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Anti-atherogenic effect of soya and rice-protein isolate, compared with casein, in apolipoprotein E-deficient mice

Published online by Cambridge University Press:  07 June 2007

Weihua Ni ,
Yasuyuki Tsuda ,
Shinichiro Takashima ,
Hiroyoshi Sato ,
Masao Sato  and
Katsumi Imaizumi
Weihua Ni
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
Yasuyuki Tsuda
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
Shinichiro Takashima
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
Hiroyoshi Sato
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
Masao Sato
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
Katsumi Imaizumi*
Affiliation:
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
*
*Corresponding author: Dr Katsumi Imaizumi, fax +81 92 642 3003, email [email protected]
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Abstract

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Our objective was to determine whether dietary plant proteins such as soya-protein isolate (SPI) and rice-protein isolate (RPI) compared with animal proteins, such as casein, could afford beneficial effects on atherosclerosis development in apolipoprotein E-deficient mice. In experiment 1, male and female mice were fed on a purified diet containing either casein, SPI or RPI for 9 weeks. The en face lesion area in the aorta (P < 0·05) and the lesion size in the aortic root (P < 0·05) in mice fed the casein-based diet were greater than those in the SPI or RPI groups. The plant protein groups had an increased concentration of serum l-arginine (P < 0·05) and NO metabolites (NO2 plus NO3) (P < 0·05) than did the casein group. The inhibitory effect of the plant proteins on the lesion formations was unrelated to gender and total serum cholesterol. In experiment 2, the l-arginine and l-methionine contents were the same in the l-arginine-supplemented casein-based and SPI-based diets, and between the l-methionine-supplemented SPI-based and the casein-based diets. Male mice were fed on the diets for 15 weeks. There were no significant differences in the en face lesion area and the lesion size between the casein group and the l-arginine-supplemented group, although the serum l-arginine (P < 0·05) and NO2 plus NO3 (P < 0·05) concentrations in the supplemented group were higher than those in the casein group. There were no significant effects of l-methionine supplementation on the lesion formations. In experiment 3, male mice were given the casein-based diet or the l-arginine-supplemented casein-based diet together with water or water containing an NO synthesis inhibitor for 9 weeks. When given the casein-based diet, the inhibitor drinking, compared with water drinking, resulted in a reduction of the serum NO2 plus NO3 concentration (P < 0·01) and an increase in the en face lesion area (P < 0·05) and the lesion size (P < 0·01). When given the l-arginine-supplemented diet, the inhibitor drinking, compared with water drinking, resulted in no increase in the lesion area and size. These results demonstrate anti-atherogenic potentials of SPI- as well as RPI-derived proteins, but their l-arginine and l-methionine contents were not sufficient enough to explain the underlying mechanism(s).

Keywords

Apolipoprotein-E-deficient miceL-ArginineAtherosclerosisSoya proteinRice protein
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 1 , July 2003 , pp. 13 - 20
Copyright
Copyright © The Nutrition Society 2003

