Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-03T05:31:56.117Z Has data issue: false hasContentIssue false

Availability of lysine in vegetable protein concentrates as determined by the slope-ratio assay with growing pigs and rats and by chemical techniques

Published online by Cambridge University Press:  09 March 2007

E. S. Batterham
Affiliation:
NSW Department of Agriculture, Agricultural Research Centre, Wollongbar, New South Wales 2480, Australia
R. D. Murison
Affiliation:
NSW Department of Agriculture, Agricultural Research Centre, Wollongbar, New South Wales 2480, Australia
R. F. Lowe
Affiliation:
NSW Department of Agriculture, Agricultural Research Centre, Wollongbar, New South Wales 2480, Australia
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The availability of lysine in seven vegetable protein concentrates was determined using the slope-ratio assay with growing pigs and rats and with two chemical techniques.

2. With pigs, estimates of availability were lower using carcass compared to live-weight values, but there was no consistent effect of including food intake in the criterion of response. Availability estimates, using food conversion efficiency on a carcass basis were (proportion of total) linseed meal − 0·23, lupin-seed meal 0.74, rapeseed meal no. 1 0.97, no. 2 0.77, sunflower meal no. 1 0.59, no. 2 0.66, no. 3 0.54.

3. Availability estimates for lysine with rats were also lower using carcass compared to live-weight values. Estimates based on food conversion efficiency were higher than those based on weight gain. Availability estimates in lupin-seed meal and sunflower meals were in general agreement with the pig estimates, whilst that with linseed meal was considerably higher (0·82). Assays were not conducted with rapessed meal due to problems of food rejection with this meal.

4. The differences in available lysine were not detected by the chemical Silcock available-lysine test (Roach et al. 1967) nor by the direct fluoro-dinitrobenzene procedure (Carpenter, 1960).

Type
Papers on General Nutrition
Copyright
Copyright © The Nutrition Society 1981

References

REFERENCES

Batterham, E. S., Murison, R. D. & Lewis, C. E. (1978). Bri. J. Nutr. 40, 23.CrossRefGoogle Scholar
Batterham, E. S., Murison, R. D. & Lewis, C. E. (1979). Br. J. Nutr. 41, 383.CrossRefGoogle Scholar
Batterham, E. S. & O'Neill, G. H. (1978). Br. J. Nutr. 39, 265.CrossRefGoogle Scholar
Carpenter, K. J. (1960). Biochem. J. 77, 604.CrossRefGoogle Scholar
Finney, D. J. (1964). Statistical Method in Biological Assay, 2nd ed. London: Griffin.Google Scholar
Lewis, D. & Cole, D. J. A. (1976). Proc. Nutr. Soc. 35, 87.CrossRefGoogle Scholar
Liener, I. E. (1969). Toxic Constituents of Plant Foodstuffs. New York: Academic Press.Google Scholar
National Research Council (1972). Nutrient Requirements of Laboratory Animals, 2nd ed. Washington DC: National Academy of Sciences.Google Scholar
Roach, A. G., Sanderson, P. & Williams, D. R. (1967). J. Sci. Fd Agric. 18, 274.CrossRefGoogle Scholar
Simmons, N. O. (1963). Feed Milling and Associated Subjects, 2nd ed. London: Leonard Hill (Books) Ltd.Google Scholar
Taverner, M. R. & Rayner, C. J. (1975). Aust. J. exp. Agric. Anim. Husb. 15, 626.CrossRefGoogle Scholar