and increased excretion of the γ-tocopherol metabolite γ-carboxyethyl-hydroxychroman (γ-CEHC). Free Radic Res 38, 861–868.CrossRefGoogle ScholarPubMed
Kaempf, DE, Miki, M, Ogihara, T, Okamoto, R, Konishi, K & Mino, M (1994) Assessment of vitamin E nutritional status in neonates, infants and children – on the basis of alpha-tocopherol levels in blood components and buccal mucosal cells. Int J Vitam Nutr Res 64, 185–191.Google ScholarPubMed
Kayden, HJ & Bjornson, L (1972) The dynamics of vitamin E transport in the human erythrocyte. Ann N Y Acad Sci 203, 127–140.CrossRefGoogle ScholarPubMed
Kayden, HJ & Traber, MG (1993) Absorption, lipoprotein transport, and regulation of plasma concentrations of vitamin E in humans. J Lipid Res 34, 343–358.Google ScholarPubMed
Kitabchi, AE & Wimalasena, J (1982) Specific binding sites for d -alpha-tocopherol on human erythrocytes. Biochim Biophys Acta 684, 200–206.CrossRefGoogle ScholarPubMed
Kolleck, I, Schlame, M, Fechner, H, Looman, AC, Wissel, H & Rustow, B (1999) HDL is the major source of vitamin E for type II pneumocytes. Free Radic Biol Med 27, 882–890.CrossRefGoogle ScholarPubMed
Kostner, GM, Oettl, K, Jauhiainen, M, Ehnholm, C, Esterbauer, H & Dieplinger, H (1995) Human plasma phospholipid transfer protein accelerates exchange/transfer of alpha-tocopherol between lipoproteins and cells. Biochem J 305, Pt 2 659–667.CrossRefGoogle ScholarPubMed
Lodge, JK, Hall, WL, Jeanes, YM & Proteggente, AR (2004) Physiological factors influencing vitamin E biokinetics. Ann N Y Acad Sci 1031, 60–73.CrossRefGoogle ScholarPubMed
Mardones, P & Rigotti, A (2004) Cellular mechanisms of vitamin E uptake: relevance in alpha-tocopherol metabolism and potential implications for disease. J Nutr Biochem 15, 252–260.CrossRefGoogle ScholarPubMed
Mardones, P, Strobel, P, Miranda, S, Leighton, F, Quinones, V, Amigo, L, Rozowski, J, Krieger, M & Rigotti, A (2002) Alpha-tocopherol metabolism is abnormal in scavenger receptor class B type I (SR-BI)-deficient mice. J Nutr 132, 443–449.CrossRefGoogle Scholar
Perugini, C, Bagnati, M, Cau, C, Bordone, R, Paffoni, P, Re, R, Zoppis, E, Albano, E & Bellomo, G (2000) Distribution of lipid-soluble antioxidants in lipoproteins from healthy subjects. II. Effects of in vivo supplementation with alpha-tocopherol. Pharmacol Res 41, 65–72.CrossRefGoogle ScholarPubMed
Relou, IA, Hackeng, CM, Akkerman, JW & Malle, E (2003) Low-density lipoprotein and its effect on human blood platelets. Cell Mol Life Sci 60, 961–971.CrossRefGoogle ScholarPubMed
Roxborough, HE, Burton, GW & Kelly, FJ (2000) Inter- and intra-individual variation in plasma and red blood cell vitamin E after supplementation. Free Radic Res 33, 437–445.CrossRefGoogle ScholarPubMed
Roy, RM, Petrella, M & Ross, WM (1991) Modification of mitogen-induced proliferation of murine splenic lymphocytes by in vitro tocopherol. Immunopharmacol Immunotoxicol 13, 531–550.CrossRefGoogle ScholarPubMed
Traber, MG, Cohn, W & Muller, DPR (1993) Absorption, transport and distribution to tissues Vitamin E in Health and Disease, pp. 35–52 [Packer, L and Fuchs, J, editors]. New York: Marcel Dekker.Google Scholar
Traber, MG, Ingold, KU, Burton, GW & Kayden, HJ (1988) Absorption and transport of deuterium-substituted 2 R ,4’ R ,8’ R -α-tocopherol in human lipoproteins. Lipids 23, 791–797.CrossRefGoogle ScholarPubMed
Traber, MG & Kayden, HJ (1984) Vitamin E is delivered to cells via the high affinity receptor for low density lipoprotein. Am J Clin Nutr 40, 747–751.CrossRefGoogle ScholarPubMed
Traber, MG & Kayden, HJ (1989) α-Tocopherol as compared with γ-tocopherol is preferentially secreted in human lipoproteins. Ann N Y Acad Sci 570, 95–108.Google ScholarPubMed
Traber, MG, Lane, JC, Lagmay, N & Kayden, HJ (1992) Studies on the transfer of tocopherol between lipoproteins. Lipids 27, 657–663.CrossRefGoogle ScholarPubMed
Traber, MG, Olivecrona, T & Kayden, HJ (1985) Bovine milk lipoprotein lipase transfers tocopherol to human fibroblasts during triglyceride hydrolysis in vitro. J Clin Invest 75, 1729–1734.CrossRefGoogle ScholarPubMed
Traber, MG, Rader, D, Acuff, RV, Ramakrishnan, R, Brewer, HB & Kayden, HJ (1998) Vitamin E dose–response studies in humans with use of deuterated RRR-alpha-tocopherol. Am J Clin Nutr 68, 847–853.CrossRefGoogle ScholarPubMed
Traber, MG, Ramakrishnan, R & Kayden, KJ (1994) Human plasma vitamin E kinetics demonstrate rapid recycling of plasma RRR -α-tocopherol. Proc Natl Acad Sci U S A 91, 10005–10008.CrossRefGoogle ScholarPubMed
Weintraub, MS, Eisenberg, S & Breslow, JL (1987) Different patterns of postprandial lipoprotein metabolism in normal, type IIa, type III, and type IV hyperlipoproteinemic individuals. Effects of treatment with cholestyramine and gemfibrozil. J Clin Invest 79, 1110–1119.CrossRefGoogle ScholarPubMed