References

Adams, MR, Golden, DL, Register, TC, et al. (2002) The atheroprotective effect of dietary soy isoflavones in apolipoprotein E-/-mice requires the presence of estrogen receptor-α. Arterioscler Thromb Vasc Biol 22, 1859–1864.CrossRefGoogle ScholarPubMed
Aji, W, Ravalli, S, Szabolcs, M, et al. (1997) L-Arginine prevents xanthoma development and inhibits atherosclerosis in LDL receptor knockout mice. Circulation 95, 430–437.CrossRefGoogle ScholarPubMed
Anderson, JW, Johnstone, BM & Cook-Newwell, ME (1995) Meta-analysis of the effects of soy protein intake on serum lipids. N Engl J Med 333, 276–282.CrossRefGoogle ScholarPubMed
Ando, M, Tomoyori, H & Imaizumi, K (2002) Dietary cholesterol-oxidation products accumulate in serum and liver in apolipoprotein E-deficient mice, but do not accelerate atherosclerosis. Br J Nutr 88, 339–345.CrossRefGoogle Scholar
Beynen, AC (1992) Cholesterolemic effects of dietary soybean protein and casein: mechanism of action. In Dietary Proteins: How They Alleviate Diseases and Promote Better Health. pp 77–83. [Liepa, GU, editor]. Champaign, IL: AOCS Press.CrossRefGoogle Scholar
Bode-Böger, SM, Böger, RH & Creutzig, A (1994) L-arginine infusion decreases peripheral arterial resistance and inhibits platelet aggregation in healthy subjects. Clin Sci 87, 303–310.CrossRefGoogle ScholarPubMed
Böger, RH, Bode-Böger, SM & Frölich, JC (1996) The L-arginine-nitric oxide pathway. Role in atherosclerosis and therapeutic implications. Atherosclerosis 127, 1–11.CrossRefGoogle ScholarPubMed
Böger, RH, Bode-Böger, SM, Mügge, A, et al. (1995) Supplementation of hypercholesterolaemic rabbits with L-arginine reduces the vascular release of superoxide anions and restores NO production. Atherosclerosis 117, 273–284.CrossRefGoogle ScholarPubMed
Böger, RH, Bode-Böger, SM, Phivthong-ngam, L, et al. (1998) Dietary L-arginine and α-tocopherol reduce vascular oxidative stress and preserve endothelial function in hypercholesterolemic rabbits via different mechanisms. Atherosclerosis 141, 31–43.CrossRefGoogle ScholarPubMed
Candipan, RC, Wang, B, Buitrago, R, Tsao, PS & Cooke, JP (1996) Regression or progression: dependency on vascular nitric oxide. Arterioscler Thromb Vasc Biol 16, 44–50.CrossRefGoogle ScholarPubMed
Chang, KC, Lee, CC & Brown, G (1986) Production and nutritional evaluation of high-protein rich flour. J Food Sci 51, 464–467.CrossRefGoogle Scholar
Cooke, JP, Singer, AH, Tsao, PS, Zera, P, Rowan, RA & Billingham, ME (1992) Anti-atherogenic effects of L-arginine in the hypercholesterolemic rabbit. J Clin Invest 90, 1168–1172.CrossRefGoogle Scholar
Cooke, JP & Tsao, PS (1997) Arginine: a new therapy for atherosclerosis?. Circulation 95, 311–312.CrossRefGoogle ScholarPubMed
Hofmann, MA, Lalla, E, Lu, Y, et al. (2001) Hyperhomocysteinemia enhances vascular inflammation and accelerates atherosclerosis in a murine model. J Clin Invest 107, 675–683.CrossRefGoogle ScholarPubMed
Ikeda, A, Wakamatsu, K, Umeda, T, et al. (1994) Effects of dietary protein and fat on linoleic and α-linolenic acid metabolism and prostacyclin production in stroke-prone spontaneous hypertensive rats. J Nutr Biochem 5, 248–255.CrossRefGoogle Scholar
Kirk, EA, Sutherland, PS, Wang, SA, Chait, A & LeBoeuf, RC (1998) Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr 128, 954–959.CrossRefGoogle Scholar
Kuhlencordt, PJ, Chen, J, Han, F, Astern, J & Huang, PL (2001) Genetic deficiency of inducible nitric oxide synthase reduces atherosclerosis and lowers plasma lipid peroxides in apolipoprotein E-knockout mice. Circulation 103, 3099–3104.CrossRefGoogle ScholarPubMed
Miller, L & Houghton, JA (1945) The micro-Kjeldahl determination of the nitrogen content of amino acids and proteins. J Biol Chem 159, 373–380.CrossRefGoogle Scholar
Minor, RLJ, Myers, PR, Guerra, RJ, Bates, JN & Harrison, DG (1990) Diet-induced atherosclerosis increases the release of nitrogen oxides from rabbit aorta. J Clin Invest 86, 2109–2116.CrossRefGoogle ScholarPubMed
Morita, T & Kiriyama, S (1993) Mass production method for rice protein isolate and nutritional evaluation. J Food Sci 58, 1393–1396.CrossRefGoogle Scholar
Morita, T, Oh-hashi, A, Takei, K, Ikai, M, Kasaoka, S & Kiriyama, S (1997) Cholesterol-lowering effects of soybean, potato and rice proteins depend on their low methionine contents in rats fed a cholesterol-free purified diet. J Nutr 127, 470–477.CrossRefGoogle ScholarPubMed
Nakashima, Y, Plump, AS, Raines, EW, Breslow, JL & Ross, R (1994) Apo E-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arteriosclerosis and Thrombosis 14, 133–140.CrossRefGoogle ScholarPubMed
Ni, W-H, Tsuda, Y, Sakono, M & Imaizumi, K (1998) Dietary soy protein isolate, compared with casein, reduces atherosclerosis in apolipoprotein E-deficient mice. J Nutr 128, 1884–1889.CrossRefGoogle Scholar
Paigen, B, Morrow, A, Holmes, PA, Mitchell, D & Williams, RA (1987) Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis 68, 231–240.CrossRefGoogle ScholarPubMed
Potter, SM (1996) Soy protein and serum lipids. Curr Opin Lipidol 7, 260–264.CrossRefGoogle ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.CrossRefGoogle Scholar
Toborek, M, Kopieczna-Grzebieniak, E, Drózdz, M & Wieczorek, M (1995) Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits. Atherosclerosis 115, 217–224.CrossRefGoogle ScholarPubMed
Tsao, PS, McEvoy, LM, Drexler, H, Butcher, EC & Cooke, JP (1994) Enhanced endothelial adhesiveness in hypercholesterolemia is attenuated by L-arginine. Circulation 89, 2176–2182.CrossRefGoogle ScholarPubMed
Wishnok, JS, Glogowski, JA & Tannenbaum, SR (1996) Quantitation of nitrate, nitrite, and nitrosating agents. Methods Enzymol 268, 130–141.CrossRefGoogle ScholarPubMed
Yamakoshi, J, Piskula, MK, Izumi, T, et al. (2000) Isoflavone aglycone-rich extract without soy protein attenuates atherosclerosis development in cholesterol-fed rabbits. J Nutr 130, 1887–1893.CrossRefGoogle ScholarPubMed
Zhang, SH, Reddick, RL, Piedrahita, JA & Maeda, N (1992) Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science 258, 468–471.CrossRefGoogle ScholarPubMed
Zhou, J, Møller, J, Danielsen, CC et al. (2001) Dietary supplementation with methionine and homocysteine promotes early atherosclerosis but not plaque rupture in apo E-deficient mice. Arterioscler Thromb Vasc Biol 21, 1470–1476.CrossRefGoogle ScholarPubMed