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The biological action of saponins in animal systems: a review

Published online by Cambridge University Press:  09 March 2007

George Francis ,
Zohar Kerem ,
Harinder P. S. Makkar  and
Klaus Becker
George Francis
Affiliation:
Department of Aquaculture Systems and Animal Nutrition, Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim (480), D 70593 Stuttgart, Germany
Zohar Kerem
Affiliation:
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot 76100, Israel
Harinder P. S. Makkar
Affiliation:
Animal Production and Health Section, International Atomic Energy Agency, P.O. Box 100, Wagramerstr. 5, A-1400 Vienna, Austria
Klaus Becker*
Affiliation:
Department of Aquaculture Systems and Animal Nutrition, Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim (480), D 70593 Stuttgart, Germany
*
*Corresponding author: Professor Dr K. Becker, fax +49 711 4593702, email [email protected]
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Abstract

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Saponins are steroid or triterpenoid glycosides, common in a large number of plants and plant products that are important in human and animal nutrition. Several biological effects have been ascribed to saponins. Extensive research has been carried out into the membrane-permeabilising, immunostimulant, hypocholesterolaemic and anticarcinogenic properties of saponins and they have also been found to significantly affect growth, feed intake and reproduction in animals. These structurally diverse compounds have also been observed to kill protozoans and molluscs, to be antioxidants, to impair the digestion of protein and the uptake of vitamins and minerals in the gut, to cause hypoglycaemia, and to act as antifungal and antiviral agents. These compounds can thus affect animals in a host of different ways both positive and negative.

Keywords

SaponinsSteroidsTriterpenoidsBiological activity
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 6 , December 2002 , pp. 587 - 605
Copyright
Copyright © The Nutrition Society 2002

References

Abe, H, Odashima, S & Arichi, S (1978 a) The effects of saikosaponins on biological membranes. 2. Changes in electron spin resonance spectra from spin labelled erythrocyte and erythrocyte ghost membranes. Planta Medica 34, 287–290.Google Scholar
Abe, H, Sakaguchi, M, Konishi, H, Tani, T & Arichi, S (1978 b) The effects of saikosaponins on biological membranes. 1. The relationship between the structures of saikosaponins and haemolytic activity. Planta Medica 34, 160–166.CrossRefGoogle ScholarPubMed
Al-Habori, M & Raman, A (1998) Antidiabetic and hypocholesterolaemic effects of fenugreek (Review). Phytotherapy Research 12, 233–242.3.0.CO;2-V>CrossRefGoogle Scholar
Almawi, WY & Melemedjian, OK (2002) Negative regulation of nuclear factor-kappa B activation and function by glucocorticoids. Journal of Molecular Endocrinology 28, 69–78.CrossRefGoogle ScholarPubMed
Andersen, CY (2002) Possible new mechanism of cortisol action in female reproductive organs: physiological implications of the free hormone hypothesis. Journal of Endocrinology 173, 211–217.CrossRefGoogle ScholarPubMed
Anderson, DP (1992) Immunostimulants, adjuvants and vaccine carriers in fish: applications to aquaculture. Annual Review of Fish Diseases 2, 281–307.CrossRefGoogle Scholar
Anthony, NB, Balog, JM, Staudinger, FB, Wall, CW, Walker, RD & Huff, WE (1994) Effects of a urease inhibitor and ceiling fans on ascites in broilers. 1. Environmental variability and incidence of ascites. Poultry Science 73, 801–809.CrossRefGoogle ScholarPubMed
Apers, S, Baronikova, S, Sindambiwe, JB, Witvrouw, M, De Clercq, E, Vanden Berghe, D, Van Marck, E, Vlietinck, A & Pieters, L (2000) Antiviral, haemolytic and molluscicidal activities of triterpenoid saponins fromaesa lanceolata: establishment of structure-activity relationships. MPlanta Medica 67, 528–532.CrossRefGoogle Scholar
Arletti, R, Benelli, A, Cavazzuti, E, Scarpetta, G & Bertolini, A (1999) Stimulating property of Turnera diffusa and Pfaffia paniculata extracts on the sexual behavior of male rats. Psychopharmacology 143, 15–19.CrossRefGoogle ScholarPubMed
Armah, CN, Mackie, AR, Roy, C, Price, K, Osbourn, AE, Bowyer, P & Ladha, S (1999) The membrane-permeabilising effect of avenacin A-1 involves the reorganization of bilayer cholesterol. Biophysical Journal 76, 281–290.CrossRefGoogle Scholar
Attele, AS, Wu, JA & Yuan, CS (1999) Ginseng pharmacology – Multiple constituents and multiple actions. Biochemical Pharmacology 58, 1685–1693.CrossRefGoogle ScholarPubMed
Authi, KS, Rao, GHR, Evenden, BJ & Crawford, N (1988) Action of guanosine 5'-(beta-thio)diphosphate on thrombin-induced activation and calcium mobilization in saponin-permeabilized and intact human platelets. Biochemical Journal 255, 885–894.CrossRefGoogle ScholarPubMed
Bae, EA, Park, SY & Kim, DH (2000) Constitutive beta-glucosidases hydrolyzing ginsenoside R-b1 and R-b2 from human intestinal bacteria. Biological and Pharmaceutical Bulletin 23, 1481–1485.CrossRefGoogle Scholar
Bangham, AD & Horne, RW (1962) Action of saponins on biological cell membranes. Nature 196, 952–953.CrossRefGoogle ScholarPubMed
Barr, IG, Sjolander, A & Cox, JC (1998) ISCOMs and other saponin based adjuvants (Review). Advanced Drug Delivery Reviews 32, 247–271.Google Scholar
Bei, L, Hu, TH, Qian, ZM & Shen, X (1998) Extracellular Ca2+ regulates the respiratory burst of human neutrophils. Biochimica et Biophysica Acta – Molecular Cell Research 1404, 475–483.CrossRefGoogle ScholarPubMed
Benie, T, El-Izzi, A, Tahiri, C, Duval, J & Thieulant, MLTI (1990) Combretodendron africanum bark extract as an antifertility agent. I: Estrogenic effects in vivo and LH release by cultured gonadotrope cells. Journal of Ethnopharmacology 29, 13–23.CrossRefGoogle ScholarPubMed
Bloom, BR (1989) Vaccines for the Third World. Nature 342, 115–116.CrossRefGoogle ScholarPubMed
Bomford, R, Stapleton, M, Winsor, S, Beesley, JE, Jessup, EA, Price, KR & Fenwick, GR (1992) Adjuvanticity and ISCOM formation by structurally diverse saponins. Vaccine 10, 572–577.Google Scholar
