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Circulating triacylglycerol and apoE levels in response to EPA and docosahexaenoic acid supplementation in adult human subjects

Published online by Cambridge University Press:  09 March 2007

Richard Buckley ,
Bethan Shewring ,
Rufus Turner ,
Parveen Yaqoob  and
Anne M. Minihane
Richard Buckley
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, UK
Bethan Shewring
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, UK
Rufus Turner
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, UK
Parveen Yaqoob
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, UK
Anne M. Minihane*
Affiliation:
Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, UK
*
*Corresponding author: fax +44 118 9310080, Email [email protected]
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Abstract

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High doses of n–3 PUFA found in fish oils can reduce the circulating concentration of triacylglycerol (TG), which may contribute to the positive impact of these fatty acids on the risk of CVD. The present study aimed to establish the differential impact of EPA and docosahexaenoic (DHA) on plasma lipids and apo in adults. Forty-two normolipidaemic adult subjects completed a double-blind placebo controlled parallel study, receiving an EPA-rich oil (4·8 g EPA/d), DHA-rich oil (4·9 g DHA/d) or olive oil as control, for a period of 4 weeks. No effects of treatment on total cholesterol, LDL-cholesterol or HDL-cholesterol were evident. There was a significant 22 % reduction in TG level relative to the control value following the DHA treatment (P=0·032), with the 15 % decrease in the EPA group failing to reach significance (P=0·258). There were no significant inter-group differences in response to treatment for plasma apoA1, -C3 or -E levels, although a significant 15 % within-group increase in apoE was evident in the EPA (P=0·006) and DHA (P=0·003) groups. In addition, a within-group decrease in the apoA1:HDL-cholesterol ratio was observed in the DHA group, suggesting a positive impact of DHA on HDL particle size. The DHA intervention resulted in a significant increase in the proportion of EPA P=0·000 and DHA P=0·000 in plasma phospholipids, whilst significant increases in EPA P=0·000 and docosapentaenoic acid P=0·002, but not DHA P=0·193, were evident following EPA supplementation (P>0·05). Our present results indicate that DHA may be more efficacious than EPA in improving the plasma lipid profile.

Keywords

Eicosapentaenoic acidDocosahexaenoic acidFish oilsTriacylglycerolapolipoprotein E
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 477 - 483
Copyright
Copyright © The Nutrition Society 2004

