According to a growing body of literature, early feeding experiences can have important impact on later food and taste acceptance(Reference Beauchamp and Mennella1–Reference Nicklaus4), which is one of the major determinants of food consumption in children(Reference Nicklaus, Issanchou, Frewer and van Trijp5). Among early experiences, those related to milk feeding, whether it is breast-feeding or formula feeding, because they constitute the very first taste experience, could particularly have an impact on later food acceptance(Reference Mennella6).
One of the most illustrative examples of this phenomenon is the impact of feeding a hydrolysed protein formula, which is sour- and bitter-tasting(Reference Arai and Charalambous7, Reference Pedrosa, Pascual and Larco8), prescribed in the case of allergy to cows' milk protein, on later preferences. Infants exposed to such a formula during their first 7 months readily accepted it at 7·5 months, whereas it was rejected by non-exposed infants(Reference Mennella, Griffin and Beauchamp9). Moreover, a longer exposure to this formula was associated with its higher acceptance at 7·5 months(Reference Mennella, Griffin and Beauchamp9). Such exposure has also a long-term impact: a sour apple juice, but not a bitter apple juice, was more accepted by 4–5-year-old children previously exposed to this hydrolysed protein formula than by non-exposed children(Reference Mennella and Beauchamp10). This effect was no longer observed at 6–7 years(Reference Liem and Mennella11).
Nowadays, the beneficial impact of breast-feeding on different functions and, in particular, on the infant's early immunity has become obvious and is recognised through international nutritional policies, which recommend to exclusively breast-feed up until the age of 6 months(12, 13). Beyond these metabolic benefits of breast-feeding, and since early feeding experiences are likely to have an impact on later taste and food preferences, it is also important to understand better the sensory impact of breast-feeding(Reference Mennella6). Compared with exposure to formula, exposure to maternal milk may result in sensory difference in terms of aroma and taste. Concerning aroma, some volatile compounds from the foods ingested by the mother are likely to be transmitted into her milk(Reference Mennella and Beauchamp14, Reference Hausner, Bredie and Mølgaard15). Thus, breast milk may bear a distinct flavour component which is likely to have an impact on infant behaviour at the age of complementary feeding (about 6 months), as has been shown in several studies(Reference Mennella6, Reference Mennella, Jagnow and Beauchamp16, Reference Hausner, Nicklaus and Issanchou17). Concerning taste, breast milk contains some compounds which bear a taste, such as lactose (sweet taste), glutamate (umami taste), Na (salty taste) and urea (bitter taste)(Reference Jensen18). Their concentration in breast milk may differ from that in formula milks: the concentration of glutamate is 14-fold higher, but the concentration of Na is 2- to 4-fold lower(Reference Agostoni, Carratu and Boniglia19–Reference Alles, Scholtens and Bindels21). The impact of breast-feeding on later taste acceptance has been rarely assessed. A recent study indicated that breast-fed infants did not differ from infants fed a milk-based formula in their intake of cereals prepared with sweet, salty, sour, bitter and umami solutions, but they displayed more positive facial responses to umami-based cereals(Reference Mennella, Forestell and Morgan22). This may relate to a higher exposure to glutamate in breast milk compared with formula milks. Another study has revealed that the longer 16- to 25-week-old infants were breast-fed, the less they accepted their first salted cereals over plain cereals(Reference Harris, Thomas and Booth23). This was interpreted in relation to the low Na content of breast milk. The present study aimed at examining the impact of exclusive breast-feeding duration (DEB) on the acceptance of sweet, salty, sour, bitter and umami taste solutions at 6 and 12 months. Since breast milk has higher glutamate content than formula milks, we hypothesised that the longer an infant was breast-fed, the more s/he would be exposed to umami taste and thus the more s/he would accept umami taste. Similarly, because formula milk can contain up to twice as much Na as breast milk, breast-feeding could result in a diet lower in Na(Reference Mosser24), thus we hypothesised that the longer an infant was breast-fed, the less s/he would accept salty taste. We did not expect to observe an effect of DEB on the acceptance of sweet, sour and bitter tastes.
