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Changes in glutathione status and 3,5,3'-triiodothyronine action in livers of rats given cysteine-deficient diets

Published online by Cambridge University Press:  09 March 2007

H. Garcin ,
C. Suberville ,
P. Higueret  and
D. Higueret
H. Garcin
Affiliation:
Laboratoire Physiologie de la Nutrition, Département Alimentation et Nutrition, Université de Bordeaux I, Avenue des Facultés, 33405 Talence-Cédex, France
C. Suberville
Affiliation:
Laboratoire Physiologie de la Nutrition, Département Alimentation et Nutrition, Université de Bordeaux I, Avenue des Facultés, 33405 Talence-Cédex, France
P. Higueret
Affiliation:
Laboratoire Physiologie de la Nutrition, Département Alimentation et Nutrition, Université de Bordeaux I, Avenue des Facultés, 33405 Talence-Cédex, France
D. Higueret
Affiliation:
Laboratoire Biochimie Médicale, Hôpital Pellegrin, Place Raba Léon, 33000 Bordeaux, France
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Abstract

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1. For a period of 32 d young rats were given a diet containing (g/kg) 220 casein, 120 casein +1.93 L-cysteine (Cys), or 120 casein.

2. The formation of 3,5,3'-triiodothyronine (T3)-nuclear protein complexes was reduced in rats fed on the Cys-deficient diet.

3. Scatchard analysis showed that decreased formation of T3 -nuclear protein complexes was due to a decreased affinity of T3 receptors; this decrease was induced, at least in part, by a reduced glutathione content.

4. In rats fed on the Cys-deficient diet there was an expected decrease in growth but an unexpected increase in the activities of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40). It is suggested that this increase is related to an increased oxidized glutathione: reduced glutathione ratio.

Type
Research Article
Information
British Journal of Nutrition , Volume 61 , Issue 2 , March 1989 , pp. 301 - 307
Copyright
Copyright © The Nutrition Society 1989

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