Hostname: page-component-745bb68f8f-v2bm5 Total loading time: 0 Render date: 2025-01-24T11:11:54.192Z Has data issue: false hasContentIssue false
  • English
  • Français

Application of selective extraction to the study of iron species present in diet and rat gastrointestinal tract contents

Published online by Cambridge University Press:  09 March 2007

Robert J. Simpson ,
Sanjiv Sidhar  and
Timothy J. Peters
Robert J. Simpson
Affiliation:
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, Bessemer Road, London SE5 9PJ
Sanjiv Sidhar
Affiliation:
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, Bessemer Road, London SE5 9PJ
Timothy J. Peters
Affiliation:
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, Bessemer Road, London SE5 9PJ
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Iron speciation in rodent diet and rat gastrointestinal tract lumen during dietary digestion and absorption was investigated with a novel selective extraction technique. Five Fe fractions were identified, namely exchangeable (soluble in 1 M-magnesium chloride), carbonate-bound (soluble in mild acid), oxide-bound (soluble in hydroxylamine–acetic acid), organic-bound (soluble after treatment with peroxide in nitric acid) and residual. Fe from the pelleted diet was mobilized by rat stomach to the exchangeable fraction, then redistributed to the carbonate- and oxide-bound fractions on passage through the proximal small intestine. In vitro incubation of diet with hydrochloric acid failed to mimic the in vivo effect of the stomach. In vitro neutralization of stomach contents with bicarbonate was found to produce a similar effect on Fe speciation to that seen when diet passed the proximal small intestine in vivo. Comparison of 59Fe speciation in extrinsically labelled diet with endogenous Fe speciation showed that extrinsic labelling does not uniformly label all endogenous species. The experiments suggest that selective extraction may provide a useful approach to the study of Fe species present in diets, in vitro digestions and gastrointestinal contents.

Keywords

Iron speciationSelective extraction techniqueRodent diet
Type
Absorption of Inorganic Nutrients
Information
British Journal of Nutrition , Volume 67 , Issue 3 , May 1992 , pp. 437 - 444
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

Becker, G., Korpilla-Schafer, S., Osterloh, K. & Forth, W. (1979) Capacity of the mucosal transfer system and absorption of iron after oral administration in rats. Blut 38, 127–134.CrossRefGoogle ScholarPubMed
Brown, E. B. & Justus, B. W. (1958) In vitro absorption of radioiron by everted pouches of rat intestine. American Journal of Physiology 194, 319–326.CrossRefGoogle ScholarPubMed
Cook, J. D., Layrisse, M., Martinez-Torres, C., Walker, R., Monsen, E. & Finch, C. A. (1972) Food iron absorption measured by an extrinsic tag. Journal of Clinical Investigation 51, 805–815.CrossRefGoogle ScholarPubMed
Derman, D. P., Bothwell, T. H., Torrance, J. D., Macphail, A. P., Bezwoda, W. R., Charlton, R. W. & Mayet, F. G. H. (1982) Iron absorption from ferritin and ferric hydroxide. Scandinavian Journal of Haematology 29, 18–24.CrossRefGoogle ScholarPubMed
Fairweather-Tait, S. J. (1987) The concept of bioavailability as it relates to iron metabolism. Nutrition Research 7, 319–325.CrossRefGoogle Scholar
Glover, J. & Jacobs, A. (1971) Observations on iron in the jejunal lumen after a standard meal. Gut 12, 369–371.CrossRefGoogle ScholarPubMed
Hodson, A. Z. (1970) Conversion of ferric to ferrous iron in weight control dietaries. Journal of Agricultural and Food Chemistry 18, 946–947.CrossRefGoogle ScholarPubMed
Jacobs, A. & Greenman, D. A. (1969) Availability of food iron. British Medical Journal 1, 673–676.CrossRefGoogle ScholarPubMed
Johnson, G., Jacobs, P. & Purves, L. R. (1983) Iron binding proteins of iron absorbing rat intestinal mucosa. Journal of Clinical Investigation 71, 1467–1476.CrossRefGoogle ScholarPubMed
Layrisse, M., Martinez-Torrez, C., Renzy, M. & Leets, I. (1975) Ferritin iron absorption in man. Blood 45, 689–698.CrossRefGoogle ScholarPubMed
Lee, K. & Clydesdale, F. M. (1979) Quantitative determination of elemental, ferrous, ferric, soluble and complexed iron in foods. Journal of Food Science 44, 549–554.CrossRefGoogle Scholar
Lock, S. & Bender, A. E. (1980) Measurement of chemically-available iron in foods by incubation with human gastric juice in vitro. British Journal of Nutrition 43, 413–420.CrossRefGoogle ScholarPubMed
Lynch, S. R. (1984). Iron. In Absorption and Malabsorption of Mineral Nutrients, pp. 89–124. New York: Alan R. Liss.Google Scholar
Miller, D. D., Schricker, B. R., Rasmussen, R. R. & Van Campen, D. (1981) An in vitro method for estimation of iron availability from meals. American Journal of Clinical Nutrition 34, 2248–2256.CrossRefGoogle Scholar
Narisingha Rao, B. S. & Prabhavathi, T. (1978) An in vitro method for predicting the bioavailability of iron from foods. American Journal of Clinical Nutrition 31, 169–175.CrossRefGoogle Scholar
Reddy, M. B., Chidambaram, M. V., Fonseca, J. & Bates, G. W. (1986) Potential role of in vitro iron bioavailability studies in combatting iron deficiency: A study of the effects of phosvitin on iron mobilisation from pinto beans. Clinical Physiology and Biochemistry 4, 78–86.Google ScholarPubMed
Selden, C. & Peters, T. J. (1986) Separation and assay of iron proteins in needle biopsy specimens of human liver. Clinica Chimica Acta 98, 47–52.CrossRefGoogle Scholar
Shackleton, L. & McCance, R. A. (1936) The ionisable iron in foods. Biochemical Journal 30, 582–591.CrossRefGoogle ScholarPubMed
Simpson, R. J. & Peters, T. J. (1986). Mouse intestinal uptake kinetics in vivo. The significance of brush-border membrane vesicle transport in the mechanism of mucosal Fe3+ uptake. Biochimica et Biophysica Acta 856, 115–122.CrossRefGoogle Scholar
Simpson, R. J. & Peters, T. J. (1990) Forms of soluble iron in mouse stomach and duodenal lumen: significance for mucosal uptake. British Journal of Nutrition 63, 79–89.CrossRefGoogle ScholarPubMed
Tessier, A., Campbell, P. G. C. & Bisson, M. (1979) Sequential extraction procedure for the speciation of particulate trace metals. Analytical Chemistry 51, 844–851.CrossRefGoogle Scholar
Wheby, M. S. & Crosby, W. H. (1963) The gastrointestinal tract and iron absorption. Blood 22, 416–428.CrossRefGoogle ScholarPubMed