Subjects
The present study was part of the OPALINE programme (Observatory of Food Preferences in Infants and Children) aimed at understanding the formation of food preferences from birth until the age of 2 years. Participating mothers were recruited before the last trimester of pregnancy with the help of doctors, paediatricians, midwives, pharmacists and day-care centre staff. To be included in the study, parents had to have reached legal majority and infants had to be in good health at birth. Data from 137 infants were considered but infants fed a mixed formula and breast milk diet from birth on (n 10) were excluded from the present study because of non-exclusivity of breast milk feeding. Infants fed hydrolysed protein formula (n 5) were also excluded because this experience is associated with a very specific taste acceptance profile(Reference Mennella, Forestell and Morgan22). Here data are reported for 122 infants (sixty-two males), with a birth weight of 3·31 (sd 0·51) kg and a length at birth of 50·0 (sd 2·4) cm. The accompanying parent was usually the mother. The present study was conducted according to the guidelines laid down in the Declaration of Helsinki and all procedures involving parents and infants were approved by the CPP (Comité de Protection des Personnes) Est I Bourgogne. Written informed consent was obtained from both parents for all infants. Mother inclusion started in June 2005 and taste acceptance sessions were performed between July 2006 and September 2009.
Methods
Infant feeding history including breast-feeding history
From the birth of their infant on, the mothers were asked each month over the first year to fill in a 7 d food diary aimed at describing accurately the infant milk and solid feeding experience. In particular, they specified whether they exclusively breast-fed their infant (i.e. with no other milk and no other food) and, if so, the date when they stopped to do so. They also specified which type of formula milk their infant was fed over the first year, and when they started to introduce complementary foods. The DEB was calculated as the difference between the delivery date and the date when any food other than breast milk was introduced into the infant's diet. The age at the beginning of complementary feeding was calculated as the difference between the delivery date and the date when any food other than milk (breast or formula) was introduced.
Taste acceptance procedure
The taste acceptance procedure, adapted from previously proposed methods(Reference Beauchamp and Moran25, Reference Beauchamp, Cowart and Moran26), has been fully described elsewhere(Reference Schwartz, Issanchou and Nicklaus27). Infant taste acceptance was assessed at the corrected ages of 6 and 12 months (i.e. age considering the calculated delivery date, not the actual delivery date).
For each taste, a solution was prepared using mineral water (Evian®) and food-pharmacological-grade compounds (Jerafrance). The compounds chosen to represent each taste could have been encountered by infants in amniotic fluid or in their diet either in breast/formula milk or in solid food(Reference Jensen18, Reference Agostoni, Carratu and Boniglia28–Reference Muller, Dommergues and Bussières30) (Table 1). The concentrations were determined to be above adult detection thresholds, since infant detection thresholds might be in the same range as those of adults(Reference Cowart31), and to generate moderate intensities in order to avoid stereotypical reactions observed in some studies using high concentrations(Reference Steiner32). Sensory tests conducted with an adult panel (results not reported) confirmed that these supra-threshold concentrations generated perceptions of moderate intensities. At each age, infants participated in two videotaped sessions at approximately the same time of the day. Parents were asked not to feed their infant during the hour before the test session. For each taste, a fixed sequence of four bottles ‘water–tastant–tastant–water’ was presented to the infant by the experimenter. Within a sequence, each bottle was presented for 45 s with a 15 s pause between the bottles. Between two sequences, for all infants, a pause of at least 1 min was allowed. The five sequences corresponding to the five tastes were presented in a double-blind balanced order, over the two sessions. The mother and the experimenter wore a mask during all sequences to prevent any influence from their facial expressions(Reference Mennella, Forestell and Morgan22). To determine ingestion, bottles were weighed before and after consumption. Analysis was restricted to infants who met the ingestion criteria, i.e. who consumed at least 1·0 g from two bottles over a sequence. The ingestion ratio (IR) of a taste was defined as the ingested volume of this taste solution relative to the sum of the ingested volumes of this taste solution and of water. This IR varies by definition between 0 and 1. It can be interpreted as the following: 0·5 indicates indifference to the taste solution; ratio above 0·5 indicates a preference for the taste solution over water; ratio below 0·5 indicates a rejection of the taste solution over water.