Bosler, DA, Blümmel, M, Bullerdieck, P, Makkar, HPS & Becker, K (1997) Influence of a saponin containing feed additive on mass development and carcass evaluation of growing lambs. Proceedings of the Society of Nutrition and Physiology 6, 46.Google Scholar
Brain, K, Hadgraft, J & Al-Shatalebi, M (1990) Membrane modification in activity of plant molluscicides. Planta Medica 56, 663.Google Scholar
Bureau, DP, Harris, AM & Cho, CY (1998) The effects of purified alcohol extracts from soy products on feed intake and growth of chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss). Aquaculture 161, 27–43.CrossRefGoogle Scholar
Cai, J, Liu, M, Wang, Z & Ju, Y (2002) Apoptosis induced by dioscin in Hela cells. Biological and Pharmaceutical Bulletin 25, 193–196.CrossRefGoogle ScholarPubMed
Calvert, GD & Blight, L (1981) A trial of the effects of soya-bean flour and soya-bean saponins on plasma lipids, fecal bile acids and neutral sterols in hypercholesterlaemic men. British Journal of Nutrition 45, 277–281.Google Scholar
Chao, AC, Nguyen, JV, Broughall, M, Recchia, J, Kensil, CR, Daddona, PE & Fix, JA (1998) Enhancement of intestinal model compound transport by DS-1, a modified. Quillaja saponin. Journal of Pharmaceutical Sciences 87, 1395–1399.CrossRefGoogle ScholarPubMed
Chavali, SR, Francis, T & Campbell, JB (1987) An in vitro study of immunodilatory effects of some saponins. International Journal of Immunopharmacology 9, 675–683.CrossRefGoogle Scholar
Cheeke, PR (1996) Biological effects of feed and forage saponins and their impact on animal production. In Saponins Used in Food and Agriculture, pp. 377–386 [Waller, GR and Yamasaki, Y, editors]. New York: Plenum PressGoogle Scholar
Chen, JC & Chen, KW (1997) Oxygen uptake and ammonia-N excretion of juvenile. Penaeus japonicus during depuration following one-day exposure to different concentrations of saponin at different salinity levels. Aquaculture 156, 77–83.CrossRefGoogle Scholar
Chen, JC, Xu, MX, Chen, LD, Chen, YN & Chiu, TH (1998) Effect of. Panax notoginseng saponins on sperm motility and progression in vitro. Phytomedicine 5, 289–292.Google Scholar
Choi, S, Jung, SY, Kim, CH, Kim, HS, Rhim, H, Kim, SC & Nah, SY (2001) Effect of Ginsenosides on voltage-dependent Ca2+ channel subtypes in bovine chromaffin cells. Journal of Ethnopharmacology 74, 75–81.CrossRefGoogle ScholarPubMed
Chou, SC, Ramanathan, S, Matsui, A, Rojers, J & Cutting, WC (1971) Isolation of saponins with antifertility activity from Gleditschia horrida. Indian Journal of Experimental Biology 9, 503–504.Google ScholarPubMed
Chung, E, Lee, KY, Lee, YJ, Lee, YH & Lee, SK (1998) Ginsenoside Rg1 down-regulates glucocorticoid receptor and displays synergistic effects with cAMP. Steroids 63, 421–424.CrossRefGoogle ScholarPubMed
Cossarni-Dunier, M (1985) Effect of different adjuvants on the humoral immune response of rainbow trout. Developmental and Comparative Immunology 9, 141–146.CrossRefGoogle Scholar
Coulter, A, Wong, TY, Drane, D, Bates, J, Macfarlan, R & Cox, J (1998) Studies on experimental adjuvanted influenza vaccines: comparison of immune stimulating complexes (Iscoms (TM)) and oil-in-water vaccines. Vaccine 16, 1243–1253.Google Scholar
de Kloet, ER, Reul, JM, van den Bosch, FR, Tonnaer, JA & Saito, H (1987) Ginsenoside RG1 and corticosteroid receptors in rat brain. Endocrinology Japan 34, 213–220.CrossRefGoogle ScholarPubMed
Delmas, F, Di Giorgio, C, Elias, R, Gasquet, M, Azas, N, Mshvildadze, V, Dekanosidze, G, Kemertelidze, E & Timon-David, P (2000) Antileishmanial activity of three saponins isolated from ivy, alpha-hederin, beta-hederin and hederacolchiside A(1), as compared with their action on mammalian cells cultured in vitro. Planta Medica 66, 343–347.CrossRefGoogle Scholar
DeMeo, MT, Mutlu, EA, Keshavarzian, A & Tobin, MC (2002) Intestinal Permeation and Gastrointestinal Disease. Journal of Clinical Gastroenterology 34, 385–396.CrossRefGoogle ScholarPubMed
Deng, HL & Zhang, JT (1991) Anti-lipid peroxilative effect of ginsenoside Rb1 and Rg1. Chinese Medical Journal 104, 395–398.Google ScholarPubMed
de Oliveira, CAC, Perez, AC, Merino, G, Prieto, JG & Alvarez, AI (2001) Protective effects of. Panax ginseng on muscle injury and inflammation after eccentric exercise. Comparative Biochemistry and Physiology 130C, 369–377.Google Scholar
Dollahite, JW, Shaver, T & Camp, BJ (1962) Injected saponins as abortifacients. American Journal of Veterinary Research 23, 1261–1263.Google Scholar
Dorsaz, A-C, Hostettmann, M & Hostettmann, K (1988) Molluscicidal saponins from. Sesbania sesban. Planta Medica 54, 225–227.Google Scholar
El Izzi, A, Benie, T, Thieulant, M-L, Le Men-Oliver, L & Duval, J (1992) Stimulation of LH release from cultured pituitary cells by saponins of Petersianthus macrocarpus: a permeabilising effect. Planta Medica 58, 229–233.CrossRefGoogle Scholar
El Izzi, A, Duval, J & Delaude, C (1989) Effet d'une serie de saponines extrites de vegetaux de L'Afrique tropicale sur la liberation d'hormone luteinisante par les cellules hypophysaires en culture (Effect of a series of saponin extracts from African plants on the release of luteinizing hormone by hypophysial cells in culture). Bulletin de la Societé Royale des Sciences de Liege 58, 53–56.Google Scholar
Enomoto, Y, Ito, K, Kawagoe, Y, Morio, Y & Yamasaki, Y (1986) Positive inotropic action of saponins on isolated atrial and papillary muscles from the guinea pig. British Journal of Pharmacology 88, 259–267.CrossRefGoogle ScholarPubMed
Fenwick, GR, Price, KR, Tsukamoto, C & Okubo, K (1991) Saponins. In Saponins in Toxic Substances in Crop Plants, [D'Mello, FJP, Duffus, CM and Duffus, JH, editors]. Cambridge: The Royal Society of ChemistryGoogle Scholar
Flaoyen, A, Wilkins, AL, Deng, D & Brekke, T (2001) Ovine metabolism of saponins: Evaluation of a method for estimating the ovine uptake of steroidal saponins from Narthecium ossifragum. Veterinary Research Communications 25, 225–238.Google Scholar
Francis, G, Makkar, HPS & Becker, K (2001 a) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199, 197–227.CrossRefGoogle Scholar
Francis, G, Makkar, HPS & Becker, K (2001 b) Effects of Quillaja saponins on growth, metabolism, egg production, and muscle cholesterol in individually reared Nile tilapia (Oreochromis niloticus). Comparative Biochemistry and Physiology 129C, 105–114.Google Scholar
