Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-25T03:15:26.419Z Has data issue: false hasContentIssue false
  • English
  • Français

The assessment of zinc status of an animal from the uptake of 65Zn by the cells of whole blood in vitro

Published online by Cambridge University Press:  24 July 2007

J. K. Chesters  and
Marie Will
J. K. Chesters
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Marie Will
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. 65Zn uptake by blood cells in vitro has been compared with plasma Zn concentration and plasma alkaline phosphatase (EC 3.1.3.1) activity as indicators of an animal's Zn status.

2. Dietary Zn deficiency, low food intake, reduced dietary protein content and endotoxin administration all reduced plasma Zn concentration in the rat. In each case there was a parallel reduction in plasma alkaline phosphatase activity and an increase in 65Zn uptake in vitro by cells of whole blood.

3. A similar relationship between the three measurements existed in sheep with lowered plasma Zn concentrations whether these were caused by dietary deficiency or by post-surgical stress.

4. 65Zn uptake by cells of whole blood did not differentiate dietary Zn deficiency from the other factors which reduce plasma Zn under ‘field’ conditions.

5. 65Zn uptake by the cells in whole blood in vitro was three to five times less rapid in blood of ruminant origin than in that from non-ruminants. This difference related to the erythrocytes rather than to the leukocytes or the plasma.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 39 , Issue 2 , March 1978 , pp. 297 - 306
Copyright
Copyright © The Nutrition Society 1978

References

REFERENCES

Allcroft, W. M. & Folley, S. J. (1941). Biochem. J. 35, 254.CrossRefGoogle Scholar
Berry, R. K., Bell, M. C. & Wright, P. L. (1966). J. Nutr. 88, 284.CrossRefGoogle Scholar
Chesters, J. K. & Will, M. (1973). Br. J. Nutr. 30, 555.CrossRefGoogle Scholar
Hambidge, K. M. & Droegemueller, W. (1974). Obstet. Gynec. N. Y. 44, 666.Google Scholar
Healy, P. J. & McInnes, P. (1975). Res. vet. Sci. 18, 157.CrossRefGoogle Scholar
Hove, E., Elvehjem, C. A. & Hart, E. B. (1940). J. biol. Chem. 136, 425.CrossRefGoogle Scholar
Lassiter, J. W. & Morton, J. D. (1968). J. Anim. Sci. 27, 776.CrossRefGoogle Scholar
Lindeman, R. D., Bottomley, R. G., Cornelison, R. L., & Jacobs, L. A. (1972). J. Lab. clin. Med. 79, 452.Google Scholar
Loveless, B. W. & Heaton, F. W. (1976). Br. J. Nutr. 36, 487.CrossRefGoogle Scholar
Miller, E. R., Luecke, R. W., Ullrey, D. E., Baltzer, B. V., Bradley, B. L. & Hoeffer, J. A. (1968). J. Nutr. 95, 278.CrossRefGoogle Scholar
Miller, W. J., Pitts, W. J., Clifton, C. M. & Morton, I. D. (1969). J. Dairy Sci. 48, 1329.CrossRefGoogle Scholar
Mills, C. F. (1973). In Therapeutic Aspects of Nutrition, p. 14 [Jonxis, J. H. P., Visser, H. K. A. and Troelsta, J. A., editors]. Leiden: Stenfert Kroese.CrossRefGoogle Scholar
Mills, C. F., Quarterman, J., Chesters, J. K., Williams, R. B. & Dalgarno, A. C. (1969). Am. J. clin. Nutr. 22, 1240.CrossRefGoogle Scholar
Nelson, G. J. (1967). Biochim. biophys. Acta 144, 221.CrossRefGoogle Scholar
Pekarek, R. S. & Beisel, W. R. (1969). Appl. Microbiol. 18, 482.CrossRefGoogle Scholar
Pekarek, R. S., Burghen, G. A., Bartelloni, P. J., Calia, F. M., Bostian, K. A. & Beisel, W. R. (1970). J. Lab. clin. Med. 76, 293.Google Scholar
Sasser, L. B., Bell, M. C. & Jarboe, G. E. (1975). J. Anim. Sci. 41, 1679.CrossRefGoogle Scholar
Sigma Chemical Co. (1974). Sigma Technical Bulletin no. 104. St Louis, Missouri: Sigma Chemical Co.Google Scholar
Tupper, R., Watts, R. W. E. & Wormall, A. (1952). Biochem. J. 50, 429.CrossRefGoogle Scholar
Van Campen, D. & House, W. A. (1974). J. Nutr. 104, 84.CrossRefGoogle Scholar
Vikbladh, I. (1951). Scand. J. clin. Lab. Invest. 3, Suppl. 2.Google Scholar
Williams, R. B. & Mills, C. F. (1970). Br. J. Nutr. 24, 989.Google Scholar
Zwaal, R. F. A., Roelofsen, B. & Calley, C. M. (1973). Biochim. biophys. Acta 300, 159.CrossRefGoogle Scholar