Table 1 Compound and concentration used for the preparation of each taste solution
Statistical analyses
Statistical analyses were carried out using R version R2.8.0 (R Foundation for Statistical Computing; http://www.R-project.org). For infants who were not exclusively breast-fed at birth, the DEB was set at 0. For the analysis at 6 months, the DEB was truncated to 183 d (6 months × 30·5 d). For each age and each taste, a Student's t test (t df = n− 1) was used to assess whether the IR was different from 0·5 and thus whether the taste was preferred or rejected over water. A paired Student's t test (t df = n− 1) was also computed to evaluate whether taste acceptance differed between the ages of 6 and 12 months. Moreover, for each age and each taste, a Kendall correlation (τdf = n− 2) was calculated between the DEB and the IR. The significance criterion was set at 0·05. Results are expressed as means and standard deviations.
For each age and each taste, ANCOVA models were performed to evaluate the effect on taste acceptance (IR) of DEB and of the following covariates: sex; the difference between the expected and real birth date; duration since the introduction of complementary feeding. The results of these analyses were not reported since none of the covariates had a significant effect (P>0·05).
Results
Subjects' characteristics
The mothers were 31·3 (sd 4·1) years old on average and their average BMI was 22·2 (sd 3·4) kg/m2. Of the subjects, half were primiparous (50 %). A minority of the mothers gave birth by caesarean section (17 %).
Breast-feeding and solid feeding practices
The present analysis was focused on infants who either received no breast-feeding from birth on (13 %) or exclusive breast-feeding (87 %). The DEB was widely distributed: first quartile, 38 d; median, 103 d; third quartile, 154 d. Of the mothers who participated in the study, twenty (16 %) completed 6 months of exclusive breast-feeding. At the age of 12 months, none of the mothers was exclusively breast-feeding.
The age at the beginning of complementary feeding was not as variable as the DEB: first quartile, 144 d; median, 169 d; third quartile, 183 d. For 79 % of the infants, complementary feeding started before the first test session at the age of 6 months: for them, the median duration of complementary feeding was 33 d (first quartile, 14 d; third quartile, 54 d). At the age of 12 months, all infants were fed a solid diet, and the median duration of complementary feeding was 204 d (first quartile, 183 d; third quartile, 226 d).
Taste acceptance at 6 and 12 months
For some infants, IR data were missing for some specific tastes but all available IR were included in the analyses. Missing IR data happened for several reasons. First, some infants could not participate in a specific measurement session (resulting in a loss of six to eleven cases at the age 6 months and of sixteen to twenty-two cases at the age of 12 months). Second, some infants did not comply with the experimental procedure by refusing to drink from a bottle (two cases at the age of 6 months and three cases at the age of 12 months). Third, some infants did not complete the four-bottle sequence or did not meet the ingestion criteria (between one and nine cases at the age of 6 months and between two and seven cases at the age of 12 months).
Infants were first seen at the average age of 191 (sd 14) d (corrected age 181 (sd 8) d). The test sessions took place 130 (sd 49) min after the infant's last meal. On average, infants weighed 7·4 (sd 0·8) kg and were 66·2 (sd 5·9) cm long. At the age of 6 months, on average infants preferred sweet (IR 0·58 (sd 0·14); t 106= 5·72; P< 0·0001), salty (IR 0·55 (sd 0·11); t 110= 5·01; P< 0·0001) and umami (IR 0·53 (sd 0·11); t 105= 3·00; P= 0·0034) tastes over water. They were indifferent to sour and bitter tastes compared with water (IR 0·50 (sd 0·13); t 108= 0·36; P= 0·72; IR 0·49 (sd 0·09); t 105= − 1·54; P= 0·13, respectively).
For the taste acceptance assessment at the age of 12 months, infants were 371 (sd 15) d old (corrected age 362 (sd 8) d). The test sessions took place 129 (sd 53) min after the infant's last meal. On average, infants weighed 9·5 (sd 1·0) kg and were 74·6 (sd 2·9) cm long. At the age of 12 months, on average infants preferred sweet (IR 0·61 (sd 0·18); t 98= 6·04; P< 0·0001) and salty (IR 0·59 (sd 0·15); t 96= 5·47; P< 0·0001) tastes over water. They were indifferent to sour, bitter and umami tastes compared with water (IR 0·50 (sd 0·17); t 91= 0·09; P= 0·93; IR 0·48 (sd 0·12); t 99= − 1·29; P= 0·20; IR 0·50 (sd 0·16); t 94= 0·29; P= 0·77, respectively).