Francis, G, Makkar, HPS & Becker, K (2002 a) Dietary supplementation with a Quillaja saponin mixture improves growth performance and metabolic efficiency in common carp (Cyprinus carpio L). Aquaculture 203, 311–320.CrossRefGoogle Scholar
Francis, G, Makkar, HPS & Becker, K (2002 b) Effects of cyclic and regular feeding of Quillaja saponin supplemented diet on growth and metabolism of common carp (Cyprinus carpio L). Fish Physiology and Biochemistry 24, 343–350.CrossRefGoogle Scholar
Fukuda, K, Utsumi, H, Shoji, J & Hamada, A (1985) Saponins can cause the agglutination of phospholipid vesicles. Biochimica et Biophysica Acta 820, 199–206.CrossRefGoogle ScholarPubMed
Gagliardo, R, Vignola, AM & Mathieu, M (2001) Is there a role for glucocorticoid receptor beta in asthma? Respiratory Research 2, 1–4.CrossRefGoogle Scholar
Gee, JM & Johnson, IT (1988) Interactions between haemolytic saponins, bile salts and small intestinal mucosa in the rat. Journal of Nutrition 118, 1391–1397.CrossRefGoogle ScholarPubMed
Gee, JM, Price, KR, Johnson, IT & Rhodes, MJ (1998) The relationship between saponin structure and bioactivity – a preliminary study. In Cost 98, Effects of Antinutrients on the Nutritional Value of Legume Diets no. 4, pp. 8–14 [Bardocz, S & Pusztai, A, editors]. Luxemburg: European CommissionGoogle Scholar
Gee, JM, Price, KR, Ridout, CL, Johnson, IT & Fenwick, GR (1989) Effects of some purified saponins on transmural potential difference in mammalian small intestine. Toxicology In Vitro 3, 85–90.CrossRefGoogle ScholarPubMed
Gee, JM, Price, KR, Ridout, CL, Wortley, GM, Hurrell, RF & Johnson, IT (1993) Saponins of quinoa (Chenopodium quinoa): Effects of processing on their abundance in quinoa products and their biological effects on intestinal mucosal tissue. Journal of the Science of Food and Agriculture 63, 201–209.CrossRefGoogle Scholar
Gee, JM, Wal, JM, Miller, K, Atkinson, H, Grigoriadou, F, Wijnands, MVW, Penninks, AH, Wortley, G & Johnson, IT (1997) Effect of saponin on the transmucosal passage of β-lactoglobulin across the proximal small intestine of normal and β-lacoglobulin-sensitised rats. Toxicology 117, 219–228.CrossRefGoogle ScholarPubMed
Gee, JM, Wortley, GM, Johnson, IT, Price, KR, Rutten, AAJJL, Houben, GF & Penninks, AH (1996) Effect of saponins and glycoalkaloids on the permeability and viability of mammalian intestinal cells and on the integrity of tissue preparations in vitro. Toxicology In Vitro 10, 117–128.CrossRefGoogle ScholarPubMed
Glauert, AM, Dingle, JT & Lucy, JA (1962) Action of saponin on biological membranes. Nature 196, 953–955.CrossRefGoogle Scholar
Gögelein, H & Hüby, A (1984) Interaction of saponin and digitonin with black lipid membranes and lipid monolayers. Biochimica et Biophysica Acta 773, 32–38.CrossRefGoogle ScholarPubMed
Grayson, TH, Williams, RJ, Wrathmell, AB, Munn, CB & Harris, JE (1987) Effects of immunopotentiating agents on the immune response of rainbow trout,. Salmo gairdneri Richardson, to ERM vaccine. Journal of Fish Biology 31, Suppl. A, 195–202.CrossRefGoogle Scholar
Gus-Mayer, S, Brunner, H, Schneider-Poetsch, HA & Rudiger, W (1994) Avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the BGA family of beta-glucosidases. Plant Molecular Biology 26, 909–921.CrossRefGoogle ScholarPubMed
Hamburger, M, Slacanin, I, Hostettmann, K, Dyatmiko, W & Sutarjadi, (1992) Acetylated saponins with molluscicidal activity from Sapindus rarak: unambiguous structure determination by proton nuclear magnetic resonance and quantitative analysis. Phytochemical Analysis 3, 231–237.CrossRefGoogle Scholar
Han, LK, Xu, BJ, Kimura, Y, Zheng, YN & Okuda, H (2000) Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. Journal of Nutrition 130, 2760–2764.CrossRefGoogle ScholarPubMed
Hanausek, M, Ganesh, P, Walaszek, Z, Arntzen, CJ, Slaga, TJ & Gutterman, JU (2001) Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), suppress H-ras mutations and aneuploidy in a murine skin carcinogenesis model. Proceedings of the National Academy of Sciences USA 98, 11551–11556.CrossRefGoogle Scholar
Haralampidis, K, Trojanowska, M & Osbourn, AE (2002) Biosynthesis of triterpenoid saponins in plants. Advances in Biochemical Engineering/Biotechnology 75, 31–49.Google Scholar
Haridas, V, Arntzen, CJ & Gutterman, JU (2001 a) Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), inhibit activation of nuclear factor-kappa B by inhibiting both its nuclear localization and ability to bind DNA. Proceedings of the National Academy of Sciences USA 98, 11557–11562.Google Scholar
Haridas, V, Higuchi, M, Jayatilake, GS, Bailey, D, Mujoo, K, Blake, ME, Arntzen, CJ & Gutterman, JU (2001 b) Avicins: triterpenoid saponins from Acacia victoriae (Bentham) induce apoptosis by mitochondrial perturbation. Proceedings of the National Academy of Sciences USA 98, 5821–5826.CrossRefGoogle ScholarPubMed
Harris, WS, Dujovne, CA, Windsor, SL, Gerrond, LLC, Newton, FA & Gelfand, RA (1997) Inhibiting cholesterol absorption with CP-88,818 (beta-tigogenin cellobioside; tiqueside): Studies in normal and hyperlipidemic subjects. Journal of Cardiovascular Pharmacology 30, 55–60.Google Scholar
Harwood, HJ Jr, Chandler, CE, Pellarin, LD, Bangerter, FW, Wilkins, RW, Long, CA, Cosgrove, PG, Malinow, MR, Marcetta, CA, Pettini, JL, Savoy, YE & Mayne, JT (1993) Pharmacologic consequences of cholesterol absorption inhibition: alteration in cholesterol metabolism and reduction in plasma cholesterol concentration induced by the synthetic saponin β-tigogenin cellobioside (CP-88818; tiqueside). Journal of Lipid Research 34, 377–395.CrossRefGoogle ScholarPubMed
Hu, J, Lee, SO, Hendrich, S & Murphy, PA (2002) Quantification of the group B soyasaponins by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 50, 2587–2594.CrossRefGoogle Scholar
Hu, M, Konoki, K & Tachibana, K (1996) Cholesterol-independent membrane disruption caused by triterpenoid saponins. Biochimica et Biophysica Acta – Lipid Metabolism 1299, 252–258.CrossRefGoogle ScholarPubMed
Hussain, I & Cheeke, PR (1995) Effect of Yucca scidigera extract on rumen and blood profiles of steers fed concentrate- or roughage-based diets. Animal Feed Science and Technology 51, 231–242.CrossRefGoogle Scholar
