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Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep

Published online by Cambridge University Press:  09 March 2007

T. H. Terrill ,
G. C. Waghorn ,
D. J. Woolley ,
W. C. Mcnabb  and
T. N. Barry
T. H. Terrill
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
G. C. Waghorn
Affiliation:
AgResearch Grasslands, Private Bag 11008, Palmerston North, New Zealand
D. J. Woolley
Affiliation:
Department of Plant Science, Massey University, Palmerston North, New Zealand
W. C. Mcnabb
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
T. N. Barry
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
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Abstract

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Three experiments were conducted to determine the fate of condensed tannins (CT) during digestion in sheep. CT were measured as extractable, protein-bound and fibre-bound fractions using the butanol-HCI procedure. In Expt 1, purified CT were added to digesta from different parts of the digestive tract obtained from a pasture-fed sheep. Recoveries of CT after 0 and 4 h of anaerobic incubation at 39° averaged: rumen 78.9 and 57.5 %; abomasum 50.9 and 49.0 %; duodenum 64.4 and 46.0 % and ileum 43.4 and 38.8%. In Expt 2, [14C]CT was given per abomasum over a 6.5 h period at 15 min intervals to a sheep previously fed on Lotus pedunculatus (which contains CT). The sheep was killed at the end of the period and 92.4% of the label was recovered. Virtually all of the label was in the digesta, and none was detected in the blood, so that the CT-carbon appeared not to be absorbed from the small intestine. In Expt 3, rumen, abomasal and ileal digesta and faeces samples from sheep fed on Lotus pedunculatus were analysed for CT and CT flow along the digestive tract calculated from reference to indigestible markers. Values were low in all digesta samples, indicating disappearance of CT across the rumen and small intestine, and CT recovery in faeces was only about 15% of intake. However, the 14C results from Expt 2 suggested that little if any CT-carbon was absorbed and the low recoveries in Expt 1 are considered to be a consequence of either conformational changes to the CT molecule such that it is no longer detectable by colorimetric methods, an inability of the analytical method to release bound CT for the butanol–HCI assay, or interference from other digesta constituents. It is concluded that the butanol–HCI method of CT analysis is appropriate for quantifying CT in herbages but not in digesta or faeces, and that a substantial part of CT released during protein digestion in the small intestine may not be detectable by normal CT analytical methods.

Keywords

[144C]Condensed tanninDigesta tanninsTannin digestion
Type
Assay and digestion of labelled tannis
Information
British Journal of Nutrition , Volume 72 , Issue 3 , September 1994 , pp. 467 - 477
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Barry, T. N. (1989). Condensed tannins; their role in ruminant protein and carbohydrate digestion and possible effects upon the rumen ecosystem. In The Role of Protozoa and Fungi in Ruminant Digestion, pp. 153–169 [Nolan, J. V., Leng, R. A. and Demeyer, D. I., editors]. Armidale, Australia: Penambal Books.Google Scholar
Bate-Smith, E. C. (1973). Tannins of herbaceous leguminosae. Phytochemistry 12, 1809–1812.CrossRefGoogle Scholar
Broadhurst, R. B. & Jones, W. T. (1978). Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture 29, 788–794.CrossRefGoogle Scholar
Foo, L. Y., Jones, W. T., Porter, L. J. & Williams, V. M. (1982). Proanthocyanidin polymers of fodder legumes. Phytochemistry 21, 933–935.Google Scholar
Hinks, N. T., Mills, S. C. & Setchell, B. P. (1966). A simple method for the determination of specific activity of carbon dioxide in blood. Analytical Biochemistry 17, 551–553.CrossRefGoogle ScholarPubMed
Jones, W. T., Broadhurst, R. B. & Lyttleton, J. W. (1976). The condensed tannins of pasture legume species. Phytochemistryl 15, 1407–1409.CrossRefGoogle Scholar
Jones, W. T. & Mangan, J. L. (1977). Complexes of the condensed tannins of the sainfoin (Onobrychis viciifolia Scop.) with Fraction 1 leaf protein and with submaxillary muco-protein, and their reversal by polyethylene glycol and pH. Journal of the Science of Food and Agriculture 28, 126–136.CrossRefGoogle Scholar
Lai, R., Woolley, D. J. & Lawes, G. S. (1988). Patterns of assimilate transport from leaves to fruit within a kiwifruit (Acfinidia deliciosa) lateral. Journal of Horticultural Science 63, 725–730.CrossRefGoogle Scholar
McNabb, W. C., Waghorn, G. C., Barry, T. N. & Shelton, I. D. (1993). The effect of condense tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. British Journal of Nutrition 70, 647–661.CrossRefGoogle ScholarPubMed
Mangan, J. L. (1988). Nutritional effects of tannins in animal feeds. Nutrition Research Reviews 1, 209–231.CrossRefGoogle ScholarPubMed
Porter, L. J., Hrstich, L. N. & Chan, B. C. (1986). The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 25, 223–230.CrossRefGoogle Scholar
Sarkar, S. K., Howarth, R. E. & Golpen, B. P. (1976). Condensed tannins in herbaceous legumes. Crop Science 16, 543–546.CrossRefGoogle Scholar
Terrill, T. H., Douglas, G. B., Foote, A. G., Purchas, R. W., Wilson, G. F. & Barry, T. N. (1992 a). Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal of Agricultural Science, Cambridge 119, 265–273.CrossRefGoogle Scholar
Terrill, T. H., Rowan, A. M., Douglas, G. B. & Barry, T. N. (1992 b). Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture 58, 321–329.CrossRefGoogle Scholar
Terrill, T. H., Windham, W. R., Evans, J. J. & Hoveland, C. S. (1990). Condensed tannin concentrations in Sericea lespedeza, as influenced by preservation method. Crop Science 30, 219–224.CrossRefGoogle Scholar
Terrill, T. H., Windham, W. R., Hoveland, C. S. & Amos, H. E. (1989). Forage preservation method influences on tannin concentration, intake and digestibility of Sericea Iespedeza by sheep. Agronomy Journal 81, 435–439.CrossRefGoogle Scholar
Waghorn, G.C., Shelton, I. D. & McNabb, W. C. (1994 a). Effects of condensed tannins in Lotuspedunculctus on its nutritive value for sheep. 1. Non-nitrogenous aspects. Journal of Agricultural Science, Cambridge 123, 99–107.CrossRefGoogle Scholar
Waghorn, G.C., Shelton, I. D., McNabb, W. C. & McCutcheon, S. N. (1994 b). Effects of condensed tannin in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. Journal of Agricultural Science, Cambridge 123, 109–119.CrossRefGoogle Scholar
Yn, F., Barry, T. N., Moughan, P. J. & Wilson, G.F. (1993). The concentration of condensed tannin and gossypol in cottonseed and in processed cottonseed meal. Journal of the Science of Food and Agriculture 63, 7–15.Google Scholar