Paired t tests contrasting acceptance at 12 months and acceptance at 6 months did not reveal evolution with age for any taste: sweet taste (t 87= 1·61; P= 0·11; salty taste, t 90= 1·82; P= 0·07; sour taste, t 82= − 0·52; P= 0·60; bitter taste, t 89= − 0·66; P= 0·51; umami taste, t 85= − 1·59; P= 0·11).
Impact of the duration of exclusive breast-feeding on taste acceptance
At the age of 6 months, a positive correlation was observed between the DEB and umami taste acceptance (τ104= 0·16; P= 0·02; Fig. 1). No correlation was observed between the DEB and salty taste acceptance (τ109= − 0·02; P= 0·77). As it was hypothesised, no correlation was observed between the DEB and the acceptance of sweet, bitter or sour tastes (sweet taste: τ105= 0·04; P= 0·54; sour taste: τ107= 0·05; P= 0·47; bitter taste: τ104= 0·12; P= 0·06).
Fig. 1 Scatter plots of acceptance (ingestion ratio (IR)) at the age of 6 months of (a) sweet (n 107), (b) salty (n 111), (c) sour (n 109), (d) bitter (n 106) and (e) umami (n 106) taste solutions against duration of exclusive breast-feeding (DEB), and associated Kendall correlations ((a) τ = 0·04, (b) τ = − 0·02, (c) τ = 0·05, (d) τ = 0·12 and (e) τ = 0·16) and P values ((a) P= 0·54, (b) P= 0·77, (c) P= 0·47, (d) P= 0·06 and (e) P= 0·02). (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
At the age of 12 months, no significant correlation was observed between the DEB and taste acceptance (sweet taste: τ97= − 0·02; P= 0·82; salty taste: τ95= 0·05; P= 0·50; sour taste: τ90= − 0·13; P= 0·07; bitter taste: τ98= − 0·04; P= 0·55; umami taste: τ93= − 0·01; P= 0·87; Fig. 2).
Fig. 2 Scatter plots of acceptance (ingestion ratio (IR)) at the age of 12 months of (a) sweet (n 99), (b) salty (n 97), (c) sour (n 92), (d) bitter (n 100) and (e) umami (n 95) taste solutions against duration of exclusive breast-feeding (DEB), and associated Kendall correlations ((a) τ = − 0·02, (b) τ = 0·05, (c) τ = − 0·13, (d) τ = − 0·04 and (e) τ = − 0·01) and P values ((a) P= 0·82, (b) P= 0·05, (c) P= 0·07, (d) P= 0·55 and (e) P= 0·87). (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Discussion
The present study examined the impact of the DEB on taste acceptance at 6 and 12 months. At 6 months, as expected, longer exclusive breast-feeding had a positive impact on umami taste acceptance. This relationship was no longer observed at 12 months. No relationship was observed for salty taste at 6 or 12 months. Finally, with respect to our hypothesis, no correlation was observed between the DEB and sweet, sour and bitter taste acceptance at 6 or 12 months; however, two correlations approached significance: one positive between bitter taste acceptance at 6 months and DEB and one negative between sour taste acceptance at 12 months and DEB. At 6 months, infants preferred sweet, salty and umami solutions over water and were indifferent to sour and bitter solutions. At 12 months, infants preferred sweet and salty solutions over water and were indifferent to sour, bitter and umami solutions. The effects of some covariates (sex, difference between the expected and real birth date, duration since the introduction of complementary feeding) on the acceptance of basic tastes were considered in ANCOVA. However, none of any of the studied covariates had any effect on taste acceptance, for any age and any taste when taking into account, in the same model, the role of the DEB.