Ikedo, S, Shimoyamada, M & Watanabe, K (1996) Interaction between bovine serum albumin and saponin as studied by heat stability and protease digestion. Journal of Agricultural and Food Chemistry 44, 792CrossRefGoogle Scholar
Ishaaya, I, Birk, Y, Bondi, A & Tencer, Y (1969) Soyabean saponins. IX. Studies of their effect on birds, mammals and cold-blooded organisms. Journal of the Science of Food and Agriculture 20, 433–436.Google Scholar
Ishisaki, S, Iwase, M & Tanaka, M (1997) Enhancing effect of starfish Asterias amurensis saponin upon thermal aggregation of actomyosin from alleye pollack meat. Fisheries Science 63, 159–160.CrossRefGoogle Scholar
Jenkins, KJ & Atwal, AS (1994) Effects of dietary saponins on fecal bile acids and neutral sterols, and availability of vitamins A and E in the chick. Journal of Nutritional Biochemistry 5, 134–138.CrossRefGoogle Scholar
Jenkins, PG, Harris, JE & Pulsford, AL (1991) Enhanced enteric uptake of human gamma globulin by Quil-A saponin in Oreochromis mossambicus. Fish and Shellfish Immunology 1, 279–295.CrossRefGoogle Scholar
Jie, YH, Cammisuli, S & Baggiolini, M (1984) Immunomodulatory effects of Panax ginseung C. A. MEYER in the mouse. Agents and Actions 15, 386–391.CrossRefGoogle Scholar
Johnson, IT, Gee, JM, Price, K, Curl, C & Fenwick, GR (1986) Influence of saponins on gut permeability and active nutrient transport in vitro. Journal of Nutrition 116, 2270–2277.CrossRefGoogle ScholarPubMed
Johnston, NL, Quarles, CL & Fagerberg, DJ (1982) Broiler performance with DSS40 Yucca saponin in combination with monensin. Poultry Science 61, 1052–1054.CrossRefGoogle Scholar
Johnston, NL, Quarles, CL, Fagerberg, DJ & Caveny, DD (1981) Evaluation of Yucca saponin on broiler performance and ammonia suppression. Poultry Science 60, 2289–2292.CrossRefGoogle Scholar
Kai, L, Wang, ZF & Xiao, JS (1998) L-type calcium channel blockade mechanisms of panaxadiol saponins against anoxic damage of cerebral cortical neurons isolated from rats. Acta Pharmacologica Sinica 19, 455–458.Google Scholar
Kensil, CR (1996) Saponins as vaccine adjuvants. Critical Reviews in Therapeutic Drug Carrier Systems 13, 1–55.Google ScholarPubMed
Killeen, GF, Madigan, CA, Connolly, CR, Walsh, GA, Clark, C, Hynes, MJ, Timmins, BF, James, P, Headon, DR & Power, RF (1998) Antimicrobial saponins of Yucca Schidigera and the implications of their in vitro properties for their in vivo impact. Journal of Agricultural and Food Chemistry 46, 3178–3186.CrossRefGoogle Scholar
Kim, DH, Jung, JS, Suh, HW, Huh, SO, Min, SK, Son, BK, Park, JH, Kim, ND, Kim, YH & Song, DK (1998 a) Inhibition of stress-induced plasma corticosterone levels by Ginsenosides in mice: involvement of nitric oxide. Neuroreport 9, 2261–2264.CrossRefGoogle ScholarPubMed
Kim, DW, Bang, KH, Rhee, YH, Lee, KT & Park, HJ (1998 b) Growth inhibitory activities of kalopanaxsaponins A and I against human pathogenic fungi. Archives of Pharmacal Research 21, 688–691.CrossRefGoogle ScholarPubMed
Kim, SJ, Kim, YY, Ko, KH, Hong, EK, Han, YB, Kang, BH & Kim, H (1998 c) Butanol extract of 1: 1 mixture of Phellodendron cortex and Aralia cortex stimulates PI3-kinase and ERK2 with increase of glycogen levels in HepG2 cells. Phytotherapy Research 12, 255–260.3.0.CO;2-9>CrossRefGoogle Scholar
Kim, YH, Park, KH & Rho, HM (1996) Transcriptional activation of the Cu, Zn-superoxide dismutase gene through the AP2 site by ginsenoside Rb2 extracted from a medicinal plant, Panax ginseng. Journal of Biological Chemistry 271, 24539–24543.CrossRefGoogle ScholarPubMed
Kim, YR, Lee, SY, Shin, BA & Kim, KM (1999) Panax ginseng blocks morphine-induced thymic apoptosis by lowering plasma corticosterone level. General Pharmacology 32, 647–652.Google Scholar
Kishor, N & Sati, OP (1990) A new molluscicidal spirostanol glycoside of Yucca alufolia. Journal of Natural Products 53, 1557–1559.Google Scholar
Klita, PT, Mathison, GW & Fenton, TW (1996) Effect of alfalfa root saponins on digestive function in sheep. Journal of Animal Science 74, 1144–1156.Google Scholar
Konoshima, T, Takasaki, M, Tokuda, H, Nishino, H, Duc, NM, Kasai, R & Yamasaki, K (1998) Anti-tumor-promoting activity of majonoside-R2 from Vietnamese ginseng, Panax vietnamensis HA et GRUSHV. (I). Biological and Pharmaceutical Bulletin 21, 834–838.CrossRefGoogle Scholar
Krogdahl, A, Roem, A & Baeverfjord, G (1995) Effects of soybean saponin, raffinose and soybean alcohol extract on nutrient digestibilities, growth and intestinal morphology in Atlantic salmon. In Quality in Aquaculture. Proceedings of the International Conference of Aquaculture '95 and the Satellite Meeting Nutrition and Feeding of Cold Water Species, Trondheim, Norway, 9–12 August 1995. European Aquaculture Society Special Publication no 23, pp. 118–119 [Svennevig, N and Krogdahl, A, editors]. Gent, Belgium: European Aquaculture SocietyGoogle Scholar
Kuroda, M, Mimaki, Y, Hasegawa, F, Yokosuka, A, Sashida, Y & Sakagami, H (2001) Steroidal glycosides from the bulbs of Camassia leichtlinii and their cytotoxic activities. Chemical and Pharmaceutical Bulletin 49, 726–731.CrossRefGoogle ScholarPubMed
Kuznetzova, TA, Anisimov, MM & Popov, AM (1982) A comparative study in vitro of physiological activity of triterpene glycosides of marine invertebrates of echinoderm type. Comparative Biochemistry and Physiology 73C, 41–43.Google Scholar
Lacaille-Dubois, MA, Hanquet, B, Cui, ZH, Lou, ZC & Wagner, H (1999) A new biologically active acylated triterpene saponin from Silene fortunei. Journal of Natural Products 62, 133–136.CrossRefGoogle ScholarPubMed
Lee, YJ, Chung, E, Lee, KY, Lee, YH, Huh, B & Lee, SK (1997) Ginsenoside-Rg1, one of the major active molecules from Panax ginseng, is a functional ligand of glucocorticoid receptor. Molecular and Cellular Endocrinology 133, 135–140.CrossRefGoogle ScholarPubMed
Lee, KT, Sohn, IC, Kim, DH, Choi, JW & Kwon, SH (2000 a) Hypoglycaemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activity in vitro. Archives of Pharmacal Research 23, 461–466.CrossRefGoogle ScholarPubMed
Lee, KT, Sohn, IC, Park, HJ, Kim, DW & Jung, GO (2000 b) Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides and bisdesmosides isolated from the stem bark of Kalopanax pictus. Planta Medica 66, 329–332.CrossRefGoogle ScholarPubMed