References

Ågren, JJ, Hänninen, O, Julkunen, A, Fogelholm, L, Vidgren, H, Schwab, U, Pynnönen, O & Uusitupa, MFish diet, fish oil and docosahexaenoic acid rich oil lower fasting and postprandial plasma lipid levels. Eur J Clin Nutr (1996) 50, 765–771.Google ScholarPubMed
Alagona, C, Soro, A, Ylitalo, K, Salonen, JT & Taskinen, MRA low high density lipoprotein (HDL) level is associated with carotid artery intima–media thickness in asymptomatic members of low HDL families. Atherosclerosis (2002) 165, 309–316.CrossRefGoogle ScholarPubMed
Austin, MA, Breslow, JL, Hennekens, CH, Buring, JE, Willett, WC & Krauss, RMLow density lipoprotein subclass patterns and risk of myocardial infarction. J Am Med Assoc (1988) 260, 917–921.CrossRefGoogle ScholarPubMed
larke, SDPolyunsaturated fatty acid regulation of gene transcription: a mechanism to improve energy balance and insulin resistance. Br J Nutr (2000) 83, S59–S66.Google Scholar
Davidson, MH, Maki, KC, Kalkowski, JA, Schaefer, EJ, Torri, SA & Drennan, KBEffects of docosahexaenoic acid on serum lipoproteins in patients with combined hyperlipidaemia: a randomized, double-blind placebo-controlled trial. J Am Coll Nutr (1997) 16, 236–243.CrossRefGoogle Scholar
Folch, J, Lees, M & Sloane Stanley, GHA simple method for the isolation and purification of total lipides from animal studies. J Biol Chem (1957) 226, 497–509.CrossRefGoogle Scholar
Friedewald, WT & Levy, RIEstimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem (1972) 18, 499–502.CrossRefGoogle ScholarPubMed
Gianturco, SH & Bradley, WAPathophysiology of triglyceride-rich lipoproteins in atherothrombosis: cellular aspects. Clin Cardiol (1999) 22, II7–II14.CrossRefGoogle ScholarPubMed
Frøyland, L, Vaagenes, H, Asiedu, DK, Garras, A, Lie, O & Berge, RKChronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs. Lipids (1996) 31, 169–178.CrossRefGoogle ScholarPubMed
Griffin, BA, Freeman, DJ, Tait, GW, Thomson, J, Caslake, MJ, Packard, CJ & Shepherd, JRole of plasma triglyceride in the regulation of plasma low density lipoprotein (LDL) subfractions: relative contribution of small, dense LDL to coronary heart disease risk. Atherosclerosis (1994) 106, 241–253.CrossRefGoogle ScholarPubMed
Grimsgaard, S, Bonaa, KH, Hansen, JB & Nordoy, AHighly purified eicosapentaenoic acid and docosahexaenoic acid in humans have similar triacylglycerol-lowering effects but divergent effects on serum fatty acids. Am J Clin Nutr (1997) 66, 649–659.CrossRefGoogle ScholarPubMed
Hamilton, RL, Wong, JS, Guo, LS, Krisans, S & Havel, RJApolipoprotein E localization in rat hepatocytes by immunogold labelling of cryothin sections. J Lipid Res (1990) 31, 1589–1603.CrossRefGoogle ScholarPubMed
Hansen, JB, Grimsgaard, S, Nilsen, H, Nordoy, A & Bonaa, KHEffect of highly purified eicosapentaenoic acid and docosahexaenoic acid on fatty acid absorption, incorporation into serum phospholipids and postprandial triglyceridemia. Lipids (1998) 33, 31–38.CrossRefGoogle ScholarPubMed
Harris, WSn -3 Fatty acids and serum lipoproteins: human studies. Am J Clin Nutr (1997) 65, 1645S–1654S.CrossRefGoogle ScholarPubMed
Hodis, HNTriglyceride rich lipoprotein remnant particles and risk of atherosclerosis. Circulation (1999) 99, 2852–2854.CrossRefGoogle Scholar
Hokanson, JE & Austin, MAPlasma triglyceride is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol; a meta analysis of population based studies. J Cardiovasc Risk (1996) 3, 213–219.CrossRefGoogle Scholar
Huang, Y, Ji, Z-S, Brecht, WJ, Rall, SC, Taylor, JM & Mahley, RWOverexpression of apolipoprotein E3 in transgenic rabbits causes combined hyperlipidemia by stimulating hepatic VLDL production and impairing VLDL lipolysis. Arterioscler Thromb Vasc Biol (1999) 19, 2952–2959.CrossRefGoogle ScholarPubMed
Huang, Y, Liu, XQ, Rall, SC, Jr Taylor, JM, von Eckardstein, A, Assmann, G & Mahley, RWOverexpression and accumulation of apolipoprotein E as a cause of hypertriglyceridaemia. J Biol Chem (1998) 273, 26388–26393.CrossRefGoogle Scholar
Jong, MC, Hofker, MH & Havekes, LMRole of apoCs in lipoprotein metabolism. Arterioscler Thromb Vasc Biol (1999) 19, 472–498.CrossRefGoogle ScholarPubMed
Jump, DB & Clarke, SDRegulation of gene expression by dietary fat. Annu Rev Nutr (1999) 19, 63–90.CrossRefGoogle ScholarPubMed