The observed association between the DEB and umami taste preference at 6 months, studied using monosodium glutamate, might be related to the effect of exposure to glutamate in breast milk as hypothesised. First, this association was modest. This might result from the variability in infant's behaviour in the taste acceptance measurement, or from the variability in infant's exposure to other foods. One might not exclude that introduction of complementary feeding could have an impact on taste acceptance(Reference Mennella, Forestell and Morgan22); however, the present analysis did not reveal an effect of the duration since the beginning of complementary feeding. Second, the interpretation of the present findings is limited by the fact that breast milk was neither analysed for taste compound composition, nor evaluated by a sensory panel to characterise its perceived taste. Thus, the exposure effect might only be interpreted in the light of previous findings. The possibility of an ‘imprinting’ effect of early exposure to glutamate in breast milk on the acceptance of umami flavour was previously raised on the basis of animal studies(Reference Mori, Kawada and Ono33, Reference Bellisle34). In 6-month-old human infants, an effect of exposure to different types of milk on taste acceptance has been shown(Reference Mennella, Forestell and Morgan22). Infants fed a hydrolysed casein formula, rich in amino acids, consumed more plain, umami-, bitter- or sour-tasting cereals than breast-fed infants or infants fed a regular formula. Infants fed a hydrolysed casein formula and breast-fed infants were more likely to smile when eating the umami-tasting cereal than infants fed a regular formula. These results were discussed in relation to the imprinting role of exposure to hydrolysed casein formula on further taste acceptance. Similarly, here, the 14-fold higher glutamate content in breast milk compared with that in formula milk(Reference Agostoni, Carratu and Boniglia19) might have led to a higher exposure to umami taste in breast-fed infants, resulting at 6 months in a higher acceptance of a umami solution prepared with monosodium glutamate. The 1·18 mmol/l glutamate content of breast milk(Reference Agostoni, Carratu and Boniglia19) is above the adult glutamate detection threshold, comprised between 0·08 and 0·39 mmol/l(Reference Lugaz, Pillias and Faurion35), and might result in a direct sensory exposure. The glutamate content in breast milk increases over the first trimester of lactation(Reference Jensen18, Reference Agostoni, Carratu and Boniglia28), and is relatively stable thereafter(Reference Yamawaki, Yamada and Kan-no36). This increase might be associated with the preference for umami taste at 6 months. The stability of breast milk glutamate content after 6 months and the progressive cessation of exclusive breast-feeding and initiation of complementary feeding in the second part of the first year(Reference Schwartz, Chabanet and Boggio37) might explain the absence of an association between breast-feeding duration and umami taste acceptance at 12 months. Altogether, the impact of exclusive breast-feeding on umami taste acceptance seems to be transient. It was observed at an age close to the beginning of complementary feeding and could favour the initial acceptance of umami-tasting foods with limited longer-term effect.
This could constitute a ‘taste bridge’ effect, in the same way that a ‘flavour bridge’ effect was previously described by Mennella and collaborators(Reference Mennella6, Reference Mennella, Jagnow and Beauchamp16) regarding flavour transition from breast milk to a solid diet. We showed elsewhere that between 5 and 7 months, the period corresponding to the beginning of complementary feeding, the more an infant liked umami, sweet or sour tastes, the more s/he accepted some of the foods bearing these tastes(Reference Schwartz, Chabanet and Lange38). It was speculated that the attraction to umami taste could derive from a specific ability to detect the presence of amino acids and proteins, which would be specifically efficient in protein- or amino-acid-deprived organisms. This was confirmed in amino acid-deficient animals(Reference Bellisle34) but not in malnourished infants(Reference Vasquez, Pearson and Beauchamp39). In healthy adults, taste threshold for monosodium glutamate combined with inosine 5′-monophosphate was found to be correlated with a liking for protein-rich foods(Reference Luscombe-Marsh, Smeets and Westerterp-Plantenga40). Therefore, the high glutamate content in breast milk may reflect a specific need for amino acids in primate infants(Reference Ramirez, DeSantiago and Tovar41, Reference Sarwar, Botting and Davis42), and in this view, the increased acceptance of umami taste following exclusive breast-feeding could be considered as a remarkable adaptation to guide the body towards foods bearing a specific nutritional function such as amino acids(Reference Jinap and Hajeb43). However, the specific role of glutamate in foods at this developmental stage remains to be elucidated. In this perspective, the metabolic role of glutamate in early development needs to be clarified, in particular its consequences on enteral functions(Reference Agostoni, Carratu and Boniglia19, Reference Blachier, Boutry and Bos44).
The slight preference (IR 0·53) for the glutamate solution compared with water at 6 months might seem surprising. Glutamate acceptance depends on the vehicle and on the concentration: in 3–24-month-old infants, adding 0·20 or 0·40 % glutamate to water reduced its ingestion, but not 0·05 or 0·10 %(Reference Beauchamp and Pearson45), whereas adding glutamate to a vegetable soup increased its ingestion(Reference Vasquez, Pearson and Beauchamp39). Adding glutamate to cereals did not have an impact on intake in 6-month-old formula- or breast-fed infants(Reference Mennella, Forestell and Morgan22). In line with the present slight preference for 0·17 % glutamate in water in 6-month-old infants and indifference in 12-month-old infants, one might consider the hypothesis of a concentration- and age-dependent preference for glutamate water solution.