Lee, SJ, Ko, WG, Kim, JH, Sung, JH, Lee, SJ, Moon, CK & Lee, BH (2000 c) Induction of apoptosis by a novel intestinal metabolite of ginseng saponin via cytochrome c-mediated activation of caspase-3 protease. Biochemical Pharmacology 60, 677–685.CrossRefGoogle ScholarPubMed
Lee, SJ, Son, KH, Chang, HW, Kang, SS & Kim, HP (1998) Antiinflammatory activity of Lonicera japonica. Phytotherapy Research 12, 445–447.3.0.CO;2-5>CrossRefGoogle Scholar
Lemma, A (1965) A preliminary report on the molluscicidal property of endod (Phytolacca dodecandra). Ethiopian Medical Journal 3, 187.Google Scholar
Liu, WK, Xu, SX & Che, CT (2000) Anti-proliferative effect of ginseng saponins on human prostate cancer cell line. Life Sciences 67, 1297–1306.Google Scholar
Lu, CD & Jorgensen, NA (1987) Alfalfa saponins affect site and extent of nutrient digestion in ruminants. Journal of Nutrition 117, 919–927.CrossRefGoogle ScholarPubMed
Ma, LY & Xiao, PG (1998) Effects of Panax notoginseng saponins on platelet aggregation in rats with middle cerebral artery occlusion or in vitro and on lipid fluidity of platelet membrane. Phytotherapy Research 12, 138–140.3.0.CO;2-C>CrossRefGoogle Scholar
McAllister, TA, Annett, CB, Cockwill, CL, Olson, ME, Wang, Y & Cheeke, PR (2001) Studies on the use of Yucca schidigera to control giardiosis. Veterinary Parasitology 97, 85–99.CrossRefGoogle ScholarPubMed
McGhee, JR, Mestecky, J, Dertzbaugh, MT, Eldridge, JH, Hirasawa, M & Kiyono, H (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10, 75–88.Google Scholar
McManus, OB, Harris, GH & Giangiacombo, KM (1993) An activator of calcium-dependent potassium channels isolated from a medicinal herb. Biochemistry 32, 6128–6133.Google Scholar
Mader, TL & Brumm, MC (1987) Effect of feeding sarsasaponin in cattle and swine diets. Journal of Animal Science 65, 9–15.CrossRefGoogle Scholar
Maharaj, K, Froh, KH & Campbell, JB (1986) Immune responses of mice to inactivated rabies vaccine administered orally: potentiation by Quillaja saponin. Canadian Journal of Microbiology 32, 414–420.Google Scholar
Makkar, HPS, Aregheore, EM & Becker, K (1999) Effects of saponins and plant extracts containing saponins on the recovery of ammonia during urea ammoniation of wheat straw and fermentation kinetics of the treated straw. Journal of Agricultural Science, Cambridge 132, 313–321.Google Scholar
Makkar, HPS & Becker, K (1996) Effect of Quillaja saponins on in vitro rumen fermentation. In Saponins Used in Food and Agriculture, pp. 377–386 [Waller, GR and Yamasaki, Y, editors]. New York: Plenum PressGoogle Scholar
Marino, SD, Iorizzi, M, Palagiano, E, Zollo, F & Roussakis, C (1998) Starfish saponins. 55. Isolation, structure elucidation, and biological activity of steroid oligoglycosides from an antarctic starfish of the family Asteriidae. Journal of Natural Products 61, 1319–1327.CrossRefGoogle ScholarPubMed
Marston, A, Wolfender, JL & Hostettmann, K (2000) Analysis and isolation of saponins from plant material. In Saponins in Food, Feedstuffs and Medicinal Plants, Annual Proceedings of the Phytochemical Society, pp. 1–12 [Oleszek, W and Marston, A, editors]. Oxford and London: Clarendon Press.Google Scholar
Matsuda, H, Li, Y, Yamahara, J & Yoshikawa, M (1999 a) Inhibition of gastric emptying by triterpene saponin, momordin Ic, in mice: Roles of blood glucose, capsaicin-sensitive sensory nerves, and central nervous system. Journal of Pharmacology and Experimental Therapeutics 289, 729–734.Google ScholarPubMed
Matsuda, H, Li, YH, Murakami, T, Yamahara, J & Yoshikawa, M (1999 b) Structure-related inhibitory activity of oleanolic acid glycosides on gastric emptying in mice. Bioorganic and Medicinal Chemistry 7, 323–327.CrossRefGoogle ScholarPubMed
Matsuda, H, Murakami, K, Ninomiya, K, Yamahara, J & Yoshikawa, M (1997) Effects of escins 1a, 1b, 11a, and 11b from horse chestnut, the seeds of Aesculus hippocastanum L, on acute inflammation in animals. Chemical and Pharmaceutical Bulletin 20, 1092–1095.Google Scholar
Matsuura, M (2001) Saponins in garlic as modifiers of the risk of cardiovascular disease. Journal of Nutrition 131, 1000S–1005S.CrossRefGoogle ScholarPubMed
Meagher, LP, Smith, BL & Wilkins, AL (2001) Metabolism of diosgenin-derived saponins: implications for hepatogenous photosensitization diseases in ruminants. Animal Feed Science and Technology 91, 157–170.CrossRefGoogle Scholar
Melzig, MF, Bader, G & Loose, R (2001) Investigations of the mechanism of membrane activity of selected triterpenoid saponins. Planta Medica 67, 43–48.CrossRefGoogle ScholarPubMed
Mengoni, F, Lichtner, M, Battinelli, L, Marzi, M, Mastroianni, CM, Vullo, V & Mazzanti, G (2002) In vitro anti-HIV activity of oleanolic acid on infected human mononuclear cells. Planta Medica 68, 111–114.Google Scholar
Menin, L, Panchichkina, M, Keriel, C, Olivares, J, Braun, U, Seppert, EK & Saks, VA (2001) Macrocompartmentation of total creatine in cardiomyocytes revisited. Molecular and Cellular Biochemistry 220, 149–159.CrossRefGoogle ScholarPubMed
Menzies, GS, Howland, K, Rae, MT & Bramley, TA (1999) Stimulation of specific binding of [3H]-progesterone to bovine luteal cell-surface membranes: specificity of digitonin. Molecular and Cellular Endocrinology 153, 57–69.Google Scholar
Mimaki, Y, Kuroda, M, Kameyama, A, Yokosuka, A & Sashida, Y (1998a) Steroidal saponins from the underground parts of Ruscus aculeatus and their cytostatic activity on HL-60 cells. Phytochemistry 48, 485–493.Google Scholar
Mimaki, Y, Kuroda, M, Kameyama, A, Yokosuka, A & Sashida, Y (1998b) Steroidal saponins from the rhizomes of Hosta sieboldii and their cytostatic activity on HL-60 cells. Phytochemistry 48, 1361–1369.CrossRefGoogle ScholarPubMed
Mimaki, Y, Yokosuka, A, Kuroda, M & Sashida, Y (2001) Cytotoxic activities and structure-cytotoxic relationships of steroidal saponins. Biological and Pharmaceutical Bulletin 24, 1286–1289.CrossRefGoogle ScholarPubMed
Miyakoshi, M, Tamura, Y, Masuda, H, Mizutani, K, Tanaka, O, Ikeda, T, Ohtani, K, Kasai, R & Yamasaki, K (2000) Antiyeast steroidal saponins from Yucca schidigera (Mohave yucca), a new anti-food-deteriorating agent. Journal of Natural Products 63, 332–338.Google Scholar