Karpe, FPostprandial lipid metabolism in relation to coronary heart disease. Proc Nutr Soc (1997) 56, 671–678.CrossRefGoogle ScholarPubMed
Khan, S, Minihane, AM, Talmud, PJ, Wright, JW, Murphy, MC, Williams, CM & Griffin, BADietary long chain n -3 PUFA increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype. J Lipid Res (2002) 43, 979–985.CrossRefGoogle ScholarPubMed
Krauss, RMDense low-density lipoproteins and coronary artery disease. Am J Cardiol (1995) 75, 53B–57B.CrossRefGoogle ScholarPubMed
Krul, ES, Tikkanen, MJ, Cole, TG, Davie, JM & Schonfeld, GRoles of apolipoproteins B and E in the cellular binding of very low-density lipoproteins. J Clin Invest (1985) 75, 361–369.CrossRefGoogle Scholar
Leigh-Firbank, EC, Minihane, AM, Leake, DS, Wright, JW, Murphy, MC, Briffin, BA & Williams, CMEicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses. Br J Nutr (2002) 87, 435–445.CrossRefGoogle ScholarPubMed
Madsen, L, Rustan, AC, Vaagenes, H, Berge, K, Dyrøy, E & Berge, RKEicosapentaenoic and docosahexaenoic acid affect mitochondrial and peroxisomal fatty acid oxidation in relation to substrate preference. Lipids (1999) 34, 951–963.CrossRefGoogle ScholarPubMed
Mahley, RWapolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science (1988) 240, 622–630.CrossRefGoogle ScholarPubMed
Mahley, RW & Ji, Z-SRemnant lipoprotein metabolism: key pathways involving cell-surface heparin sulphate proteoglycans and apolipoprotein E. J Lipid Res (1999) 40, 1–16.CrossRefGoogle Scholar
Mamo, JCL, Proctor, SD & Smith, DRetention of chylomicron remnants by arterial tissue: importance of an efficient clearance mechanism from plasma. Atherosclerosis (1998) 141, S63–S69.CrossRefGoogle ScholarPubMed
Minihane, AM, Khan, S, Leigh-Firbank, EC, Talmud, PJ, Wright, JW, Murphy, MC, Griffin, BA & Williams, CMapoE polymorphism and fish oil supplementation in subjects with an atherogenic lipoprotein phenotype (ALP). Arterioscler Thromb Vasc Biol (2000) 20, 1990–1997.CrossRefGoogle Scholar
Mori, TA, Burke, V, Puddey, IB, Watts, GF, O'Neal, DN, Best, JD & Beilin, LJPurified eicosapentaenoic and docosapentaenoic acids have differential effects on serum lipids and lipoproteins, LDL particle size, glucose, and insulin in mildly hyperlipidemic men. Am J Clin Nutr (2000) 71, 1085–1094.CrossRefGoogle Scholar
Nelson, GJ, Schmidt, PC, Bartolini, GL, Kelley, DS & Kyle, DThe effect of dietary docosahexaenoic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids (1997) 32, 1137–1146.CrossRefGoogle ScholarPubMed
Nordøy, A, Hansen, J-B, Brox, J & Svensson, BEffects of atorvastatin and ω-3 fatty acids on LDL subfractions and postprandial hyperlipidemia in patients with combined hyperlipidemia. Nutr Metab Cardiovasc Dis (2001) 11, 7–16.Google Scholar
Park, Y & Harris, WSOmega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance. J Lipid Res (2003) 44, 455–463.CrossRefGoogle ScholarPubMed
Price, PT, Nelson, CM & Clarke, SDOmega-3 polyunsaturated fatty acid and regulation of gene expression. Curr Opin Lipidol (2000) 11, 3–7.CrossRefGoogle ScholarPubMed
Rambjør, GS, Wålen, AI, Windsor, SL & Harris, WSEicosapentaenoic acid is primarily responsible for hypotriglyceridemic effect of fish oil in humans. Lipids (1996) 31, S45–S49.CrossRefGoogle ScholarPubMed
Salah, D, Bohnet, K, Gueguen, R, Siest, G & Visvikis, SCombined effects of lipoprotein lipase and apolipoprotein E polymorphisms on lipid and lipoprotein levels in the Stanislas cohort. J Lipid Res (1997) 38, 904–912.CrossRefGoogle ScholarPubMed
Tomiyasu, K, Walsh, BW, Ikewaki, K, Judge, H & Sacks, FMDifferential metabolism of human Veldt according to content of apoE and apoC-III. Arterioscler Thromb Vasc Biol (2001) 21, 1494–1500.CrossRefGoogle Scholar
Willumsen, N, Vaagenes, H, Lie, O, Rustan, AC & Berge, RKEicosapentaenoic acid but not docosahexaenoic acid increases mitochondrial fatty acid oxidation and upregulates 2,4-dienoyl-CoA reductase gene expression in rats. Lipids (1996) 31, 579–592.CrossRefGoogle Scholar
Xu, J, Nakamura, MT, Cho, HP & Clarke, SDSterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids. J Biol Chem (1999) 274, 840–848.Google ScholarPubMed
Zhang, ZJ, Wilcox, HG, Elam, MB, Castellani, LW & Heimberg, MMetabolism of n -3 polyunsaturated fatty acids by the isolated perfused rat liver. Lipids (1991) 26, 504–511.CrossRefGoogle Scholar