Concerning salty taste, the present results did not corroborate previous findings(Reference Harris, Thomas and Booth23). The Na content of formula milks is about 2-fold higher than that of breast milk, whereas glutamate content in breast milk is 14-fold higher than in formula milks. This discrepancy could explain that the limited difference in exposure to salt resulting from longer breast-feeding did not result in a modified salt taste acceptance. Because most of the infants had already been receiving complementary foods by 6 months, any earlier difference in salt intake between formula- and breast-fed infants could have become blurred by this age.
The absence of the rejection of sour and bitter solutions might seem surprising. The possibility that some stimuli were not detected by some infants cannot be ruled out. However, infants exhibited negative facial expressions while drinking bitter and sour solutions (not reported here but discussed elsewhere(Reference Schwartz, Issanchou and Nicklaus27, Reference Schwartz, Issanchou and Nicklaus46)). Negative expressions and ingestion have also been reported in newborns tasting a urea solution(Reference Steiner32, Reference Ganchrow, Steiner and Daher47, Reference Desor, Maller and Andrews48). Adding citric acid to a mildly sweet solution was associated with a reduced ingestion in 2–24-month-old infants(Reference Vasquez, Pearson and Beauchamp39) but not its addition to water in newborns(Reference Desor, Maller and Andrews48). Moreover, in 6-month-old infants, 0·24 m-urea addition to cereals was not associated with a clear decrease in intake compared with plain cereal, neither was 0·006 m-citric acid addition(Reference Mennella, Forestell and Morgan22). Therefore, bitter and sour taste rejections do not seem to be systematic in infants.
A specific limitation to any study on breast-feeding is that it is not possible to carry out interventional studies randomising breast-feeding. Rate and duration of breast-feeding may vary according to several factors, in particular the mother's social status(Reference Gojard49, Reference Li, Darling and Maurice50). The observed effect of breast-feeding could be confounded with other factors related to the mother such as education. Here, such factors are unlikely to have directly affected the infant's behaviour during the sessions since taste acceptance was assessed using a double-blind procedure in which stimuli were delivered by the experimenter, not by the mother, and the outcome measure, the IR, was independent from the mother's judgement. To understand better the sensory consequences of exposure to breast milk, future studies on the development of taste and food preferences should focus on the analysis of breast milk composition, which has not been conducted here.
The present study highlighted the role of exclusive breast-feeding in the establishment of taste acceptance: longer exclusive breast-feeding has a positive impact on umami taste acceptance at the age of 6 months. Future studies should follow on studying the impact of breast-feeding on infants' acceptance of protein-rich foods such as meat, fish or cheese, or of foods such as tomatoes, mushrooms or peas in which glutamate content is higher than that of breast milk(Reference Ninomiya51), as well as the nutritional benefits of such foods at this age. Moreover, the sensory, behavioural and metabolic consequences of higher glutamate exposure in breast-fed infants deserve further exploration.
Acknowledgements
This study was funded by grants of the Regional Council of Burgundy, of IFR92, of the Nutrition, Chemical Food Safety and Consumer Behaviour Division of INRA, of PRNH-INRA-INSERM, of the ANR (The French National Research Agency) under the Programme National de Recherche en Alimentation et nutrition humaine, project ANR-06-PNRA-028, OPALINE; of the Benjamin Delessert Institute (Prix de Recherche 2008 to C. S.) and of the food-related companies Blédina, Nestlé, Symrise, CEDUS and Valrhona. The authors wish to thank the participating infants and parents; and the study experimenters: E. Szleper, J. Pierard and V. Feyen. The OPALINE team, in particular P. Schlich, C. Lange and S. Monnery-Patris, are thanked for their continuing support. C. S. designed the method, collected the sensory data and wrote the paper. C. C. analysed the data and contributed to the writing of the paper. C. L. recruited the subjects, collected the background data and organised the appointments. S. I. designed the method and wrote the paper. S. N. designed the method, collected the grant and wrote the paper. None of the authors has any conflict of interest to report.