Monder, C, Stewart, PM, Lakshmi, V, Valentino, R, Burt, D & Edwards, CRW (1989) Licorice inhibits corticosteroid 11β-dehydrogenase of rat kidney and liver: in vivo and in vitro studies. Endocrinology 125, 1046–1053.Google Scholar
Morehouse, LA, Bangerter, FW, DeNinno, MP, Inskeep, PB, McCarthy, PA, Pettini, JL, Savoy, YE, Sugarman, ED, Wilkins, RW, Wilson, TC, Woody, HA, Zaccaro, LM & Chandler, CE (1999) Comparison of synthetic saponin cholesterol absorption inhibitors in rabbits: evidence for a non-stoichiometric, intestinal mechanism of action. Journal of Lipid Research 40, 464–474.Google Scholar
Morrissey, JP & Osbourn, AE (1999) Fungal resistance to plant antibiotics as a mechanism of pathogenesis. Microbiological and Molecular Biological Reviews 63, 708–724.CrossRefGoogle ScholarPubMed
Mowat, AM, Smith, RE, Donachie, AM, Furrie, E, Grdic, D & Lycke, N (1999) Oral vaccination with immune stimulating complexes. Immunology Letters 65, 133–140.Google Scholar
Muir, AD, Ballantyne, KD & Hall, TW (2000) LC-MS and LC-MS/MS analysis of saponins and sapogenins – comparison of ionization techniques and their usefulness in compound identification. In Saponins in Food, Feedstuffs and Medicinal Plants, Annual Proceedings of the Phytochemical Society, pp. 35–42 [Oleszek, W and Marston, A, editors]. Oxford and London: Clarendon PressCrossRefGoogle Scholar
Mujoo, K, Haridas, V, Hoffmann, JJ, Wachter, GA, Hutter, LK, Lu, Y, Blake, ME, Jayatilake, GS, Bailey, D, Mills, GB & Gutterman, JU (2001) Triterpenoid saponins from Acacia victoriae (Bentham) decrease tumor cell proliferation and induce apoptosis. Cancer Research 61, 5486–5490.Google ScholarPubMed
Murakami, T, Nakamura, J, Matsuda, H & Yoshikawa, M (1999) Bioactive saponins and glycosides. XV. Saponin constituents with gastroprotective effect from the seeds of tea plant, Camellia sinensis L var. assamica Pierre, cultivated in Sri Lanka: Structures of assamsaponins A, B, C, D, and E. Chemical and Pharmaceutical Bulletin 47, 1759–1764.CrossRefGoogle Scholar
Namba, T, Yoshsaki, M, Tomimori, T, Kobashi, K, Mitsui, K & Hase, J (1973) Haemolytic and protective activity of ginseng saponins. Chemical and Pharmaceutical Bulletin 21, 459–461.CrossRefGoogle ScholarPubMed
Newbold, CJ, El Hassan, SM, Wang, J, Ortega, ME & Wallace, RJ (1997) Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. British Journal of Nutrition 78, 237–249.CrossRefGoogle ScholarPubMed
Nguyen, TD, Villard, PH, Barlatier, A, Elsisi, AE, Jouve, E, Duc, NM, Sauze, C, Durand, A & Lacarelle, B (2000) Panax vietnamensis protects mice against carbon tetrachloride-induced hepatotoxicity without any modification of CYP2E1 gene expression. Planta Medica 66, 714–719.CrossRefGoogle ScholarPubMed
Oakenfull, DG (1986) Aggregation of bile acids and saponins in aqueous solution. Australian Journal of Chemistry 39, 1671–1683.CrossRefGoogle Scholar
Oakenfull, DG & Sidhu, GS (1990) Could saponins be a useful treatment for hypercholesterolemia? European Journal of Clinical Nutrition 44, 79–88.Google Scholar
Oda, K, Matsuda, H, Murakami, T, Katayama, S, Ohgitani, T & Yoshikawa, M (2000) Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants. Biological Chemistry 381, 67–74.Google Scholar
Odenyo, AA, Osuji, PO & Karanfil, O (1997) Effects of multipurpose tree (MPT) supplements on ruminant ciliate protozoa. Animal Feed Science and Technology 67, 169–180.CrossRefGoogle Scholar
Oh, YJ & Sung, MK (2001) Soybean saponins inhibit cell proliferation by suppressing PKC activation and induce differentiation of HT-29 human colon adenocarcinoma cells. Nutrition and Cancer 39, 132–138.Google Scholar
Ohana, P, Delmer, DP, Carlson, RW, Glushka, J, Azadi, P, Bacic, T & Benziman, M (1998) Identification of a novel triterpenoid saponin from Pisum sativum as a specific inhibitor of the diguanylate cyclase of Acetobacter xylinum. Plant and Cell Physiolology 39, 144–152.CrossRefGoogle ScholarPubMed
Oleszek, W, Nowacka, J, Gee, JM, Wortley, G & Johnson, IT (1994) Effects of some purified alfalfa (Medicago sativa) saponins on transmural potential difference in mammalian small intestine. Journal of the Science of Food and Agriculture 65, 35–39.CrossRefGoogle Scholar
Onning, G, Wang, Q, Westrom, BR, Asp, NG & Karlsson, SW (1996) Influence of oat saponins on intestinal permeability in vitro and in vivo in the rat. British Journal of Nutrition 76, 141–151.CrossRefGoogle Scholar
Park, HJ, Kwon, SH, Lee, JH, Lee, KH, Miyamoto, K & Lee, KT (2001) Kalopanaxsaponin A is a basic saponin structure for the anti-tumor activity of hederagenin monodesmosides. Planta Medica 67, 118–121.Google Scholar
Pawar, R, Gopalakrishnan, C & Bhutani, KK (2001) Dammarane triterpene saponin from Bacopa monniera as the superoxide inhibitor in polymorphonuclear cells. Planta Medica 67, 752–754.CrossRefGoogle ScholarPubMed
Petit, PR, Sauvaire, Y, Ponsin, G, Manteghetti, M, Fave, A & Ribes, G (1993) Effects of a fenugreek seed extract on feeding behaviour in the rat: metabolic endocrine correlates. Pharmacology Biochemistry and Behaviour 45, 369–374.Google Scholar
Petit, PR, Sauvaire, YD, Hillairebuys, DM, Leconte, OM, Baissac, YG, Ponsin, GR & Ribes, GR (1995) Steroid saponins from fenugreek seeds – extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol. Steroids 60, 674–680.CrossRefGoogle ScholarPubMed
Pillion, DJ, Amsden, JA, Kensil, CR & Recchia, J (1996) Structure-Function relationship among Quillaja saponins serving as excipients for nasal and ocular delivery of insulin. Journal of Pharmaceutical Sciences 85, 518–524.Google Scholar
Plock, A, Sokolowska-Kohler, W & Presber, W (2001) Application of flow cytometry and microscopical methods to characterize the effect of herbal drugs on Leishmania spp. Experimental Parasitology 97, 141–153.CrossRefGoogle ScholarPubMed
Plohmann, B, Bader, G, Hiller, K & Franz, G (1997) Immunomodulatory and antitumoral effects of triterpenoid saponins. Pharmazie 52, 953–957.Google Scholar
Podolak, I, Elas, M & Cieszka, K (1998) In vitro antifungal and cytotoxic activity of triterpene saponosides and quinoid pigments from Lysimachia vulgaris L. Phytotherapy Research 12, S70–S73.3.0.CO;2-9>CrossRefGoogle Scholar
Potter, SM, Jimenez-Flores, R, Pollack, J, Lone, TA & Berber-Jimenez, MD (1993) Protein saponin interaction and its influence on blood lipids. Journal of Agricultural and Food Chemistry 41, 1287–1291.CrossRefGoogle Scholar
Price, KR, Morgan, C, Gee, JM, Wortley, G, Johnson, IT & Fenwick, GR (1994) Structure activity relationships for saponins and gut permeability, In Proceedings of the International Euro Food TOX IV Conference `Bioactive substances in food of plant origin', 1994, vol. 2, pp. 382–385. Olsztyn, Poland: Polish Academy of Sciences, Centre for Agrotechnology and Veterinary SciencesGoogle Scholar
Punnonen, R & Lukola, A (1980) Oestrogen-like effect of ginseng. British Medical Journal 281, 1110.CrossRefGoogle ScholarPubMed
Quin, G-W & Xu, R-S (1998) Recent advances in bioactive natural products from Chinese medicinal plants. Medical Research Reviews 18, 375–382.3.0.CO;2-8>CrossRefGoogle Scholar
Rao, AV & Sung, M-K (1995) Saponins as anticarcinogens. Journal of Nutrition 125, 717S–724S.Google ScholarPubMed
Recchia, J, Lurantos, MHA, Amsden, JA, Storey, J & Kensil, CR (1995) A semisynthetic Quillaja saponin as a drug delivery agent for aminoglycosaide antibiotics. Pharmaceutical Research 12, 1917–1923.Google Scholar
Rhiouani, H, Settaf, A, Lyoussi, B, Cherrah, Y & Lacaille-Dubois, A (1999) Effects of saponins from Herniaria glabra on blood pressure and renal function in spontaneously hypertensive rats. Therapie 54, 735–739.Google Scholar
Riguera, R (1997) Isolating bioactive compounds from marine organisms. Journal of Marine Biotechnology 5, 187–193.Google Scholar
Ronnberg, B, Fekadu, M, Behboudi, S, Kenne, L & Morein, B (1997) Effects of carbohydrate modification of Quillaja saponaria Molina QH-B fraction on adjuvant activity, cholesterol-binding capacity and toxicity. Vaccine 15, 1820–1826.Google Scholar
Roy, PK & Munshi, JD (1989) Effect of saponin extracts on oxygen uptake and haematology of an air-breathing climbing perch, Anabas testudineus (Bloch). Journal of Freshwater Biology 1, 167–172.Google Scholar
Roy, PK, Munshi, JD & Dutta, HM (1990) Effect of saponin extracts on morpho-history and respiratory physiology of an air breathing fish, Heteropneustes fossilis (Bloch). Journal of Freshwater Biology 2, 135–145.Google Scholar
Rudakewich, M, Ba, F & Benishin, CG (2001) Neurotrophic and neuroprotective actions of Ginsenosides Rb(1) and Rg(1). Planta Medica 67, 533–537.Google Scholar
Santos, WR, Bernardo, RR, Pecanha, LMT, Palatnic, M, Parente, JP & de Sousa, CB (1997) Haemolytic activities of plant saponins and adjuvants. Effect of Periandra mediterranea saponin on the humoral response to the FML antigen of Leishmania donovani. Vaccine 15, 1024–1029.CrossRefGoogle Scholar
Sasaki, S, Sumino, K, Hamajima, K, Fukushima, J, Ishii, N, Kawamoto, S, Mohri, H, Kensil, CR & Okuda, K (1998) Induction of systemic and mucosal immune responses to human immunodeficiency virus type 1 by a DNA vaccine formulated with QS-21 saponin adjuvant via intramuscular and intranasal routes. Journal of Virology 72, 4931–4939.CrossRefGoogle ScholarPubMed
Schopke, T (2000) Non-NMR methods for structure elucidation of saponins. In Saponins in Food, Feedstuffs and Medicinal Plants, Annual Proceedings of the Phytochemical Society, pp. 106–113 [Oleszek, W and Marston, A, editors]. Oxford and London: Clarendon Press.Google Scholar
Seeman, P (1974) Ultrastructure of membrane lesions in immune lysis, osmotic lysis and drug-induced lysis. Federation Proceedings 33, 2116–2124.Google Scholar
Seeman, P, Cheng, D & Iles, GH (1973) Structure of membrane holes in osmotic and saponin hemolysis. Journal of Cell Biology 56, 519–527.Google Scholar
Segal, R, Shatkovsky, P & Milo-Goldzweig, I (1974) On the mechanism of saponin hemolysis -1. Hydrolysis of the glycosidic bond. Biochemical Pharmacology 23, 973–981.Google Scholar
Sen, S, Makkar, HPS & Becker, K (1998) Alfalfa saponins and their implication in animal nutrition. Journal of Agricultural and Food Chemistry 46, 131–140.CrossRefGoogle ScholarPubMed
Shim, I, Javaid, JI & Kim, SE (2000) Effect of ginseng total saponin on extracellular dopamine release elicited by local infusion of nicotine into the striatum of freely moving rats. Planta Medica 66, 705–708.Google Scholar
Shimoyamada, M, Ikedo, S, Ootsubo, R & Watanabe, K (1998) Effects of soybean saponins on chymotryptic hydrolyses of soybean proteins. Journal of Agricultural and Food Chemistry 46, 4793–4797.Google Scholar
Shimoyamada, M, Suzuki, M, Sonta, H, Maruyama, M & Okubo, K (1990) Antifungal activity of saponin fraction obtained from Asparagus Officinalis L. and its active principle. Agricultural and Biological Chemistry 54, 2553–2557.Google Scholar
Sim, JS, Kitts, WD & Bragg, DB (1984) Effect of dietary saponin on egg cholesterol level and laying hen performance. Canadian Journal of Animal Science 64, 977–984.CrossRefGoogle Scholar
Sindambiwe, JB, Calomme, M, Geerts, S, Pieters, L, Vlietinck, AJ & Vanden Berghe, DA (1998) Evaluation of biological activities of triterpenoid saponins from Maesa lanceolata. Journal of Natural Products 61, 585–590.CrossRefGoogle ScholarPubMed
Sjolander, A, Cox, JC & Barr, IG (1998) ISCOMs: an adjuvant with multiple functions (Review). Journal of Leukocyte Biology 64, 713–723.Google Scholar
So, HS, Yoon, HS, Kwoon, YS, Sung, JH, Lee, TG, Park, EN, Cho, HS, Lee, BM, Cho, JM & Ryu, WS (1997) Effect of a novel saponin adjuvant derived from Quillaja saponaria on immune response to recombinant hepatitis B surface antigen. Molecular Biology of Cells 7, 176–186.Google ScholarPubMed
Southon, S, Johnson, IT, Gee, JM & Price, KR (1988) The effect of Gypsophylla saponins in the diet on mineral status and plasma cholesterol concentration in the rat. British Journal of Nutrition 59, 49–55.Google Scholar
Staprans, I, Rapp, JH, Pan, XM, Kim, KY & Feingold, KR (1994) Oxidized lipids in the diet are a source of oxidized lipid in chylomicrons of human serum. Arteriosclerosis and Thrombosis 14, 1900–1905.Google Scholar
Stark, A & Madar, Z (1993) The effect of an ethanol extract derived from fenugreek (Trigonella foenum-graecum) on bile acid absorption and cholesterol level in rats. British Journal of Nutrition 69, 277–287.Google Scholar
Steurer, S, Wurglics, M, Likussar, W, Burmistrov, K, Michelitsch, A & Schubert-Zsilavecz, M (1999) Lack of correlation between surface and interfacial activities of saponins and their haemolytic properties. Pharmazie 54, 766–767.Google Scholar
Stolzenberg, SJ & Parkhurst, RM (1976) Blastocidal and contraceptive actions by an extract and compounds from endod (Phytolacca dodecandra). Contraception 14, 39–51.CrossRefGoogle ScholarPubMed
Story, JA, LePage, SL & Petro, MS (1984) Interactions of alfalfa plant and sprout saponins with cholesterol in vitro and in cholesterol-fed rats. American Journal of Clinical Nutrition 39, 917–929.Google Scholar
Sugano, M, Goto, S, Yamada, Y, Yoshido, K, Hashimoto, Y, Matsuo, T & Kimoto, M (1990) Cholesterol lowering activity of various undigested fractions of soybean protein in rats. Journal of Nutrition 120, 977–985.Google Scholar
Takechi, M, Matsunami, S, Nishizawa, J, Uno, C & Tanaka, Y (1999) Haemolytic and antifungal activities of saponins or anti-ATPase and antiviral activities of cardiac glycosides. Planta Medica 65, 585–586.Google Scholar
Takechi, M & Tanaka, Y (1995a) Structure-activity relationship of synthetic methyl urosylate glycosides. Phytochemistry 34, 675.Google Scholar
Takechi, M & Tanaka, Y (1995b) Haemolytic and time course differences between steroid and triterpenoid saponins. Planta Medica 61, 76–77.Google Scholar
Tamura, K, Honda, H, Mimaki, Y, Sashida, Y & Kogo, H (1997) Inhibitory effects of a new steroidal saponin, OSW-1, on ovarian function in rats. British Journal of Pharmacology 121, 1796–1802.Google Scholar
Teferedegne, B (2000) New perspectives on the use of tropical plants to improve ruminant nutrition. Proceedings of the Nutrition Society 59, 209–214.CrossRefGoogle ScholarPubMed
Terapunduwat, S & Tasaki, I (1986) Effect of dietary saponin on the performance and plasma cholesterol level of chicks and the alleviation of saponin toxicity by cholesterol supplementation. Japanese Journal of Zootechnical Science 57, 524–533.Google Scholar
Tewary, PV, Chaturvedi, C & Pandey, VB (1973) Antifertility activity of Costus speciosus Sm. Indian Journal of Pharmacology 35, 114–115.Google Scholar
Traore, F, Faure, R, Ollivier, E, Gasquet, M, Azas, N, Debrauwer, L, Keita, A, Timon-David, P & Balansard, G (2000) Structure and antiprotozoal activity of triterpenoid saponins from Glinus oppositifolius. Planta Medica 66, 368–371.CrossRefGoogle ScholarPubMed
Ursini, F, Zamburlini, A, Cazzolato, G, Maiorino, M, Bon, GB & Sevanian, A (1998) Postprandial plasma lipid hydroperoxides: a possible link between diet and atherosclerosis. Free Radical Biology and Medicine 25, 250–252.Google Scholar
Wakabayashi, C, Hasegawa, H, Murata, J & Saiki, I (1997) In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncology Research 9, 411–417.Google ScholarPubMed
Wakabayashi, C, Murakami, K, Hasegawa, H, Murata, J & Saiki, I (1998) An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells. Biochemical and Biophysical Research Communications 246, 725–730.CrossRefGoogle ScholarPubMed
Wallace, RJ, Arthaud, L & Newbold, CJ (1994) Influence of Yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms. Applied Environmental Microbiology 60, 1762–1767.CrossRefGoogle ScholarPubMed
Wang, Y, McAllister, TA, Yanke, LJ & Cheeke, PR (2000a) Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes. Journal of Applied Microbiology 88, 887–896.Google Scholar
Wang, YX, McAllister, TA, Yanke, LJ, Xu, ZJ, Cheeke, PR & Cheng, KJ (2000 b) In vitro effects of steroidal saponins from Yucca schidigera extract on rumen microbial protein synthesis and ruminal fermentation. Journal of the Science of Food and Agriculture 80, 2114–2122.Google Scholar
Woldemichael, GM & Wink, M (2001) Identification and biological activities of triterpenoid saponins from Chenopodium quinoa. Journal of Agricultural and Food Chemistry 49, 2327–2332.CrossRefGoogle ScholarPubMed
Wolff, SP & Nourooz-Zadeh, J (1996) Hypothesis: UK consumption of dietary lipid hydroperoxides – a possible contributory factor to atherosclerosis. Atherosclerosis 119, 261–263.Google Scholar
World Health Organization (1996) The World Health Report. Geneva: WHO.Google Scholar
Wu, Z, Sadik, M & Sleiman, FT (1994) Influence of Yucca extract on ruminal metabolism in cows. Journal of Animal Science 72, 1038–1042.CrossRefGoogle ScholarPubMed
Yamasaki, Y, Ito, K, Enomoto, Y & Sutko, JL (1987) Alterations by saponins of passive calcium permeability and sodium-calcium exchange activity of canine cardiac sarcolemnal vesicles. Biochimica et Biophysica Acta 897, 481–487.Google Scholar
Yoshikawa, M, Murakami, T, Kishi, A, Kageura, T & Matsuda, H (2001) Medicinal flowers. III. Marigold (1): hypoglycaemic, gastric emptying inhibitory, and gastroprotective principles and new oleanane-type triterpene oligoglycosides, calendasaponins A, B, C, and D, from Egyptian Calendula officinalis. Chemical and Pharmaceutical Bulletin 49, 863–870.Google Scholar
Yoshiki, Y, Kudou, S & Okubo, K (1998) Relationship between chemical structures and biological activities of triterpenoid saponins from soybean (Review). Bioscience Biotechnology and Biochemistry 62, 2291–2299.CrossRefGoogle Scholar
Yoshiki, Y & Okubo, K (1995) Active oxygen scavenging activity of DDMP (2,3-dihydro-2, 5-dihydroxy-6-methyl-4H-pyran-4-one) saponin in soybean seed. Bioscience Biotechnology and Biochemistry 59, 1556–1557.Google Scholar
Yoshikoshi, M, Kahara, T, Yoshiki, Y, Ito, M, Furukawa, Y, Okubo, K & Amarowicz, R (1995) Metabolism and nonabsorption of soybean hypocotyl saponins in the rat model. Acta Alimentaria 24, 355–364.Google Scholar
Yui, S, Ubukata, K, Hodono, K, Kitahara, M, Mimaki, Y, Kuroda, M, Sashida, Y & Yamazaki, M (2001) Macrophage-oriented cytotoxic activity of novel triterpene saponins extracted from roots of Securidaca inappendiculata. International Immunopharmacology 1, 1989–2000.Google Scholar
Zhang, YW, Dou, DQ, Zhang, L, Chen, YJ & Yao, XS (2001) Effects of Ginsenosides from Panax ginseng on cell-to-cell communication function mediated by gap junctions. Planta Medica 67, 417–422.Google Scholar
Zhao, RZ & McDaniel, WF (1998) Ginseng improves strategic learning by normal and brain-damaged rats. NeuroReport 9, 1619–1624.Google Scholar
Zilversmit, DB (1979) Atherogenesis: a postprandial phenomenon. Circulation 60, 473–485.Google Scholar